Temperature incubations of microbial communities from two different locations in Fram Strait - bulk data
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| Format: | Dataset Open Access |
| Sprache: | en |
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2024
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| _version_ | 1867169477607555072 |
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| author | Ahme, Antonia John, Uwe |
| author_facet | Ahme, Antonia John, Uwe |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | This dataset encompasses data derived from two temperature incubation experiments aboard the RV Polarstern, using a unicellular microbial community collected from different stations in the Fram Strait during the PS126 campaign on June 1st and 13th, 2021 (Soltwedel, 2021). The initial samples were obtained from a depth of 15 m using CTD-bound Niskin bottles (SBE 32 Carousel Water Sampler attached to a Seabird SBE911+ CTD system; Seabird Scientific, Bellevue, WA, USA) and, following filtration through a 150 µm mesh, the communities were incubated on plankton wheels within three temperature-controlled containers. To simulate current and potential future temperature scenarios in the Arctic Ocean, we selected a control temperature of 2 °C, an intermediate warming scenario of 6 °C, and a higher warming scenario of 9 °C while varying several other parameters such as light, nutrients and dilution level. Parameters were sampled at several timepoints throughout the two incubations. This dataset includes measurements of chlorophyll, particulate nutrients, dissolved nutrients, carbonate chemistry, and flow cytometry at several points of the incubations. For chlorophyll a analysis, 300 ml of sample water were filtered under vacuum (<−200 mbar) onto pre-combusted glass-fiber filters (GF/F Whatman, Maidstone, UK), while 200 ml were used for particulate organic carbon and nitrogen, with equal volumes of ultrapure water used for blank corrections. The filters were stored at −80 °C in 2 ml cryovials (Sarstedt, Nümbrecht, Germany) until analysis. Chlorophyll a filters were manually shredded in 6 ml of 90% acetone and extracted for 20 hours at 8 °C, following EPA method 445.0 (Arar and Collins, 1997). After centrifugation, chlorophyll a concentration was measured using a Trilogy fluorometer (Turner Designs, San Jose, CA, USA) with corrections for phaeopigments through acidification (1 M HCl). For particulate nutrient analysis, filters were acidified with 0.5 M HCl and dried at 60 °C for 12 hours, and the elemental composition was determined via gas chromatography using a CHNS-O elemental analyzer (EURO EA 3000, HEKAtech, Wegberg, Germany). pH was measured with an EcoScan pH 5 meter (ThermoFisher Scientific, Waltham, MA, USA) and a Sentix 62 glass electrode (Mettler Toledo, Columbus, OH, USA), calibrated using a pH 7 buffer solution (Mettler Toledo). Samples for total alkalinity and dissolved nutrients were filtered through a 0.22 µm cellulose-acetate syringe filter (Nalgene, Rochester, NY, USA) and stored at 4 °C in borosilicate and polycarbonate containers. Total alkalinity was determined by potentiometric titration using a TitroLine alphaplus autosampler (Schott Instruments, Mainz, Germany), with corrections applied using certified reference materials from A. Dickson (Scripps Institution of Oceanography, San Diego, CA, USA). The complete carbonate system was modeled using CO2sys (Pierrot et al., 2011), applying the dissociation constants of carbonic acid from Mehrbach et al. (1973), as refitted by Dickson and Millero (1987). Dissolved nutrient concentrations were analyzed using a continuous-flow autoanalyzer (Evolution III, Alliance Instruments, Freilassing, Germany), employing standard methods for nitrate and nitrite (Armstrong et al., 1967), phosphate (Eberlein and Kattner, 1987), silicate (Grasshoff et al., 2009), and ammonium (Koroleff, 1970). |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_973153 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Temperature incubations of microbial communities from two different locations in Fram Strait - bulk data Ahme, Antonia John, Uwe Alkalinity, total; Ammonium; Arctic; Bacteria; Bicarbonate ion; Bottle incubation in rotating plankton wheel in a light-controlled box; Calculated using CO2sys program with dissociation constants of carbonic acid by Mehrbach et al. (1973), refitted by Dickson and Millero (1987); Carbon, inorganic, total; Carbon, organic, particulate; Carbon dioxide; Carbon dioxide, partial pressure; Carbon trioxide; Changing Earth – Sustaining our Future; Chlorophyll a; CHNSO analyser, HEKAtech, Euro EA 3000; Continuous flow autoanalyzer, Alliance Instruments, Evolution III; CTD/Rosette; CTD-RO; CTD Rosette SBE 32; Date/time start, experiment; DEPTH, water; Dilution factor; East Greenland Current; Experiment; Fram Strait; GPF 20-1_021; Helmholtz_ChangingEarth; Incubation duration; incubation experiment; Laboratory experiment; Laboratory fluorometer, Turner Designs, Trilogy; Light intensity; Microplankton; Nitrate; Nitrite; Nitrogen, organic, particulate; North Greenland Sea; Pelagic microorganisms; pH; pH meter, Thermo Scientific, EcoScan pH 5; coupled with pH glass electrode, Mettler Toledo, Sentix 62; Phosphate; Phytoplankton; Polarstern; protists; PS126; PS126_20-6; PS126_3-3; Replicate; Sample ID; Sample source; Silicate; species composition; Station label; Temperature; Temperature, water; Titration analyzer, Schott Instruments, TitroLine alpha