Total carbohydrates quantified in sediment cores from coastal vegetated ecosystems
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2025
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| author | Hellige, Inga Akeerath Mundanatt, Aman Fett, Theresa-Marie Wölfelschneider, Mirco Prugger, Jennifer Geuer, Jana K Reents, Svenja |
| author_facet | Hellige, Inga Akeerath Mundanatt, Aman Fett, Theresa-Marie Wölfelschneider, Mirco Prugger, Jennifer Geuer, Jana K Reents, Svenja |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | 50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. The total carbohydrate content was assessed using the phenol-sulfuric acid assay (Dubois et al., 1956). Briefly, 100 µL of resuspended samples or extracts were mixed with 100 µL of 5% phenol solution, followed by the addition of 500 µL of concentrated sulfuric acid. The reaction mixture was incubated at room temperature for 10 minutes, then further incubated at 30°C for 20 minutes. Absorbance at 490 nm was measured using a Spectramax Id3 plate reader (Molecular Devices) and quantified against a glucose standard curve. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_979910 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Total carbohydrates quantified in sediment cores from coastal vegetated ecosystems Hellige, Inga Akeerath Mundanatt, Aman Fett, Theresa-Marie Wölfelschneider, Mirco Prugger, Jennifer Geuer, Jana K Reents, Svenja Carbohydrates, total, per unit sediment dry mass; carbon dioxide removal; CDRmare; coastal vegetated ecosystems; Colombia; Comment; Country; DAM CDRmare - sea4soCiety: Searching for solutions for Carbon-sequestration in coastal ecosystems; DATE/TIME; DEPTH, sediment/rock; Depth, sediment/rock, bottom/maximum; Depth, sediment/rock, top/minimum; Ecosystem; Event label; GER_HAH_SG_P4_SC; GER_HAH_SG_P6_SC; GER_HAH_SG_P8_SC; GER_HAH_SM_P2_SC; GER_HAH_SM_P5_SC; GER_HAH_SM_P7_SC; GER_HAH_UV_P4_SC; GER_HAH_UV_P5_SC; GER_HAH_UV_P6_SC; GER_HEI_SG_P3_SC; GER_HEI_SG_P6_SC; GER_HEI_SG_P8_SC; GER_HEI_SM_P1_SC; GER_HEI_SM_P3_SC; GER_HEI_SM_P4_SC; GER_HEI_SM_P9_SC; GER_HEI_UV_P3_SC; GER_HEI_UV_P5_SC; GER_HEI_UV_P7_SC; GER_MET_SG_P4_SC; GER_MET_SG_P5_SC; GER_MET_SG_P6_SC; GER_MET_SM_P1_SC; GER_MET_SM_P4_SC; GER_MET_SM_P7_SC; GER_MET_UV_P4_SC; GER_MET_UV_P5_SC; GER_MET_UV_P6_SC; GER_NES_SG_P4_SC; GER_NES_SG_P5_SC; GER_NES_SG_P6_SC; GER_NES_SM_P3_SC; GER_NES_SM_P6_SC; GER_NES_SM_P8_SC; GER_NES_SM_P9_SC; GER_NES_UV_P4_SC; GER_NES_UV_P5_SC; GER_NES_UV_P6_SC; GER_SCH_SM_P4_SC; GER_SCH_SM_P5_SC; GER_SCH_SM_P6_SC; GER_SCH_SM_P7_SC; GER_WEN_SG_P5_SC; GER_WEN_SG_P7_SC; GER_WEN_SG_P9_SC; GER_WEN_SM_P5_SC; GER_WEN_SM_P7_SC; GER_WEN_SM_P9_SC; GER_WEN_UV_P3_SC; GER_WEN_UV_P5_SC; GER_WEN_UV_P7_SC; Glycans; Identification; LATITUDE; Layer description; Layer number; Location; LONGITUDE; marine carbon sequestration; MG1_1_SC; MG1_2_SC; MG2_1_SC; MG2_2_SC; MG2_3_SC; MG3_1_SC; MG3_2_SC; MYS_LAN_MG_P1_SC; MYS_LAN_MG_P5_SC; MYS_LAN_MG_P9_SC; MYS_LAN_SG_P1_SC; MYS_LAN_SG_P3_SC; MYS_LAN_SG_P5_SC; MYS_LAN_UV_P1_SC; MYS_LAN_UV_P3_SC; MYS_LAN_UV_P5_SC; MYS_PAK_MG_P5_SC; MYS_PAK_MG_P9_SC; MYS_PAK_SM_P1_SC; MYS_SET_MG_P2_SC; MYS_SET_MG_P5_SC; MYS_SET_MG_P8_SC; MYS_SET_SG_P1_SC; MYS_SET_SG_P2_SC; MYS_SET_SG_P4_SC; MYS_SET_SM_P1_SC; MYS_SET_SM_P3_SC; MYS_SET_SM_P5_SC; MYS_SET_UV_P1_SC; MYS_SET_UV_P2_SC; MYS_SET_UV_P3_SC; Ocean and sea region; PEATC; Peat corer; Phenol-sulfuric acid assay according to Dubois et al. (1956); Research Mission of the German Marine Research Alliance (DAM): Marine carbon sinks in decarbonisation