Physiologial charaterization of the Arctic diatom Thalassisira hyalina under different acclimation temperatures
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2025
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| _version_ | 1867168236068405248 |
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| author | Rehder, Linda |
| author_facet | Rehder, Linda |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | This experiment aimed to understand a the high plasticity of Arctic diatoms towards ocean warming, with a special regard to regulatory mechanisms of photosynthetic and respiratory processes. We therefore assessed the physiological performance of the Arctic diatom Thalassiosira hyalina under sub-optimal (2°C), optimal (6°C) and supra-optimal (10°C) temperatures. The acclimation irradiance was set to 30 µmol photons m-2 s-1 under continuous illumination (24h) and nutrients were repleted. the experiment took place in Winter 2023/2024 in laboratories of the Alfred-Wegener-Institute Bremerhaven. We assessed growth rates, quotas of particulate organic carbon and chlorophyll a, photophyisology using fast repetition rate fluorometry (FRRF) following Schuback et al. 2021, and gas fluxes of O2 and CO2 corresponding to photosynthetic and respiratory processes using membrane-inlet mass-spectrometry (MIMS) following Fock and Sültemeyer 1989 and Rehder et al. 2023. MIMS measurements were done in pH buffered assay medium (pH 7.8) including carbonic anhydrase and equilibrated to ~20% 18O2, to distinguish between photosynthetic O2 production and respiratory O2 consumption in the light. Furthermore, both FRRF and MIMS measurements were performed in photosynthesis-irradiance assays to obtained maximal rates of electron transport, gross O2 production and gross C-fixation. The dataset indicates a metabolic coupling of chloroplasts and mitochondria, which likely reroutes excess reductant from the chloroplasts into mitochondrial respiration. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_979803 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Physiologial charaterization of the Arctic diatom Thalassisira hyalina under different acclimation temperatures Rehder, Linda Arctic; Calculated; Calculation according to Rehder et al. (2023); Carbon, organic, particulate, per cell; Carbon, organic, particulate, production per cell; C fixation; Chlorophyll a/particulate organic carbon ratio; Chlorophyll a per cell; Daily cell counting, Beckmann coulter Counter; Diatom; Electron transport rate, absolute; Electron transport rate, relative; Elemental analyzer, EuroVector, EA 3000; Experiment; Fast repetition rate fluorometry, calculated from PI cuve fitting; Fast repetition rate fluorometry, calculated from PI cuve fitting after Trimborn et al. 2017; Fast repetition rate fluorometry, calculated from PI cuve fitting from absolute electron transport rates; Fast repetition rate fluorometry, calculated from PI cuve fitting from absolute electron transport rates after Schuback et al. 2021; Fast repetition rate fluorometry, dark acclimated; Functional absorption cross sections of photosystem II reaction centers; Generation; Gross photosynthetic carbon uptake rate, per chlorophyll a; Gross photosynthetic oxygen release rate, per chlorophyll a; Growth rate; Laboratory; Laboratory experiment; Laboratory fluorometer, Turner Designs, Trilogy; Light acclimation index; Light enhanced dark respiration, oxygen, per chlorophyll a; Light use efficiency; Maximal absolute electron transport rate; Maximum quantum yield of photosystem II; Medium; Membrane-inlet mass-spectrometry, after Sültemeyer & Fock 1986; metabolic coupling; Net photosynthetic carbon uptake rate, per chlorophyll a; Net photosynthetic oxygen release rate, per chlorophyll a; Optical absorption cross section of photosystem II reaction centers; Photosynthesis; Photosynthetic quotient; physiological plasticity; Quantum yield of non photochemical non regulated energy dissipation of photosystem II; Respiration; Respiratory quotient; Respiratory release rate, carbon dioxide, dark, per chlorophyll a; Respiratory uptake rate, oxygen, dark, per chlorophyll a; Respiratory uptake rate, oxygen, in light, per chlorophyll a; Sample ID; Sampling date/time, experiment; Species, unique identification; Strain; Temperate; Time