plus; corrected with certified reference (A. Dickson, Scripps Institution of Oceanography, San Diego, CA, USA); traits; Treatment: nitrate; Treatment: phosphate; Treatment: silicate; Treatment: vitamins + trace metals, added; Type of community; Type of study; Water description; West Spitsbergen Current This dataset encompasses data derived from two temperature incubation experiments aboard the RV Polarstern, using a unicellular microbial community collected from different stations in the Fram Strait during the PS126 campaign on June 1st and 13th, 2021 (Soltwedel, 2021). The initial samples were obtained from a depth of 15 m using CTD-bound Niskin bottles (SBE 32 Carousel Water Sampler attached to a Seabird SBE911+ CTD system; Seabird Scientific, Bellevue, WA, USA) and, following filtration through a 150 µm mesh, the communities were incubated on plankton wheels within three temperature-controlled containers. To simulate current and potential future temperature scenarios in the Arctic Ocean, we selected a control temperature of 2 °C, an intermediate warming scenario of 6 °C, and a higher warming scenario of 9 °C while varying several other parameters such as light, nutrients and dilution level. Parameters were sampled at several timepoints throughout the two incubations. This dataset includes measurements of chlorophyll, particulate nutrients, dissolved nutrients, carbonate chemistry, and flow cytometry at several points of the incubations. For chlorophyll a analysis, 300 ml of sample water were filtered under vacuum (<−200 mbar) onto pre-combusted glass-fiber filters (GF/F Whatman, Maidstone, UK), while 200 ml were used for particulate organic carbon and nitrogen, with equal volumes of ultrapure water used for blank corrections. The filters were stored at −80 °C in 2 ml cryovials (Sarstedt, Nümbrecht, Germany) until analysis. Chlorophyll a filters were manually shredded in 6 ml of 90% acetone and extracted for 20 hours at 8 °C, following EPA method 445.0 (Arar and Collins, 1997). After centrifugation, chlorophyll a concentration was measured using a Trilogy fluorometer (Turner Designs, San Jose, CA, USA) with corrections for phaeopigments through acidification (1 M HCl). For particulate nutrient analysis, filters were acidified with 0.5 M HCl and dried at 60 °C for 12 hours, and the elemental composition was determined via gas chromatography using a CHNS-O elemental analyzer (EURO EA 3000, HEKAtech, Wegberg, Germany). pH was measured with an EcoScan pH 5 meter (ThermoFisher Scientific, Waltham, MA, USA) and a Sentix 62 glass electrode (Mettler Toledo, Columbus, OH, USA), calibrated using a pH 7 buffer solution (Mettler Toledo). Samples for total alkalinity and dissolved nutrients were filtered through a 0.22 µm cellulose-acetate syringe filter (Nalgene, Rochester, NY, USA) and stored at 4 °C in borosilicate and polycarbonate containers. Total alkalinity was determined by potentiometric titration using a TitroLine alphaplus autosampler (Schott Instruments, Mainz, Germany), with corrections applied using certified reference materials from A. Dickson (Scripps Institution of Oceanography, San Diego, CA, USA). The complete carbonate system was modeled using CO2sys (Pierrot et al., 2011), applying the dissociation constants of carbonic acid from Mehrbach et al. (1973), as refitted by Dickson and Millero (1987). Dissolved nutrient concentrations were analyzed using a continuous-flow autoanalyzer (Evolution III, Alliance Instruments, Freilassing, Germany), employing standard methods for nitrate and nitrite (Armstrong et al., 1967), phosphate (Eberlein and Kattner, 1987), silicate (Grasshoff et al., 2009), and ammonium (Koroleff, 1970). |
| title | Temperature incubations of microbial communities from two different locations in Fram Strait - bulk data |
| topic | Alkalinity, total; Ammonium; Arctic; Bacteria; Bicarbonate ion; Bottle incubation in rotating plankton wheel in a light-controlled box; Calculated using CO2sys program with dissociation constants of carbonic acid by Mehrbach et al. (1973), refitted by Dickson and Millero (1987); Carbon, inorganic, total; Carbon, organic, particulate; Carbon dioxide; Carbon dioxide, partial pressure; Carbon trioxide; Changing Earth – Sustaining our Future; Chlorophyll a; CHNSO analyser, HEKAtech, Euro EA 3000; Continuous flow autoanalyzer, Alliance Instruments, Evolution III; CTD/Rosette; CTD-RO; CTD Rosette SBE 32; Date/time start, experiment; DEPTH, water; Dilution factor; East Greenland Current; Experiment; Fram Strait; GPF 20-1_021; Helmholtz_ChangingEarth; Incubation duration; incubation experiment; Laboratory experiment; Laboratory fluorometer, Turner Designs, Trilogy; Light intensity; Microplankton; Nitrate; Nitrite; Nitrogen, organic, particulate; North Greenland Sea; Pelagic microorganisms; pH; pH meter, Thermo Scientific, EcoScan pH 5; coupled with pH glass electrode, Mettler Toledo, Sentix 62; Phosphate; Phytoplankton; Polarstern; protists; PS126; PS126_20-6; PS126_3-3; Replicate; Sample ID; Sample source; Silicate; species composition; Station label; Temperature; Temperature, water; Titration analyzer, Schott Instruments, TitroLine alpha plus; corrected with certified reference (A. Dickson, Scripps Institution of Oceanography, San Diego, CA, USA); traits; Treatment: nitrate; Treatment: phosphate; Treatment: silicate; Treatment: vitamins + trace metals, added; Type of community; Type of study; Water description; West Spitsbergen Current |
| url | https://doi.org/10.1594/PANGAEA.973153 |