Incubation Experiments of DOM with Prokaryotic Community at the NW Mediterranean Sea site MOLA in 2021
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
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PANGAEA
2026
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| _version_ | 1867171046983991296 |
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| author | Bouchachi, Nawal Campos, Rebeca Crispi, Olivier Marie, Barbara Paulin, Charles-Hubert Escoubeyrou, Karine Colombet, Jonathan Sime-Ngando, Telesphore Ortega-Retuerta, Eva |
| author_facet | Bouchachi, Nawal Campos, Rebeca Crispi, Olivier Marie, Barbara Paulin, Charles-Hubert Escoubeyrou, Karine Colombet, Jonathan Sime-Ngando, Telesphore Ortega-Retuerta, Eva |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The water samples for the incubation experiment were collected on the RV Néréïs II at the Microbial Observatory Laboratory Arago 42N and 4E on 31 May and 26 August 2021. The parameters include DOC/TDN/DON/TDP/DOP, dissolved hydrolyzable amino acids, fluorescent dissolved organic matter (FDOM), enzyme activity, prokaryote heterotrophic production (PHP), prokaryote and viral abundance. The incubation temperature was temperature +13ºC for both experiments. DOC/TDN was analyzed using High-Temperature Catalytic Oxidation (TOC-VCSH). DIN/TDP/DOP was analyzed using a Technicon Auto Analyzer. Dissolved hydrolyzable amino acids were analyzed using High Performal Liquid Chromatography (HPLC). FDOM was measured on a FP-8500 Spectrofluorimeter (Jasco, Japan). Enzymatic activities were measured using 4-methylumbelliferyl-butyrate (esterase), 4-methylumbelliferyl phosphate for alkaline phosphatase (APase), and L-leucine-7-amido-4-methyl coumarin for leu-aminopeptidase (AMA). PHP was measured using 3h-leucine and the microcentrifuge method. Prokaryote and viral abundance were measured on a flow cytometer (Cytoflex, Beckman Coulter, USA). The purpose of data collection was to understand the seasonal cycling of organic matter and heterotrophic bacteria dynamics within the microbial loop. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_974747 |
| institution | PANGAEA |
| language | en |
| publishDate | 2026 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Incubation Experiments of DOM with Prokaryotic Community at the NW Mediterranean Sea site MOLA in 2021 Bouchachi, Nawal Campos, Rebeca Crispi, Olivier Marie, Barbara Paulin, Charles-Hubert Escoubeyrou, Karine Colombet, Jonathan Sime-Ngando, Telesphore Ortega-Retuerta, Eva 3H-leucine microcentrifuge; Alkaline phosphatase activity; Ammonium; Auto-analyzer, Technicon AA-II; Bacteria; beta-Alanine; Bottle, Niskin; Calculated; Carbon, organic, dissolved; D-Alanine; D-Aspartic acid; Date/time analysis, experiment; Depth, sampling; D-Glutamic acid; D-Leucine; DOM; D-Serine; Esterase activity; Excitation Emission Spectrofluorometer, 260 nm/450 nm, JASCO FP-8500; experimental data; FACSCalibur flow-cytometer (Becton Dickinson); Flow cytometer, Beckman Coulter, CytoFLEX; Fluorescence, dissolved organic matter, A coble-peak; Fluorescence, dissolved organic matter, B coble-peak; Fluorescence, dissolved organic matter, C coble-peak; Fluorescence, dissolved organic matter, M coble-peak; Fluorescence, dissolved organic matter, T coble-peak; gamma-Aminobutyric acid; Glycine; High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000; High temperature catalytic oxidation (TOC-VCSH); L-Alanine; L-Arginine; L-Aspartic acid; Leucine aminopeptidase activity; Leucine incorporation rate; L-Glutamic acid; L-Histidine; L-Leucine; L-Lysine; L-Phenylalanine; L-Serine; L-Threonine; L-Tyrosine; L-Valine; Mediterranean