Chlorophyll-a per algal cell of coral Xenia umbellata in a aquarium experiment
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| Auteurs principaux: | , , , , , , , , |
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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2025
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| _version_ | 1867171340174229504 |
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| author | Hill, Claudia E L Rücker, Julia Eichhorn, Nele Mezger, Selma D Caporale, Giulia El-Khaled, Yusuf C Blanco, Sahara Wild, Christian Tilstra, Arjen |
| author_facet | Hill, Claudia E L Rücker, Julia Eichhorn, Nele Mezger, Selma D Caporale, Giulia El-Khaled, Yusuf C Blanco, Sahara Wild, Christian Tilstra, Arjen |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | One colony per tank was randomly selected every 7 days and stored at -20°C. On analysis day, colonies were thawed, homogenised in 10 ml demineralised water, and the slurry volume was measured. 2ml of the slurry was aliquoted, centrifuged twice to separate algal cells from coral tissue, and resuspended in demineralised water. In a darkened room, pellets were resuspended in 2 ml of 90% acetone, vortexed, and stored at 4°C for 24 h. After centrifugation, samples were split into two 1 ml cuvettes and measured on a UV-Spectrophotometer (663 nm & 630 nm) against a calibration curve. Each subsample was measured three times, with readings normalised to slurry volume and Symbiodiniaceae cell count. Xenia umbellata originates from the Red Sea. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_974805 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Chlorophyll-a per algal cell of coral Xenia umbellata in a aquarium experiment Hill, Claudia E L Rücker, Julia Eichhorn, Nele Mezger, Selma D Caporale, Giulia El-Khaled, Yusuf C Blanco, Sahara Wild, Christian Tilstra, Arjen Algae, chlorophyll a per cell; Day of experiment; Laboratory experiment; Tank number; Treatment; Type of study; V-Spectrophotometer, Fisher Scientific, Germany [GENESYS 150] One colony per tank was randomly selected every 7 days and stored at -20°C. On analysis day, colonies were thawed, homogenised in 10 ml demineralised water, and the slurry volume was measured. 2ml of the slurry was aliquoted, centrifuged twice to separate algal cells from coral tissue, and resuspended in demineralised water. In a darkened room, pellets were resuspended in 2 ml of 90% acetone, vortexed, and stored at 4°C for 24 h. After centrifugation, samples were split into two 1 ml cuvettes and measured on a UV-Spectrophotometer (663 nm & 630 nm) against a calibration curve. Each subsample was measured three times, with readings normalised to slurry volume and Symbiodiniaceae cell count. Xenia umbellata originates from the Red Sea. |
| title | Chlorophyll-a per algal cell of coral Xenia umbellata in a aquarium experiment |
| topic | Algae, chlorophyll a per cell; Day of experiment; Laboratory experiment; Tank number; Treatment; Type of study; V-Spectrophotometer, Fisher Scientific, Germany [GENESYS 150] |
| url | https://doi.org/10.1594/PANGAEA.974805 |