Degradation of diatom-derived dissolved organic matter (DOM) in surface seawater
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
| Publicado: |
PANGAEA
2025
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| _version_ | 1867171893961818112 |
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| author | Stief, Peter |
| author_facet | Stief, Peter |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Stored surface seawater originally collected in the North Sea was amended with dissolved organic matter (DOM) obtained from the diatom Skeletonema marinoi by exposing the diatom culture to a hydrostatic pressure level of 40 MPa for 24 h. To this end, S. marinoi had been grown to stationary phase in L1 medium plus silicate (Guillard & Hargraves 1993) at 15°C under a light/dark regime of 14/10 h. Diatom-cell-free DOM was added to aliquoted seawater samples at initial concentrations of 250 µmol C/L. The microbial degradation of the added DOM and the microbial response to DOM-amendment at 15°C and in darkness was followed for 2 weeks. The seawater was subsampled at defined time intervals to analyze a number of variables. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_974879 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Degradation of diatom-derived dissolved organic matter (DOM) in surface seawater Stief, Peter Bacteria; Carbon, organic, dissolved; CTD/Rosette; CTD-RO; culture experiment; Date/time end, experiment; Date/time start, experiment; diatoms; DOM; DOM degradation; Fluorescence determination of DAPI stain; hydrostatic pressure; Laboratory experiment; leakage; Lowry assay after Lowry et al. 1951; microbial community; Nitrogen, total dissolved; Oxygen logger, FireSting, Pyroscience; PAHBAH reducing sugar assay (Lever 1972); Polysaccharides; Proteins, total; Respiration rate, oxygen; seawater; Species, unique identification; SW_042019; Total organic carbon analyzer, Shimadzu, TOC-L; Treatment: carbon; Treatment: duration; Type of study Stored surface seawater originally collected in the North Sea was amended with dissolved organic matter (DOM) obtained from the diatom Skeletonema marinoi by exposing the diatom culture to a hydrostatic pressure level of 40 MPa for 24 h. To this end, S. marinoi had been grown to stationary phase in L1 medium plus silicate (Guillard & Hargraves 1993) at 15°C under a light/dark regime of 14/10 h. Diatom-cell-free DOM was added to aliquoted seawater samples at initial concentrations of 250 µmol C/L. The microbial degradation of the added DOM and the microbial response to DOM-amendment at 15°C and in darkness was followed for 2 weeks. The seawater was subsampled at defined time intervals to analyze a number of variables. |
| title | Degradation of diatom-derived dissolved organic matter (DOM) in surface seawater |
| topic | Bacteria; Carbon, organic, dissolved; CTD/Rosette; CTD-RO; culture experiment; Date/time end, experiment; Date/time start, experiment; diatoms; DOM; DOM degradation; Fluorescence determination of DAPI stain; hydrostatic pressure; Laboratory experiment; leakage; Lowry assay after Lowry et al. 1951; microbial community; Nitrogen, total dissolved; Oxygen logger, FireSting, Pyroscience; PAHBAH reducing sugar assay (Lever 1972); Polysaccharides; Proteins, total; Respiration rate, oxygen; seawater; Species, unique identification; SW_042019; Total organic carbon analyzer, Shimadzu, TOC-L; Treatment: carbon; Treatment: duration; Type of study |
| url | https://doi.org/10.1594/PANGAEA.974879 |