Nutrient limitation bioassays using natural populations of Gephyrocapsa huxleyi from the Gulf of Aqaba in contrasting seasons
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2025
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| _version_ | 1867168237553188864 |
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| author | Keuter, Sabine Frada, Miguel J Koplovitz, Gil Ben Yehouda, Maayan |
| author_facet | Keuter, Sabine Frada, Miguel J Koplovitz, Gil Ben Yehouda, Maayan |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | To disentangle the effect of macronutrient availability on cell-size in native populations of coccolithophores we surveyed the seasonality of the prevalent coccolithophores in the northern Red Sea and the Eastern Mediterranean and performed a series of incubations assays. For bioassays in stratified season and during winter mixing water samples were taken from 2 m depth off the pier of the IUI in Eilat (29.501735 N, 34.917801 E) and incubated at in situ temperature for 48 hours. 1 µM NaNO3 or/and 0.0625 µM of Na2 b-glycerophosphate were added to test for nutrient limitations in the Gulf of Aqaba. Scanning electron microscopy was used for coccolithophore enumeration and eukaryotic microalgae cells were identified and counted based on cell size scatter signals and red (chlorophyll) fluorescence using flow cytometry. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_983184 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Nutrient limitation bioassays using natural populations of Gephyrocapsa huxleyi from the Gulf of Aqaba in contrasting seasons Keuter, Sabine Frada, Miguel J Koplovitz, Gil Ben Yehouda, Maayan Cell density; Counts; DATE/TIME; DEPTH, water; Emiliania huxleyi; Event label; FERT; Fertilization experiment; Field experiment; Flow cytometry; Gephyrocapsa huxleyi; Gulf of Aqaba; Incubation duration; incubation experiment; IUI_pier_nutrients; Sample code/label; Scanning electron microscopy (SEM); Treatment; Volume; Water sample; WS To disentangle the effect of macronutrient availability on cell-size in native populations of coccolithophores we surveyed the seasonality of the prevalent coccolithophores in the northern Red Sea and the Eastern Mediterranean and performed a series of incubations assays. For bioassays in stratified season and during winter mixing water samples were taken from 2 m depth off the pier of the IUI in Eilat (29.501735 N, 34.917801 E) and incubated at in situ temperature for 48 hours. 1 µM NaNO3 or/and 0.0625 µM of Na2 b-glycerophosphate were added to test for nutrient limitations in the Gulf of Aqaba. Scanning electron microscopy was used for coccolithophore enumeration and eukaryotic microalgae cells were identified and counted based on cell size scatter signals and red (chlorophyll) fluorescence using flow cytometry. |
| title | Nutrient limitation bioassays using natural populations of Gephyrocapsa huxleyi from the Gulf of Aqaba in contrasting seasons |
| topic | Cell density; Counts; DATE/TIME; DEPTH, water; Emiliania huxleyi; Event label; FERT; Fertilization experiment; Field experiment; Flow cytometry; Gephyrocapsa huxleyi; Gulf of Aqaba; Incubation duration; incubation experiment; IUI_pier_nutrients; Sample code/label; Scanning electron microscopy (SEM); Treatment; Volume; Water sample; WS |
| url | https://doi.org/10.1594/PANGAEA.983184 |