Menthol-DCMU bleaching and re-inoculation of Symbiodiniaceae in the large benthic foraminifera Sorites orbiculus
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| Auteurs principaux: | , , , , , , , , |
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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2026
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| _version_ | 1867169521326882816 |
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| author | Timme, Lukas Theodor Henschen, Helena Puerto Rueda, Diana N Manda, Sneha Parkinson, John Everett Wild, Christian Abramovich, Sigal Morard, Raphael Schmidt, Christiane |
| author_facet | Timme, Lukas Theodor Henschen, Helena Puerto Rueda, Diana N Manda, Sneha Parkinson, John Everett Wild, Christian Abramovich, Sigal Morard, Raphael Schmidt, Christiane |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Sorites orbiculus specimens were collected on 26 July 2023 from the Gulf of Aqaba at the Interuniversity Institute for Marine Sciences in Eilat. In a controlled laboratory setting at the Leibniz Institute for Tropical Marine Research MAREE facility, these specimens underwent a bleaching process using 0.19 mmol L-1 menthol and 5 µmol L-1 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea (DCMU) over 27 days. Post-bleaching, the specimens were re-inoculated for 19 days with the Symbiodiniaceae strains Symbiodinium microadriaticum (strains CCMP2467 and KB8) and Fugacium kawagutii (strain F2) at a concentration of 1 × 10⁵ cells mL⁻¹. Subsequently, the specimens were cultivated for an additional 37 days to assess the stability of the newly established symbiotic associations. Throughout the experiment, physiological parameters were monitored using confocal laser scanning microscopy, pulse-amplitude modulated fluorometry, standard microscopy, motility assessments, and real-time quantitative polymerase chain reaction to confirm symbiont identity. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_984033 |
| institution | PANGAEA |
| language | en |
| publishDate | 2026 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Menthol-DCMU bleaching and re-inoculation of Symbiodiniaceae in the large benthic foraminifera Sorites orbiculus Timme, Lukas Theodor Henschen, Helena Puerto Rueda, Diana N Manda, Sneha Parkinson, John Everett Wild, Christian Abramovich, Sigal Morard, Raphael Schmidt, Christiane assisted evolution; CLSM; growth rate; Gulf of Aqaba; menthol-DCMU bleaching; motility; PAM fluorometry; qPCR; S_orbiculus_2023; Sampling by snorkeling; SNORKELING; symbiont switching Sorites orbiculus specimens were collected on 26 July 2023 from the Gulf of Aqaba at the Interuniversity Institute for Marine Sciences in Eilat. In a controlled laboratory setting at the Leibniz Institute for Tropical Marine Research MAREE facility, these specimens underwent a bleaching process using 0.19 mmol L-1 menthol and 5 µmol L-1 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea (DCMU) over 27 days. Post-bleaching, the specimens were re-inoculated for 19 days with the Symbiodiniaceae strains Symbiodinium microadriaticum (strains CCMP2467 and KB8) and Fugacium kawagutii (strain F2) at a concentration of 1 × 10⁵ cells mL⁻¹. Subsequently, the specimens were cultivated for an additional 37 days to assess the stability of the newly established symbiotic associations. Throughout the experiment, physiological parameters were monitored using confocal laser scanning microscopy, pulse-amplitude modulated fluorometry, standard microscopy, motility assessments, and real-time quantitative polymerase chain reaction to confirm symbiont identity. |
| title | Menthol-DCMU bleaching and re-inoculation of Symbiodiniaceae in the large benthic foraminifera Sorites orbiculus |
| topic | assisted evolution; CLSM; growth rate; Gulf of Aqaba; menthol-DCMU bleaching; motility; PAM fluorometry; qPCR; S_orbiculus_2023; Sampling by snorkeling; SNORKELING; symbiont switching |
| url | https://doi.org/10.1594/PANGAEA.984033 |