| _version_ | 1867170129346822144 |
|---|---|
| author | Stief, Peter |
| author_facet | Stief, Peter |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Four diatom strains obtained from different culture collections were grown to stationary phase in L1 medium plus silicate (Guillard & Hargraves 1993) at 15°C under a light/dark regime of 14/10 h. For experimentation, the diatom strains were incubated at either atmospheric pressure (0.1 MPa = control treatment) or at gradually increasing hydrostatic pressure (0.1-100 MPa = pressure treatment) throughout 21 days in darkness and at 3°C. The pressure treatment simulated the sinking of particle-associated diatoms from the ocean surface down into a hadal trench of 10 km depth. Pressure-induced leakage of dissolved organic matter (DOM) was followed throughout the incubations. Both ambient concentrations and intracellular concentrations of different DOM components were measured: Dissolved organic carbon (DOC), total dissolved nitrogen (TDN), protein-like and humic-like DOM fluorescence, total proteins, total polysaccharides, laminarin, and dimethylsulfoniopropionate (DMSP). The cell abundance of the different diatom strains was followed throughout the incubations. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_974877 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Pressure-induced leakage of dissolved organic matter (DOM) from different diatom species Stief, Peter Carbon, organic, dissolved; Carbon, organic, dissolved, intracellular; culture experiment; Date/time end, experiment; Date/time start, experiment; Diatom; Diatoms; Dimethylsulfoniopropionate; Dimethylsulfoniopropionate, intracellular; DOM; Fluorescence, humic-like, component; Fluorescence, protein-like, component; Fluorescence spectrometer, AquaLog, HORIBA JobinYvon; Gas chromatography; hydrostatic pressure; Laboratory experiment; Laminarin; Laminarin, intracellular; Laminarinase-based analysis (Becker et al. 2017); leakage; Light microscope; Lowry assay after Lowry et al. 1951; marine snow; Nitrogen, total dissolved; Nitrogen, total dissolved, intracellular; PAHBAH reducing sugar assay (Lever 1972); Polysaccharides; Polysaccharides, intracellular; Proteins, total; Proteins, total, intracellular; Sample code/label; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Total organic carbon analyzer, Shimadzu, TOC-L; Treatment: duration; Treatment: pressure; Type of study Four diatom strains obtained from different culture collections were grown to stationary phase in L1 medium plus silicate (Guillard & Hargraves 1993) at 15°C under a light/dark regime of 14/10 h. For experimentation, the diatom strains were incubated at either atmospheric pressure (0.1 MPa = control treatment) or at gradually increasing hydrostatic pressure (0.1-100 MPa = pressure treatment) throughout 21 days in darkness and at 3°C. The pressure treatment simulated the sinking of particle-associated diatoms from the ocean surface down into a hadal trench of 10 km depth. Pressure-induced leakage of dissolved organic matter (DOM) was followed throughout the incubations. Both ambient concentrations and intracellular concentrations of different DOM components were measured: Dissolved organic carbon (DOC), total dissolved nitrogen (TDN), protein-like and humic-like DOM fluorescence, total proteins, total polysaccharides, laminarin, and dimethylsulfoniopropionate (DMSP). The cell abundance of the different diatom strains was followed throughout the incubations. |
| title | Pressure-induced leakage of dissolved organic matter (DOM) from different diatom species |
| topic | Carbon, organic, dissolved; Carbon, organic, dissolved, intracellular; culture experiment; Date/time end, experiment; Date/time start, experiment; Diatom; Diatoms; Dimethylsulfoniopropionate; Dimethylsulfoniopropionate, intracellular; DOM; Fluorescence, humic-like, component; Fluorescence, protein-like, component; Fluorescence spectrometer, AquaLog, HORIBA JobinYvon; Gas chromatography; hydrostatic pressure; Laboratory experiment; Laminarin; Laminarin, intracellular; Laminarinase-based analysis (Becker et al. 2017); leakage; Light microscope; Lowry assay after Lowry et al. 1951; marine snow; Nitrogen, total dissolved; Nitrogen, total dissolved, intracellular; PAHBAH reducing sugar assay (Lever 1972); Polysaccharides; Polysaccharides, intracellular; Proteins, total; Proteins, total, intracellular; Sample code/label; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Total organic carbon analyzer, Shimadzu, TOC-L; Treatment: duration; Treatment: pressure; Type of study |
| url | https://doi.org/10.1594/PANGAEA.974877 |