Incubation experiments characterizing nitrate reduction in the water column of the Lake Lugano North Basin, Switzerland
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| Natura: | Dataset Open Access |
| Lingua: | en |
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PANGAEA
2025
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| _version_ | 1867171049240526848 |
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| author | Tischer, Jana Zopfi, Jakob Lehmann, Moritz F |
| author_facet | Tischer, Jana Zopfi, Jakob Lehmann, Moritz F |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | We performed incubation experiments with added nitrate (and additional sulfide or acetate) using water samples from the Lake Lugano North Basin, to determine the N and O isotope effects of nitrate reduction in natural lake water consortia. Samples were collected during field campaigns in November 2016, February 2017, October 2017, and April 2018 at the deepest point of the lake, using Niskin bottles at depths between 95 m and 155 m. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_977626 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Incubation experiments characterizing nitrate reduction in the water column of the Lake Lugano North Basin, Switzerland Tischer, Jana Zopfi, Jakob Lehmann, Moritz F 15N-tracer; 16S rRNA gene sequencing; Ammonium; Ammonium production rate; Ammonium production rate, standard error; Analysis; anammox; anoxic hypolimnion; Calculated; Chemiluminescence detection of NO, Vanadium(III) method (Braman and Hendrix 1989); DATE/TIME; Dechloromonas, relative 16S rRNA clone frequency; Denitrification; DEPTH, water; Desulfomonile, relative 16S rRNA clone frequency; dissimilatory nitrate reduction to ammonium; Elemental sulfur production rate; Elemental sulfur production rate, standard error; Experiment; GC-IRMS, denitrifier method (Sigman et al. 2001); HPLC, Fluorescence detection, monobromobimane method (Fahey and Newton 1987); Hydrogenophaga, relative 16S rRNA clone frequency; Hydrogen sulfide; Hydrogen sulfide, production rate; Hydrogen sulfide, production rate, standard error; Ion chromatography (940 Professional IC Vario, Metrohm); Lake; LakeLugano; meromixis; MULT; Multiple investigations; nitrate; Nitrate; Nitrate isotopes; Nitrate production rate; Nitrate production rate, standard error; nitrate reduction; nitrification; Nitrite; Nitrogen; nitrogen cycle; Nitrogen isotope effect; Nitrogen isotope effect, standard error; nitrogen isotopes; Nitrospira, relative 16S rRNA clone frequency; Number of samples; oxycline; Oxygen isotope effect; Oxygen isotope effect, standard error; Photometer, methylene blue (Cline, 1969); Photometrical detection, Griess reagent method (Hansen and Koroleff 1999); Photometrical detection, Phenol method (Hansen and Koroleff 1999); Pseudomonas, relative 16S rRNA clone frequency; Replicate; Reversed phase high performance liquid chromatography (RP-HPLC) (Zopfi et al. 2008); Rhodoferax, relative 16S rRNA clone frequency; Sulfate; Sulfate production rate; Sulfate production rate, standard error; Sulfite; Sulfite production rate; Sulfite production rate, standard error; Sulfur; Sulfur, elemental; sulfur cycle; Sulfuricurvum, relative 16S rRNA clone frequency; Switzerland; Thiobacillus, relative 16S rRNA clone frequency; Thiosulfate; Thiosulfate production rate; Thiosulfate production rate, standard error; Time, incubation; Treatment: electron donor; Treatment: electron donor concentration; water column; Δδ18O/Δδ15N ratio; Δδ18O/Δδ15N ratio, standard error; δ15N, nitrate; δ18O, nitrate We performed incubation experiments with added nitrate (and additional sulfide or acetate) using water samples from the Lake Lugano North Basin, to determine the N and O isotope effects of nitrate reduction in natural lake water consortia. Samples were collected during field campaigns in November 2016, February 2017, October 2017, and April 2018 at the deepest point of the lake, using Niskin bottles at depths between 95 m and 155 m. |
| title | Incubation experiments characterizing nitrate reduction in the water column of the Lake Lugano North Basin, Switzerland |
| topic | 15N-tracer; 16S rRNA gene sequencing; Ammonium; Ammonium production rate; Ammonium production rate, standard error; Analysis; anammox; anoxic hypolimnion; Calculated; Chemiluminescence detection of NO, Vanadium(III) method (Braman and Hendrix 1989); DATE/TIME; Dechloromonas, relative 16S rRNA clone frequency; Denitrification; DEPTH, water; Desulfomonile, relative 16S rRNA clone frequency; dissimilatory nitrate reduction to ammonium; Elemental sulfur production rate; Elemental sulfur production rate, standard error; Experiment; GC-IRMS, denitrifier method (Sigman et al. 2001); HPLC, Fluorescence detection, monobromobimane method (Fahey and Newton 1987); Hydrogenophaga, relative 16S rRNA clone frequency; Hydrogen sulfide; Hydrogen sulfide, production rate; Hydrogen sulfide, production rate, standard error; Ion chromatography (940 Professional IC Vario, Metrohm); Lake; LakeLugano; meromixis; MULT; Multiple investigations; nitrate; Nitrate; Nitrate isotopes; Nitrate production rate; Nitrate production rate, standard error; nitrate reduction; nitrification; Nitrite; Nitrogen; nitrogen cycle; Nitrogen isotope effect; Nitrogen isotope effect, standard error; nitrogen isotopes; Nitrospira, relative 16S rRNA clone frequency; Number of samples; oxycline; Oxygen isotope effect; Oxygen isotope effect, standard error; Photometer, methylene blue (Cline, 1969); Photometrical detection, Griess reagent method (Hansen and Koroleff 1999); Photometrical detection, Phenol method (Hansen and Koroleff 1999); Pseudomonas, relative 16S rRNA clone frequency; Replicate; Reversed phase high performance liquid chromatography (RP-HPLC) (Zopfi et al. 2008); Rhodoferax, relative 16S rRNA clone frequency; Sulfate; Sulfate production rate; Sulfate production rate, standard error; Sulfite; Sulfite production rate; Sulfite production rate, standard error; Sulfur; Sulfur, elemental; sulfur cycle; Sulfuricurvum, relative 16S rRNA clone frequency; Switzerland; Thiobacillus, relative 16S rRNA clone frequency; Thiosulfate; Thiosulfate production rate; Thiosulfate production rate, standard error; Time, incubation; Treatment: electron donor; Treatment: electron donor concentration; water column; Δδ18O/Δδ15N ratio; Δδ18O/Δδ15N ratio, standard error; δ15N, nitrate; δ18O, nitrate |
| url | https://doi.org/10.1594/PANGAEA.977626 |