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| Autores principales: | , , , , , , |
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
| Publicado: |
PANGAEA
2025
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| Materias: | |
| Acceso en línea: | https://doi.org/10.1594/PANGAEA.974989 |
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| _version_ | 1867171894195650560 |
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| author | Lee, Shu-An Low, Yee-Lynn Adhikari, Atin Wang, Wei-Kuang Liang, Chih-Ming Chang, Li-Te Tsai, Chuen-Jinn |
| author_facet | Lee, Shu-An Low, Yee-Lynn Adhikari, Atin Wang, Wei-Kuang Liang, Chih-Ming Chang, Li-Te Tsai, Chuen-Jinn |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Viability, culturability and DNA loss rate were obtained for collected bacteria after filter and impingement-based sampling. The sample extracts were spread on TSA plates. The inoculated TSA plates were incubated at 37℃ for 1 day, and colony forming units (CFUs) were counted for culturable fungi. Dual-fluorescence method for accurate viability analysis of test samples was performed. With an appropriate mixture of AO/PI, total cells were stained with AO whereas damaged cells were stained with PI. An epifluorescence microscope was used to count the test sample's bacterial cells on the filter at a magnification of 400x. The culturability is determined by percentage of CCFU in CAO while percentage of (CAO-CPI) in CAO is for viability. The qPCR was performed on LightCycler 480 for DNA quantification with universal primers of DG74 (5'-AGGAGGTGATCCAACCGCA-3') and RW01 (5'-AACTGGAGGAAGGTGGGGAT-3') targeting on a conserved 370-bp region of 16S rRNA gene. The DNA loss rate was determined by the ratio of DNA concentrations in the supernatant sample to total DNA concentrations in the entire sample (supernatant sample + pellet sample). |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_974989 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Viability, culturability and DNA loss rate obtained for collected bacteria after filter and impingement-based sampling Lee, Shu-An Low, Yee-Lynn Adhikari, Atin Wang, Wei-Kuang Liang, Chih-Ming Chang, Li-Te Tsai, Chuen-Jinn culturability; Culturability; DNA loss; DNA loss rate; Escherichia coli; EXP; Experiment; FengChiaUni_bacteria_exp; filter; Laboratory experiment; liquid impingement; Sample method; Sampling time; Type of study; viability; Viability Viability, culturability and DNA loss rate were obtained for collected bacteria after filter and impingement-based sampling. The sample extracts were spread on TSA plates. The inoculated TSA plates were incubated at 37℃ for 1 day, and colony forming units (CFUs) were counted for culturable fungi. Dual-fluorescence method for accurate viability analysis of test samples was performed. With an appropriate mixture of AO/PI, total cells were stained with AO whereas damaged cells were stained with PI. An epifluorescence microscope was used to count the test sample's bacterial cells on the filter at a magnification of 400x. The culturability is determined by percentage of CCFU in CAO while percentage of (CAO-CPI) in CAO is for viability. The qPCR was performed on LightCycler 480 for DNA quantification with universal primers of DG74 (5'-AGGAGGTGATCCAACCGCA-3') and RW01 (5'-AACTGGAGGAAGGTGGGGAT-3') targeting on a conserved 370-bp region of 16S rRNA gene. The DNA loss rate was determined by the ratio of DNA concentrations in the supernatant sample to total DNA concentrations in the entire sample (supernatant sample + pellet sample). |
| title | Viability, culturability and DNA loss rate obtained for collected bacteria after filter and impingement-based sampling |
| topic | culturability; Culturability; DNA loss; DNA loss rate; Escherichia coli; EXP; Experiment; FengChiaUni_bacteria_exp; filter; Laboratory experiment; liquid impingement; Sample method; Sampling time; Type of study; viability; Viability |
| url | https://doi.org/10.1594/PANGAEA.974989 |