Cytotype-level responses and of Spartina plants to drought and elevated atmospheric CO₂ concentration
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2025
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| _version_ | 1867170127523348480 |
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| author | Granse, Dirk Wendt, Paul Suchrow, Sigrid Hanelt, Dieter Fromm, Jörg Milin, Morgane Lima, Oscar Salmon, Armel Ainouche, Malika Jensen, Kai |
| author_facet | Granse, Dirk Wendt, Paul Suchrow, Sigrid Hanelt, Dieter Fromm, Jörg Milin, Morgane Lima, Oscar Salmon, Armel Ainouche, Malika Jensen, Kai |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Global change factors, such as drought and elevated atmospheric CO₂ levels, can have strong impacts on plant performance and evolutionary development. We investigated responses of Spartina (syn.: Sporobolus P.M.Peterson & Saarela) plants on different ploidy levels (cytotypes), i.e., the hybrid Spartina x townsendii H. Groves & J. Groves (syn.: Sporobolus × townsendii P.M.Peterson & Saarela) and its genome-duplicated (allopolyploid) descendant Spartina anglica C.E. Hubbard (syn.: Sporobolus anglicus P.M.Peterson & Saarela), to drought and elevated atmospheric CO₂ levels, regarding plant functional traits and biomass. Spartina plants were sampled in 2017 from a Spartina-marsh at Sönke-Nissen-Koog, Germany, and cytotype analysis was performed by DAPI staining for flow cytometry. Ramets from donor individuals of these plants were used in a greenhouse experiment at University of Hamburg, Germany. The plants were grown in 2 L pots and weekly fertilized with commercial Hakaphos® Blau (15-10-15) NPK fertilizer solution (1% v/v; 50 mL per pot). Three cytotype replicates of both hybrid and allopolyploid plants were treated from May to October 2018 under greenhouse daylight conditions (day/night air temperature 27 °C / 22 °C; 70%–80% relative air humidity; length of the photoperiod typical for the northern Germany region) with drought (vs. well-watered) and elevated (vs. ambient) CO₂ concentrations. At the end of the experiment, total biomass and plant traits, i.e., root-to-shoot ratio, stem diameter, leaf area, stem density, and stomatal length were determined. We here report the result values of three to six technical replicates from three biological replicates per treatment combination. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_973270 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Cytotype-level responses and of Spartina plants to drought and elevated atmospheric CO₂ concentration Granse, Dirk Wendt, Paul Suchrow, Sigrid Hanelt, Dieter Fromm, Jörg Milin, Morgane Lima, Oscar Salmon, Armel Ainouche, Malika Jensen, Kai Analytical balance, Sartorius, A 120 S; Biomass, total; Calculated; Caliper; Counting; Cytotype; Event label; Flow cytometer, Partec GmbH, PA-II; Greenhouse experiment; Hybrids; Hybrids - Chances and Challenges of New Genetic Combinations; Individual code; Leaf area; Light microscope, Olympus, BH-2; coupled with Microscope camera, Bresser, MikroCam SP 5.0; Replicate; Root/shoot ratio; RULER; Ruler stick; Sample number; Sampling date/time, experiment; SHS2017; SOILS; Soil sample; Species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Stem density, per pot; Stem diameter; Stem height; Stomatal length; Treatment: carbon dioxide; Treatment: water; Type of study; Wadden Sea Global change factors, such as drought and elevated atmospheric CO₂ levels, can have strong impacts on plant performance and evolutionary development. We investigated responses of Spartina (syn.: Sporobolus P.M.Peterson & Saarela) plants on different ploidy levels (cytotypes), i.e., the hybrid Spartina x townsendii H. Groves & J. Groves (syn.: Sporobolus × townsendii P.M.Peterson & Saarela) and its genome-duplicated (allopolyploid) descendant Spartina anglica C.E. Hubbard (syn.: Sporobolus anglicus P.M.Peterson & Saarela), to drought and elevated atmospheric CO₂ levels, regarding plant functional traits and biomass. Spartina plants were sampled in 2017 from a Spartina-marsh at Sönke-Nissen-Koog, Germany, and cytotype analysis was performed by DAPI staining for flow cytometry. Ramets from donor individuals of these plants were used in a greenhouse experiment at University of Hamburg, Germany. The plants were grown in 2 L pots and weekly fertilized with commercial Hakaphos® Blau (15-10-15) NPK fertilizer solution (1% v/v; 50 mL per pot). Three cytotype replicates of both hybrid and allopolyploid plants were treated from May to October 2018 under greenhouse daylight conditions (day/night air temperature 27 °C / 22 °C; 70%–80% relative air humidity; length of the photoperiod typical for the northern Germany region) with drought (vs. well-watered) and elevated (vs. ambient) CO₂ concentrations. At the end of the experiment, total biomass and plant traits, i.e., root-to-shoot ratio, stem diameter, leaf area, stem density, and stomatal length were determined. We here report the result values of three to six technical replicates from three biological replicates per treatment combination. |
| title | Cytotype-level responses and of Spartina plants to drought and elevated atmospheric CO₂ concentration |
| topic | Analytical balance, Sartorius, A 120 S; Biomass, total; Calculated; Caliper; Counting; Cytotype; Event label; Flow cytometer, Partec GmbH, PA-II; Greenhouse experiment; Hybrids; Hybrids - Chances and Challenges of New Genetic Combinations; Individual code; Leaf area; Light microscope, Olympus, BH-2; coupled with Microscope camera, Bresser, MikroCam SP 5.0; Replicate; Root/shoot ratio; RULER; Ruler stick; Sample number; Sampling date/time, experiment; SHS2017; SOILS; Soil sample; Species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Stem density, per pot; Stem diameter; Stem height; Stomatal length; Treatment: carbon dioxide; Treatment: water; Type of study; Wadden Sea |
| url | https://doi.org/10.1594/PANGAEA.973270 |