Polysaccharides quantified in sediment cores from coastal vegetated ecosystems
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| Natura: | Dataset Open Access |
| Lingua: | en |
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PANGAEA
2025
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| author | Hellige, Inga Akeerath Mundanatt, Aman Fett, Theresa-Marie Wölfelschneider, Mirco Prugger, Jennifer Geuer, Jana K Reents, Svenja |
| author_facet | Hellige, Inga Akeerath Mundanatt, Aman Fett, Theresa-Marie Wölfelschneider, Mirco Prugger, Jennifer Geuer, Jana K Reents, Svenja |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | 50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. Polysaccharides were screened using microarray analysis following the method described by Vidal-Melgosa et al. (2022). Briefly, sediment extracts from MilliQ-water and EDTA were combined in equal volumes, and 30 µL of the mixture was transferred into wells of 384-microwell plates. Two consecutive two-fold dilutions were performed using a printing buffer (55.2% glycerol, 44% water, 0.8% Triton X-100). The plates were then centrifuged at 3,500 × g for 10 minutes at 15 °C. Each microarray was individually probed with a monoclonal antibody (mAb), and binding was detected using a secondary antibody conjugated to alkaline phosphatase. In the presence of its substrate, this reaction produced a colorimetric signal. Developed arrays were scanned at 2400 dots per inch, and binding signal intensity was quantified using Array-Pro Analyzer 6.3 software (Media Cybernetics). |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_979911 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Polysaccharides quantified in sediment cores from coastal vegetated ecosystems Hellige, Inga Akeerath Mundanatt, Aman Fett, Theresa-Marie Wölfelschneider, Mirco Prugger, Jennifer Geuer, Jana K Reents, Svenja Alginate mannuronate-guluronate, relative BAM7 antibody signal; alpha-1,5-L-arabinan, relative LM6 antibody signal; alpha-1,5-L-arabinan, relative LM6-M antibody signal; Arabinogalactan protein glycan, relative JIM13 antibody signal; Arabinogalactan protein glycan, relative JIM8 antibody signal; Arabinogalactan protein glycan, relative MAC207 antibody signal; beta-1,3-D-glucan, relative BS-400-2 antibody signal; beta-1,4-D-galactan, relative LM5 antibody signal; carbon dioxide removal; CDRmare; coastal vegetated ecosystems; Colombia; Country; DAM CDRmare - sea4soCiety: Searching for solutions for Carbon-sequestration in coastal ecosystems; DATE/TIME; DEPTH, sediment/rock; Depth, sediment/rock, bottom/maximum; Depth, sediment/rock, top/minimum; Depth comment; Ecosystem; Event label; Fucan, sulfated epitope, relative BAM2 antibody signal; Fucan, sulfated epitope, relative BAM4 antibody signal; GER_HAH_SM_P7_SC; GER_HEI_SM_P1_SC; GER_HEI_SM_P3_SC; GER_HEI_SM_P4_SC; GER_MET_SM_P1_SC; GER_NES_SM_P6_SC; GER_NES_SM_P8_SC; GER_NES_SM_P9_SC; GER_SCH_SM_P4_SC; GER_SCH_SM_P5_SC; GER_SCH_SM_P6_SC; GER_SCH_SM_P7_SC; GER_WEN_SM_P5_SC; Glycans; Grass xylan, relative LM27 antibody signal; Homogalacturonan, methyl-esterified, relative JIM7 antibody signal; Homogalacturonan, partially methyl-esterified/de-esterified, relative JIM5 antibody signal; LATITUDE; Location; LONGITUDE; marine carbon sequestration; MG1_1_SC; MG1_2_SC; MG1_3_SC; MG2_2_SC; MG3_1_SC; Microarray analysis according to Vidal-Melgosa et al. (2022); PEATC; Peat