Incubation experiments characterizing nitrate reduction in the water column of Lake La Cruz, Spain

Fuente: PANGAEA
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Auteurs principaux: Tischer, Jana, Zopfi, Jakob, Frey, Claudia, Lehmann, Moritz F
Format: Dataset Open Access
Langue:en
Publié: PANGAEA 2025
Sujets:
15N-tracer; 16S rRNA gene sequencing; Ammonium; Ammonium production rate; Ammonium production rate, standard error; Analysis; anammox; anoxic hypolimnion; Calculated; Chemiluminescence detection of NO, Vanadium(III) method (Braman and Hendrix 1989); DATE/TIME; Dechloromonas, relative 16S rRNA clone frequency; Denitrification; DEPTH, water; Desulfomonile, relative 16S rRNA clone frequency; dissimilatory nitrate reduction to ammonium; GC-IRMS, denitrifier method (Sigman et al. 2001); Hydrogenophaga, relative 16S rRNA clone frequency; Lake; Lake_LaCruz; meromixis; MULT; Multiple investigations; nitrate; Nitrate; Nitrate isotopes; Nitrate production rate; Nitrate production rate, standard error; nitrate reduction; nitrification; Nitrite; Nitrogen; nitrogen cycle; Nitrogen isotope effect; Nitrogen isotope effect, standard error; nitrogen isotopes; Nitrospira, relative 16S rRNA clone frequency; Number of samples; oxycline; Oxygen isotope effect; Oxygen isotope effect, standard error; Photometrical detection, Griess reagent method (Hansen and Koroleff 1999); Photometrical detection, Phenol method (Hansen and Koroleff 1999); Pseudomonas, relative 16S rRNA clone frequency; Replicate; Rhodoferax, relative 16S rRNA clone frequency; Spain; Sulfur; sulfur cycle; Sulfuricurvum, relative 16S rRNA clone frequency; Thiobacillus, relative 16S rRNA clone frequency; Time, incubation; Treatment: electron donor; Treatment: electron donor concentration; water column; Δδ18O/Δδ15N ratio; Δδ18O/Δδ15N ratio, standard error; δ15N, nitrate; δ18O, nitrate
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author Tischer, Jana
Zopfi, Jakob
Frey, Claudia
Lehmann, Moritz F
author_facet Tischer, Jana
Zopfi, Jakob
Frey, Claudia
Lehmann, Moritz F
collection Datos científicos de ciencias marinas y ambientales
contents We performed incubation experiments with added nitrate (and additional sulfide) using water samples from the meromictic Lake La Cruz, Spain, to determine the N and O isotope effects of nitrate reduction in natural lake water consortia. Samples were collected during field campaigns in March 2015 and March 2017 at the deepest point of the lake, using an in situ pump at depths of 14.5 m or 17 m.
format Dataset Open Access
id pangaea_https___doi_org_10_1594_PANGAEA_977627
institution PANGAEA
language en
publishDate 2025
publisher PANGAEA
record_format pangaea
spellingShingle Incubation experiments characterizing nitrate reduction in the water column of Lake La Cruz, Spain
Tischer, Jana
Zopfi, Jakob
Frey, Claudia
Lehmann, Moritz F
15N-tracer; 16S rRNA gene sequencing; Ammonium; Ammonium production rate; Ammonium production rate, standard error; Analysis; anammox; anoxic hypolimnion; Calculated; Chemiluminescence detection of NO, Vanadium(III) method (Braman and Hendrix 1989); DATE/TIME; Dechloromonas, relative 16S rRNA clone frequency; Denitrification; DEPTH, water; Desulfomonile, relative 16S rRNA clone frequency; dissimilatory nitrate reduction to ammonium; GC-IRMS, denitrifier method (Sigman et al. 2001); Hydrogenophaga, relative 16S rRNA clone frequency; Lake; Lake_LaCruz; meromixis; MULT; Multiple investigations; nitrate; Nitrate; Nitrate isotopes; Nitrate production rate; Nitrate production rate, standard error; nitrate reduction; nitrification; Nitrite; Nitrogen; nitrogen cycle; Nitrogen isotope effect; Nitrogen isotope effect, standard error; nitrogen isotopes; Nitrospira, relative 16S rRNA clone frequency; Number of samples; oxycline; Oxygen isotope effect; Oxygen isotope effect, standard error; Photometrical detection, Griess reagent method (Hansen and Koroleff 1999); Photometrical detection, Phenol method (Hansen and Koroleff 1999); Pseudomonas, relative 16S rRNA clone frequency; Replicate; Rhodoferax, relative 16S rRNA clone frequency; Spain; Sulfur; sulfur cycle; Sulfuricurvum, relative 16S rRNA clone frequency; Thiobacillus, relative 16S rRNA clone frequency; Time, incubation; Treatment: electron donor; Treatment: electron donor concentration; water column; Δδ18O/Δδ15N ratio; Δδ18O/Δδ15N ratio, standard error; δ15N, nitrate; δ18O, nitrate
We performed incubation experiments with added nitrate (and additional sulfide) using water samples from the meromictic Lake La Cruz, Spain, to determine the N and O isotope effects of nitrate reduction in natural lake water consortia. Samples were collected during field campaigns in March 2015 and March 2017 at the deepest point of the lake, using an in situ pump at depths of 14.5 m or 17 m.
title Incubation experiments characterizing nitrate reduction in the water column of Lake La Cruz, Spain
topic 15N-tracer; 16S rRNA gene sequencing; Ammonium; Ammonium production rate; Ammonium production rate, standard error; Analysis; anammox; anoxic hypolimnion; Calculated; Chemiluminescence detection of NO, Vanadium(III) method (Braman and Hendrix 1989); DATE/TIME; Dechloromonas, relative 16S rRNA clone frequency; Denitrification; DEPTH, water; Desulfomonile, relative 16S rRNA clone frequency; dissimilatory nitrate reduction to ammonium; GC-IRMS, denitrifier method (Sigman et al. 2001); Hydrogenophaga, relative 16S rRNA clone frequency; Lake; Lake_LaCruz; meromixis; MULT; Multiple investigations; nitrate; Nitrate; Nitrate isotopes; Nitrate production rate; Nitrate production rate, standard error; nitrate reduction; nitrification; Nitrite; Nitrogen; nitrogen cycle; Nitrogen isotope effect; Nitrogen isotope effect, standard error; nitrogen isotopes; Nitrospira, relative 16S rRNA clone frequency; Number of samples; oxycline; Oxygen isotope effect; Oxygen isotope effect, standard error; Photometrical detection, Griess reagent method (Hansen and Koroleff 1999); Photometrical detection, Phenol method (Hansen and Koroleff 1999); Pseudomonas, relative 16S rRNA clone frequency; Replicate; Rhodoferax, relative 16S rRNA clone frequency; Spain; Sulfur; sulfur cycle; Sulfuricurvum, relative 16S rRNA clone frequency; Thiobacillus, relative 16S rRNA clone frequency; Time, incubation; Treatment: electron donor; Treatment: electron donor concentration; water column; Δδ18O/Δδ15N ratio; Δδ18O/Δδ15N ratio, standard error; δ15N, nitrate; δ18O, nitrate
url https://doi.org/10.1594/PANGAEA.977627