Physiological data of Pacific oyster Crassostrea (Magallana) gigas after exposure to intermittent hypoxia and the combination with (fluctuating) elevated temperature
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| Sprache: | en |
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PANGAEA
2025
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| author | Bruhns, Torben Timm, Stefan Feußner, Nina Engelhaupt, Sonja Labrenz, Matthias Wegner, K Mathias Sokolova, Inna M |
| author_facet | Bruhns, Torben Timm, Stefan Feußner, Nina Engelhaupt, Sonja Labrenz, Matthias Wegner, K Mathias Sokolova, Inna M |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Organisms in intertidal zones experience fluctuations in environmental stressors such as hypoxia and temperature. These stressors and their fluctuations often appear in combination. Combination of stressors can have different effects compared to single stressors. In this study, we investigate the physiological effects of intermittent hypoxia in combination with different temperature regimes on the Pacific Oyster Crassostrea (Magallana) gigas. The oysters were exposed to hypoxic cycles (12h hypoxia by emersion/12h submersion) at normal (15°C), elevated (30°C) or fluctuating (15°C submersion/30°C emersion) temperature for 10 days. After the last submersion phase, the gills and digestive gland were sampled. We measured markers for bioenergetics and redox-balance in the gills and digestive gland using colorimetric methods as well as a set of metabolites (predominantly amino acids, osmolytes, anaerobic end products and energetic metabolites) in the gills using LC-MS/MS. Oysters kept submerged for up to 10 days were used as controls. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_980734 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Physiological data of Pacific oyster Crassostrea (Magallana) gigas after exposure to intermittent hypoxia and the combination with (fluctuating) elevated temperature Bruhns, Torben Timm, Stefan Feußner, Nina Engelhaupt, Sonja Labrenz, Matthias Wegner, K Mathias Sokolova, Inna M Aconitate, per fresh mass; Adenosine monophosphate, per fresh mass; Alanine, per fresh mass; Antioxidant capacity, in Trolox Equivalents, per fresh mass; Arginine, per fresh mass; Asparagine, per fresh mass; Aspartate, per fresh mass; bioenergetics; C.Gigas_collection; Caliper; Carbohydrates, per fresh mass; Carnitine, per fresh mass; Citrate, per fresh mass; Citrulline, per fresh mass; crassotrea gigas; Cystine, per fresh mass; Date/time end, experiment; ecophysiology; Electron transport system activity of oyxgen; energy reserves; Experiment; Experimental treatment; Experiment duration; fluctuation; gamma-Aminobutyric acid, per fresh mass; Glutamate, per fresh mass; Glutamine, per fresh mass; Glutathione, per fresh mass; Glycine, per fresh mass; High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050; Histidine, per fresh mass; Hydroxyproline, per fresh mass; hypoxia; intertidal; invasive species; Isoleucine, per fresh mass; Königshafen, Sylt, Germany; Laboratory; Laboratory experiment; Lactate, per fresh mass; Length; Leucine, per fresh mass; Lipid peroxidation, per fresh mass; Lysine, per fresh mass; Magallana gigas; Malate, per fresh mass; Mass; metabolism; metabolome; metabolomics; Methionine, per fresh mass; Methionine sulfoxide, per fresh mass; Multi-user microplate reader, Molecular devices, SpectraMax iD3; Origin; Ornithine, per fresh mass; oxidaitive stress; oxidative damage; Oxygen; Oyster; Phenylalanine, per fresh mass; Proline, per fresh mass; Protein carbonyl, per protein mass; Proteins per fresh mass; Sample ID; Sampling date/time, experiment; Serine, per fresh mass; Species, unique identification; Specimen identification; Succinate, per fresh mass; Tank number; Taurine, per fresh mass; Temperature; Threonine, per fresh mass; tidal cycle; Tissue type; Treatment: air exposure; Treatment: salinity; Treatment: temperature; Tryptophan, per fresh mass; Type of study; Tyrosine, per fresh mass; Valine, per fresh mass; Waddensea; Width Organisms in intertidal zones experience fluctuations in environmental stressors such as hypoxia and temperature. These stressors and their fluctuations often appear in combination. Combination of stressors can have different effects compared to single stressors. In this study, we investigate the physiological effects of intermittent hypoxia in combination with different temperature regimes on the Pacific Oyster Crassostrea (Magallana) gigas. The oysters were exposed to hypoxic cycles (12h hypoxia by emersion/12h submersion) at normal (15°C), elevated (30°C) or fluctuating (15°C submersion/30°C emersion) temperature for 10 days. After the last submersion phase, the gills and digestive gland were sampled. We measured markers for bioenergetics and redox-balance in the gills and digestive gland using colorimetric methods as well as a set of metabolites (predominantly amino acids, osmolytes, anaerobic end products and energetic metabolites) in the gills using LC-MS/MS. Oysters kept submerged for up to 10 days were used as controls. |
| title | Physiological data of Pacific oyster Crassostrea (Magallana) gigas after exposure to intermittent hypoxia and the combination with (fluctuating) elevated temperature |
| topic | Aconitate, per fresh mass; Adenosine monophosphate, per fresh mass; Alanine, per fresh mass; Antioxidant capacity, in Trolox Equivalents, per fresh mass; Arginine, per fresh mass; Asparagine, per fresh mass; Aspartate, per fresh mass; bioenergetics; C.Gigas_collection; Caliper; Carbohydrates, per fresh mass; Carnitine, per fresh mass; Citrate, per fresh mass; Citrulline, per fresh mass; crassotrea gigas; Cystine, per fresh mass; Date/time end, experiment; ecophysiology; Electron transport system activity of oyxgen; energy reserves; Experiment; Experimental treatment; Experiment duration; fluctuation; gamma-Aminobutyric acid, per fresh mass; Glutamate, per fresh mass; Glutamine, per fresh mass; Glutathione, per fresh mass; Glycine, per fresh mass; High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS), Shimadzu, LCMS-8050; Histidine, per fresh mass; Hydroxyproline, per fresh mass; hypoxia; intertidal; invasive species; Isoleucine, per fresh mass; Königshafen, Sylt, Germany; Laboratory; Laboratory experiment; Lactate, per fresh mass; Length; Leucine, per fresh mass; Lipid peroxidation, per fresh mass; Lysine, per fresh mass; Magallana gigas; Malate, per fresh mass; Mass; metabolism; metabolome; metabolomics; Methionine, per fresh mass; Methionine sulfoxide, per fresh mass; Multi-user microplate reader, Molecular devices, SpectraMax iD3; Origin; Ornithine, per fresh mass; oxidaitive stress; oxidative damage; Oxygen; Oyster; Phenylalanine, per fresh mass; Proline, per fresh mass; Protein carbonyl, per protein mass; Proteins per fresh mass; Sample ID; Sampling date/time, experiment; Serine, per fresh mass; Species, unique identification; Specimen identification; Succinate, per fresh mass; Tank number; Taurine, per fresh mass; Temperature; Threonine, per fresh mass; tidal cycle; Tissue type; Treatment: air exposure; Treatment: salinity; Treatment: temperature; Tryptophan, per fresh mass; Type of study; Tyrosine, per fresh mass; Valine, per fresh mass; Waddensea; Width |
| url | https://doi.org/10.1594/PANGAEA.980734 |