corer; Research Mission of the German Marine Research Alliance (DAM): Marine carbon sinks in decarbonisation pathways; Rhamnogalacturonan I backbone, relative INRA-RU1 antibody signal; s4s_COL-Land_2022; s4s_GER-Land_2021_NorthSea; s4s_GER-Land_2022_BalticSea; s4s_GER-Land_2022_NorthSea; Sample code/label; Sampling on land; sea4soCiety; SG2_2_SC; SG2_3_SC; SG3_3_SC; UV2_1_SC; UV2_2_SC; UV2_3_SC 50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. Polysaccharides were screened using microarray analysis following the method described by Vidal-Melgosa et al. (2022). Briefly, sediment extracts from MilliQ-water and EDTA were combined in equal volumes, and 30 µL of the mixture was transferred into wells of 384-microwell plates. Two consecutive two-fold dilutions were performed using a printing buffer (55.2% glycerol, 44% water, 0.8% Triton X-100). The plates were then centrifuged at 3,500 × g for 10 minutes at 15 °C. Each microarray was individually probed with a monoclonal antibody (mAb), and binding was detected using a secondary antibody conjugated to alkaline phosphatase. In the presence of its substrate, this reaction produced a colorimetric signal. Developed arrays were scanned at 2400 dots per inch, and binding signal intensity was quantified using Array-Pro Analyzer 6.3 software (Media Cybernetics). |
| title | Polysaccharides quantified in sediment cores from coastal vegetated ecosystems |
| topic | Alginate mannuronate-guluronate, relative BAM7 antibody signal; alpha-1,5-L-arabinan, relative LM6 antibody signal; alpha-1,5-L-arabinan, relative LM6-M antibody signal; Arabinogalactan protein glycan, relative JIM13 antibody signal; Arabinogalactan protein glycan, relative JIM8 antibody signal; Arabinogalactan protein glycan, relative MAC207 antibody signal; beta-1,3-D-glucan, relative BS-400-2 antibody signal; beta-1,4-D-galactan, relative LM5 antibody signal; carbon dioxide removal; CDRmare; coastal vegetated ecosystems; Colombia; Country; DAM CDRmare - sea4soCiety: Searching for solutions for Carbon-sequestration in coastal ecosystems; DATE/TIME; DEPTH, sediment/rock; Depth, sediment/rock, bottom/maximum; Depth, sediment/rock, top/minimum; Depth comment; Ecosystem; Event label; Fucan, sulfated epitope, relative BAM2 antibody signal; Fucan, sulfated epitope, relative BAM4 antibody signal; GER_HAH_SM_P7_SC; GER_HEI_SM_P1_SC; GER_HEI_SM_P3_SC; GER_HEI_SM_P4_SC; GER_MET_SM_P1_SC; GER_NES_SM_P6_SC; GER_NES_SM_P8_SC; GER_NES_SM_P9_SC; GER_SCH_SM_P4_SC; GER_SCH_SM_P5_SC; GER_SCH_SM_P6_SC; GER_SCH_SM_P7_SC; GER_WEN_SM_P5_SC; Glycans; Grass xylan, relative LM27 antibody signal; Homogalacturonan, methyl-esterified, relative JIM7 antibody signal; Homogalacturonan, partially methyl-esterified/de-esterified, relative JIM5 antibody signal; LATITUDE; Location; LONGITUDE; marine carbon sequestration; MG1_1_SC; MG1_2_SC; MG1_3_SC; MG2_2_SC; MG3_1_SC; Microarray analysis according to Vidal-Melgosa et al. (2022); PEATC; Peat corer; Research Mission of the German Marine Research Alliance (DAM): Marine carbon sinks in decarbonisation pathways; Rhamnogalacturonan I backbone, relative INRA-RU1 antibody signal; s4s_COL-Land_2022; s4s_GER-Land_2021_NorthSea; s4s_GER-Land_2022_BalticSea; s4s_GER-Land_2022_NorthSea; Sample code/label; Sampling on land; sea4soCiety; SG2_2_SC; SG2_3_SC; SG3_3_SC; UV2_1_SC; UV2_2_SC; UV2_3_SC |
| url | https://doi.org/10.1594/PANGAEA.979911 |