Amphipod glycogen and glucose content under simulated invasion stress in wetlands
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2024
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| _version_ | 1867168530505400320 |
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| author | Tanner, Richelle L Haworth, Lorna E Nancollas, Sarah Landa, Susie N Todgham, Anne E |
| author_facet | Tanner, Richelle L Haworth, Lorna E Nancollas, Sarah Landa, Susie N Todgham, Anne E |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Every two weeks during the 6-week acclimation period, 8 adults were randomly selected from each tank glycogen analysis (n = 8). Individuals were sacrificed in dry ice and stored at -80 °C. Frozen amphipods were ground into fine powder using a liquid nitrogen cooled mortar and pestle. Glucose and glycogen metabolites were extracted separately to be analyzed using a colorimetric assay. Glycogen was extracted by adding 1 ml of ice-cold 8% HClO₄ to approximately 20 mg of powdered amphipod tissue and then homogenized on ice for approximately 10 seconds with a Pro200 Bio-Gen Series homogenizer (PROScientific, Oxford, CT, USA). A 200 μl sample of the homogenate was frozen at -80 °C for later glycogen analysis. The remaining homogenate slurry was centrifuged at 10,000 g for 10 minutes at 4 °C and the supernatant was extracted and neutralized with 3 mol l⁻¹ K₂CO₃. The solution was centrifuged again at 10,000 g for 10 minutes at 4 °C and frozen at -80 °C for the glucose assay. Glycogen samples were enzymatically digested using previous methods (Hassid & Abraham, 1957) and analyzed for glucose following methodology (Bergmeyer, 1983) modified for microplate spectrophotometer (Synergy HT, Biotek, Winooski, VT, USA). Glycogen content was then corrected for starting glucose. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_973189 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Amphipod glycogen and glucose content under simulated invasion stress in wetlands Tanner, Richelle L Haworth, Lorna E Nancollas, Sarah Landa, Susie N Todgham, Anne E Amphipoda; Controlled using microcontroller board, Uno WIFI, Arduino; Experiment week; Glucose, per wet mass; Glycogen, per wet mass; HAND; invasion ecology; Laboratory experiment; Microplate Spectrophotometer, TECAN, Spark; Origin of Gammarus sp. for the experiments; physiology; Replicate; Sampling by hand; Sampling date/time, experiment; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Species identification; Suisun Marsh, California, United States; Tank number; Treatment: light condition; Treatment: temperature description; TuleRed; Type of study; VID; Visual identification; wetland Every two weeks during the 6-week acclimation period, 8 adults were randomly selected from each tank glycogen analysis (n = 8). Individuals were sacrificed in dry ice and stored at -80 °C. Frozen amphipods were ground into fine powder using a liquid nitrogen cooled mortar and pestle. Glucose and glycogen metabolites were extracted separately to be analyzed using a colorimetric assay. Glycogen was extracted by adding 1 ml of ice-cold 8% HClO₄ to approximately 20 mg of powdered amphipod tissue and then homogenized on ice for approximately 10 seconds with a Pro200 Bio-Gen Series homogenizer (PROScientific, Oxford, CT, USA). A 200 μl sample of the homogenate was frozen at -80 °C for later glycogen analysis. The remaining homogenate slurry was centrifuged at 10,000 g for 10 minutes at 4 °C and the supernatant was extracted and neutralized with 3 mol l⁻¹ K₂CO₃. The solution was centrifuged again at 10,000 g for 10 minutes at 4 °C and frozen at -80 °C for the glucose assay. Glycogen samples were enzymatically digested using previous methods (Hassid & Abraham, 1957) and analyzed for glucose following methodology (Bergmeyer, 1983) modified for microplate spectrophotometer (Synergy HT, Biotek, Winooski, VT, USA). Glycogen content was then corrected for starting glucose. |
| title | Amphipod glycogen and glucose content under simulated invasion stress in wetlands |
| topic | Amphipoda; Controlled using microcontroller board, Uno WIFI, Arduino; Experiment week; Glucose, per wet mass; Glycogen, per wet mass; HAND; invasion ecology; Laboratory experiment; Microplate Spectrophotometer, TECAN, Spark; Origin of Gammarus sp. for the experiments; physiology; Replicate; Sampling by hand; Sampling date/time, experiment; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Species identification; Suisun Marsh, California, United States; Tank number; Treatment: light condition; Treatment: temperature description; TuleRed; Type of study; VID; Visual identification; wetland |
| url | https://doi.org/10.1594/PANGAEA.973189 |