constant for the initial part of an single turnover relaxation phase; Treatment: light:dark cycle; Treatment: light intensity; Treatment: partial pressure of carbon dioxide; Treatment: salinity; Treatment: temperature; Type of study This experiment aimed to understand a the high plasticity of Arctic diatoms towards ocean warming, with a special regard to regulatory mechanisms of photosynthetic and respiratory processes. We therefore assessed the physiological performance of the Arctic diatom Thalassiosira hyalina under sub-optimal (2°C), optimal (6°C) and supra-optimal (10°C) temperatures. The acclimation irradiance was set to 30 µmol photons m-2 s-1 under continuous illumination (24h) and nutrients were repleted. the experiment took place in Winter 2023/2024 in laboratories of the Alfred-Wegener-Institute Bremerhaven. We assessed growth rates, quotas of particulate organic carbon and chlorophyll a, photophyisology using fast repetition rate fluorometry (FRRF) following Schuback et al. 2021, and gas fluxes of O2 and CO2 corresponding to photosynthetic and respiratory processes using membrane-inlet mass-spectrometry (MIMS) following Fock and Sültemeyer 1989 and Rehder et al. 2023. MIMS measurements were done in pH buffered assay medium (pH 7.8) including carbonic anhydrase and equilibrated to ~20% 18O2, to distinguish between photosynthetic O2 production and respiratory O2 consumption in the light. Furthermore, both FRRF and MIMS measurements were performed in photosynthesis-irradiance assays to obtained maximal rates of electron transport, gross O2 production and gross C-fixation. The dataset indicates a metabolic coupling of chloroplasts and mitochondria, which likely reroutes excess reductant from the chloroplasts into mitochondrial respiration. |
| title | Physiologial charaterization of the Arctic diatom Thalassisira hyalina under different acclimation temperatures |
| topic | Arctic; Calculated; Calculation according to Rehder et al. (2023); Carbon, organic, particulate, per cell; Carbon, organic, particulate, production per cell; C fixation; Chlorophyll a/particulate organic carbon ratio; Chlorophyll a per cell; Daily cell counting, Beckmann coulter Counter; Diatom; Electron transport rate, absolute; Electron transport rate, relative; Elemental analyzer, EuroVector, EA 3000; Experiment; Fast repetition rate fluorometry, calculated from PI cuve fitting; Fast repetition rate fluorometry, calculated from PI cuve fitting after Trimborn et al. 2017; Fast repetition rate fluorometry, calculated from PI cuve fitting from absolute electron transport rates; Fast repetition rate fluorometry, calculated from PI cuve fitting from absolute electron transport rates after Schuback et al. 2021; Fast repetition rate fluorometry, dark acclimated; Functional absorption cross sections of photosystem II reaction centers; Generation; Gross photosynthetic carbon uptake rate, per chlorophyll a; Gross photosynthetic oxygen release rate, per chlorophyll a; Growth rate; Laboratory; Laboratory experiment; Laboratory fluorometer, Turner Designs, Trilogy; Light acclimation index; Light enhanced dark respiration, oxygen, per chlorophyll a; Light use efficiency; Maximal absolute electron transport rate; Maximum quantum yield of photosystem II; Medium; Membrane-inlet mass-spectrometry, after Sültemeyer & Fock 1986; metabolic coupling; Net photosynthetic carbon uptake rate, per chlorophyll a; Net photosynthetic oxygen release rate, per chlorophyll a; Optical absorption cross section of photosystem II reaction centers; Photosynthesis; Photosynthetic quotient; physiological plasticity; Quantum yield of non photochemical non regulated energy dissipation of photosystem II; Respiration; Respiratory quotient; Respiratory release rate, carbon dioxide, dark, per chlorophyll a; Respiratory uptake rate, oxygen, dark, per chlorophyll a; Respiratory uptake rate, oxygen, in light, per chlorophyll a; Sample ID; Sampling date/time, experiment; Species, unique identification; Strain; Temperate; Time constant for the initial part of an single turnover relaxation phase; Treatment: light:dark cycle; Treatment: light intensity; Treatment: partial pressure of carbon dioxide; Treatment: salinity; Treatment: temperature; Type of study |
| url | https://doi.org/10.1594/PANGAEA.979803 |