pathways; s4s_COL-Land_2022; s4s_GER-Land_2021_NorthSea; s4s_GER-Land_2022_BalticSea; s4s_GER-Land_2022_NorthSea; s4s_MYS-Land_2022; s4s_MYS-Land_2023; Sample code/label; Sampling on land; sea4soCiety; SG1_1_SC; SG1_2_SC; SG1_3_SC; SG2_1_SC; SG2_2_SC; SG2_3_SC; SG3_1_SC; SG3_2_SC; SG3_3_SC; UV1_1_SC; UV1_2_SC; UV1_3_SC; UV2_2_SC; UV2_3_SC; UV3_1_SC; UV3_2_SC; UV3_3_SC 50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. The total carbohydrate content was assessed using the phenol-sulfuric acid assay (Dubois et al., 1956). Briefly, 100 µL of resuspended samples or extracts were mixed with 100 µL of 5% phenol solution, followed by the addition of 500 µL of concentrated sulfuric acid. The reaction mixture was incubated at room temperature for 10 minutes, then further incubated at 30°C for 20 minutes. Absorbance at 490 nm was measured using a Spectramax Id3 plate reader (Molecular Devices) and quantified against a glucose standard curve. |
| title | Total carbohydrates quantified in sediment cores from coastal vegetated ecosystems |
| topic | Carbohydrates, total, per unit sediment dry mass; carbon dioxide removal; CDRmare; coastal vegetated ecosystems; Colombia; Comment; Country; DAM CDRmare - sea4soCiety: Searching for solutions for Carbon-sequestration in coastal ecosystems; DATE/TIME; DEPTH, sediment/rock; Depth, sediment/rock, bottom/maximum; Depth, sediment/rock, top/minimum; Ecosystem; Event label; GER_HAH_SG_P4_SC; GER_HAH_SG_P6_SC; GER_HAH_SG_P8_SC; GER_HAH_SM_P2_SC; GER_HAH_SM_P5_SC; GER_HAH_SM_P7_SC; GER_HAH_UV_P4_SC; GER_HAH_UV_P5_SC; GER_HAH_UV_P6_SC; GER_HEI_SG_P3_SC; GER_HEI_SG_P6_SC; GER_HEI_SG_P8_SC; GER_HEI_SM_P1_SC; GER_HEI_SM_P3_SC; GER_HEI_SM_P4_SC; GER_HEI_SM_P9_SC; GER_HEI_UV_P3_SC; GER_HEI_UV_P5_SC; GER_HEI_UV_P7_SC; GER_MET_SG_P4_SC; GER_MET_SG_P5_SC; GER_MET_SG_P6_SC; GER_MET_SM_P1_SC; GER_MET_SM_P4_SC; GER_MET_SM_P7_SC; GER_MET_UV_P4_SC; GER_MET_UV_P5_SC; GER_MET_UV_P6_SC; GER_NES_SG_P4_SC; GER_NES_SG_P5_SC; GER_NES_SG_P6_SC; GER_NES_SM_P3_SC; GER_NES_SM_P6_SC; GER_NES_SM_P8_SC; GER_NES_SM_P9_SC; GER_NES_UV_P4_SC; GER_NES_UV_P5_SC; GER_NES_UV_P6_SC; GER_SCH_SM_P4_SC; GER_SCH_SM_P5_SC; GER_SCH_SM_P6_SC; GER_SCH_SM_P7_SC; GER_WEN_SG_P5_SC; GER_WEN_SG_P7_SC; GER_WEN_SG_P9_SC; GER_WEN_SM_P5_SC; GER_WEN_SM_P7_SC; GER_WEN_SM_P9_SC; GER_WEN_UV_P3_SC; GER_WEN_UV_P5_SC; GER_WEN_UV_P7_SC; Glycans; Identification; LATITUDE; Layer description; Layer number; Location; LONGITUDE; marine carbon sequestration; MG1_1_SC; MG1_2_SC; MG2_1_SC; MG2_2_SC; MG2_3_SC; MG3_1_SC; MG3_2_SC; MYS_LAN_MG_P1_SC; MYS_LAN_MG_P5_SC; MYS_LAN_MG_P9_SC; MYS_LAN_SG_P1_SC; MYS_LAN_SG_P3_SC; MYS_LAN_SG_P5_SC; MYS_LAN_UV_P1_SC; MYS_LAN_UV_P3_SC; MYS_LAN_UV_P5_SC; MYS_PAK_MG_P5_SC; MYS_PAK_MG_P9_SC; MYS_PAK_SM_P1_SC; MYS_SET_MG_P2_SC; MYS_SET_MG_P5_SC; MYS_SET_MG_P8_SC; MYS_SET_SG_P1_SC; MYS_SET_SG_P2_SC; MYS_SET_SG_P4_SC; MYS_SET_SM_P1_SC; MYS_SET_SM_P3_SC; MYS_SET_SM_P5_SC; MYS_SET_UV_P1_SC; MYS_SET_UV_P2_SC; MYS_SET_UV_P3_SC; Ocean and sea region; PEATC; Peat corer; Phenol-sulfuric acid assay according to Dubois et al. (1956); Research Mission of the German Marine Research Alliance (DAM): Marine carbon sinks in decarbonisation pathways; s4s_COL-Land_2022; s4s_GER-Land_2021_NorthSea; s4s_GER-Land_2022_BalticSea; s4s_GER-Land_2022_NorthSea; s4s_MYS-Land_2022; s4s_MYS-Land_2023; Sample code/label; Sampling on land; sea4soCiety; SG1_1_SC; SG1_2_SC; SG1_3_SC; SG2_1_SC; SG2_2_SC; SG2_3_SC; SG3_1_SC; SG3_2_SC; SG3_3_SC; UV1_1_SC; UV1_2_SC; UV1_3_SC; UV2_2_SC; UV2_3_SC; UV3_1_SC; UV3_2_SC; UV3_3_SC |
| url | https://doi.org/10.1594/PANGAEA.979910 |