Sea; Methionine; MOLA_Aug2021; MOLA_May2021; Monitoring the impact of climate change on hydrology and biogeochemical cycles in the Gulf of Lion (MOLA); MOOSE_MOLA_2021; MOOSE (MOLA) 2021; MOOSE-MOLA; Nereis II; NIS; Nitrogen, inorganic, dissolved; Nitrogen, organic, dissolved; Nitrogen, total dissolved; Phosphorus, inorganic, dissolved; Phosphorus, organic, dissolved; Phosphorus, total dissolved; Replicate; Sample code/label; Virus, high DNA fluorescence; Virus, low DNA fluorescence The water samples for the incubation experiment were collected on the RV Néréïs II at the Microbial Observatory Laboratory Arago 42N and 4E on 31 May and 26 August 2021. The parameters include DOC/TDN/DON/TDP/DOP, dissolved hydrolyzable amino acids, fluorescent dissolved organic matter (FDOM), enzyme activity, prokaryote heterotrophic production (PHP), prokaryote and viral abundance. The incubation temperature was temperature +13ºC for both experiments. DOC/TDN was analyzed using High-Temperature Catalytic Oxidation (TOC-VCSH). DIN/TDP/DOP was analyzed using a Technicon Auto Analyzer. Dissolved hydrolyzable amino acids were analyzed using High Performal Liquid Chromatography (HPLC). FDOM was measured on a FP-8500 Spectrofluorimeter (Jasco, Japan). Enzymatic activities were measured using 4-methylumbelliferyl-butyrate (esterase), 4-methylumbelliferyl phosphate for alkaline phosphatase (APase), and L-leucine-7-amido-4-methyl coumarin for leu-aminopeptidase (AMA). PHP was measured using 3h-leucine and the microcentrifuge method. Prokaryote and viral abundance were measured on a flow cytometer (Cytoflex, Beckman Coulter, USA). The purpose of data collection was to understand the seasonal cycling of organic matter and heterotrophic bacteria dynamics within the microbial loop. |
| title | Incubation Experiments of DOM with Prokaryotic Community at the NW Mediterranean Sea site MOLA in 2021 |
| topic | 3H-leucine microcentrifuge; Alkaline phosphatase activity; Ammonium; Auto-analyzer, Technicon AA-II; Bacteria; beta-Alanine; Bottle, Niskin; Calculated; Carbon, organic, dissolved; D-Alanine; D-Aspartic acid; Date/time analysis, experiment; Depth, sampling; D-Glutamic acid; D-Leucine; DOM; D-Serine; Esterase activity; Excitation Emission Spectrofluorometer, 260 nm/450 nm, JASCO FP-8500; experimental data; FACSCalibur flow-cytometer (Becton Dickinson); Flow cytometer, Beckman Coulter, CytoFLEX; Fluorescence, dissolved organic matter, A coble-peak; Fluorescence, dissolved organic matter, B coble-peak; Fluorescence, dissolved organic matter, C coble-peak; Fluorescence, dissolved organic matter, M coble-peak; Fluorescence, dissolved organic matter, T coble-peak; gamma-Aminobutyric acid; Glycine; High Performance Liquid Chromatography (HPLC), ortho-phthaldialdehyde derivatization, UltiMate 3000; High temperature catalytic oxidation (TOC-VCSH); L-Alanine; L-Arginine; L-Aspartic acid; Leucine aminopeptidase activity; Leucine incorporation rate; L-Glutamic acid; L-Histidine; L-Leucine; L-Lysine; L-Phenylalanine; L-Serine; L-Threonine; L-Tyrosine; L-Valine; Mediterranean Sea; Methionine; MOLA_Aug2021; MOLA_May2021; Monitoring the impact of climate change on hydrology and biogeochemical cycles in the Gulf of Lion (MOLA); MOOSE_MOLA_2021; MOOSE (MOLA) 2021; MOOSE-MOLA; Nereis II; NIS; Nitrogen, inorganic, dissolved; Nitrogen, organic, dissolved; Nitrogen, total dissolved; Phosphorus, inorganic, dissolved; Phosphorus, organic, dissolved; Phosphorus, total dissolved; Replicate; Sample code/label; Virus, high DNA fluorescence; Virus, low DNA fluorescence |
| url | https://doi.org/10.1594/PANGAEA.974747 |