Total bacteria and archaea number with different nematodes and substrates conditions
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2025
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| _version_ | 1867169163836915712 |
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| author | Tamang, Mandip Huber, Katharina Neumann-Schaal, Meina Pester, Michael |
| author_facet | Tamang, Mandip Huber, Katharina Neumann-Schaal, Meina Pester, Michael |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Data contain the quantitative polymerase chain reaction (qPCR) numbers of total bacterial and archaeal 16S ribosomal RNA (16S rRNA) genes from 3 different nematode treatments and two different substrates conditions in the natural soil. In first treatment condition there was no addition of nematode, in second treatment bacterivorous nematode (Acrobeloides buetschlii) was added and in third treatment there was the addition of fungivorous nematode (Aphelenchoides saprophilus). Meanwhile, two different substrate conditions were: with and without maize litter. The natural soil for these experiments were collected from Dikopshof agricultural site (50°48′21″ N, 6°59′9″ E), Bonn, Germany. These qPCR numbers were collected in June and July, 2023 to look at the effect of nematode grazing in the bacterial and archaeal populations. The qPCR was performed using primer set of 1389F (5′-TGYACACACCGCCCGT-3′) and 1492R (5′-GGYTACCTTGTTACGACTT-3′) with 20 µl reactions on a qTOWER3G (Analytik-Jena, Jena, Germany). |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_974253 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Total bacteria and archaea number with different nematodes and substrates conditions Tamang, Mandip Huber, Katharina Neumann-Schaal, Meina Pester, Michael Dikopshof_agricultural_site; Experimental treatment; Gene copies per gram sediment; Laboratory experiment; Nematode grazing; quantitative PCR; Sample code/label; Treatment: duration; Treatment: grazers; Trophic interactions Data contain the quantitative polymerase chain reaction (qPCR) numbers of total bacterial and archaeal 16S ribosomal RNA (16S rRNA) genes from 3 different nematode treatments and two different substrates conditions in the natural soil. In first treatment condition there was no addition of nematode, in second treatment bacterivorous nematode (Acrobeloides buetschlii) was added and in third treatment there was the addition of fungivorous nematode (Aphelenchoides saprophilus). Meanwhile, two different substrate conditions were: with and without maize litter. The natural soil for these experiments were collected from Dikopshof agricultural site (50°48′21″ N, 6°59′9″ E), Bonn, Germany. These qPCR numbers were collected in June and July, 2023 to look at the effect of nematode grazing in the bacterial and archaeal populations. The qPCR was performed using primer set of 1389F (5′-TGYACACACCGCCCGT-3′) and 1492R (5′-GGYTACCTTGTTACGACTT-3′) with 20 µl reactions on a qTOWER3G (Analytik-Jena, Jena, Germany). |
| title | Total bacteria and archaea number with different nematodes and substrates conditions |
| topic | Dikopshof_agricultural_site; Experimental treatment; Gene copies per gram sediment; Laboratory experiment; Nematode grazing; quantitative PCR; Sample code/label; Treatment: duration; Treatment: grazers; Trophic interactions |
| url | https://doi.org/10.1594/PANGAEA.974253 |