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author Stief, Peter
author_facet Stief, Peter
collection Datos científicos de ciencias marinas y ambientales
contents Model diatom aggregates were produced from the diatom Skeletonema marinoi and the natural microbial community of seawater collected in Oeresund (Stief et al. 2023), Central Arctic Ocean, and Japan Trench. S. marinoi was grown to stationary phase in L1 medium plus silicate (Guillard & Hargraves 1993) at 15°C under a light/dark regime of 14/10 h. The diatom aggregates were individually incubated at either atmospheric pressure (0.1 MPa = control treatment) or at gradually increasing hydrostatic pressure (0.1-100 MPa = pressure treatment) throughout 20 days in darkness and at 3°C. The pressure treatment simulated the sinking of diatom-bacteria aggregates from the ocean surface down into a hadal trench of 10 km depth. Pressure-induced leakage of dissolved organic matter (DOM) was followed throughout the incubations. Ambient concentrations of different DOM components were measured: Dissolved organic carbon (DOC), total dissolved nitrogen (TDN), protein-like and humic-like DOM fluorescence.
format Dataset Open Access
id pangaea_https___doi_org_10_1594_PANGAEA_974878
institution PANGAEA
language en
publishDate 2025
publisher PANGAEA
record_format pangaea
spellingShingle Pressure-induced leakage of dissolved organic matter (DOM) from diatom-bacteria aggregates
Stief, Peter
ArcWatch-1; Carbon, organic, dissolved; CTD/Rosette; CTD-RO; culture experiment; Date/time end, experiment; Date/time start, experiment; diatoms; DOM; Event label; Fluorescence, humic-like, component; Fluorescence, protein-like, component; Fluorescence spectrometer, AquaLog, HORIBA JobinYvon; hydrostatic pressure; Laboratory experiment; leakage; marine snow; Nitrogen, total dissolved; Polarstern; PS138; PS138_218-1; Sample code/label; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); SW_072021; SW_102021; SW_102022; Total organic carbon analyzer, Shimadzu, TOC-L; Treatment: duration; Treatment: pressure; Type of study; UM23-06; UM23-06_173.5; Umitaka Maru
Model diatom aggregates were produced from the diatom Skeletonema marinoi and the natural microbial community of seawater collected in Oeresund (Stief et al. 2023), Central Arctic Ocean, and Japan Trench. S. marinoi was grown to stationary phase in L1 medium plus silicate (Guillard & Hargraves 1993) at 15°C under a light/dark regime of 14/10 h. The diatom aggregates were individually incubated at either atmospheric pressure (0.1 MPa = control treatment) or at gradually increasing hydrostatic pressure (0.1-100 MPa = pressure treatment) throughout 20 days in darkness and at 3°C. The pressure treatment simulated the sinking of diatom-bacteria aggregates from the ocean surface down into a hadal trench of 10 km depth. Pressure-induced leakage of dissolved organic matter (DOM) was followed throughout the incubations. Ambient concentrations of different DOM components were measured: Dissolved organic carbon (DOC), total dissolved nitrogen (TDN), protein-like and humic-like DOM fluorescence.
title Pressure-induced leakage of dissolved organic matter (DOM) from diatom-bacteria aggregates
topic ArcWatch-1; Carbon, organic, dissolved; CTD/Rosette; CTD-RO; culture experiment; Date/time end, experiment; Date/time start, experiment; diatoms; DOM; Event label; Fluorescence, humic-like, component; Fluorescence, protein-like, component; Fluorescence spectrometer, AquaLog, HORIBA JobinYvon; hydrostatic pressure; Laboratory experiment; leakage; marine snow; Nitrogen, total dissolved; Polarstern; PS138; PS138_218-1; Sample code/label; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); SW_072021; SW_102021; SW_102022; Total organic carbon analyzer, Shimadzu, TOC-L; Treatment: duration; Treatment: pressure; Type of study; UM23-06; UM23-06_173.5; Umitaka Maru
url https://doi.org/10.1594/PANGAEA.974878