Confocal laser scanning microscopy monitoring re-inoculation of Symbiodiniaceae in the foraminifera Sorites orbiculus
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2026
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| _version_ | 1867172416160006144 |
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| author | Timme, Lukas Theodor Henschen, Helena Puerto Rueda, Diana N Manda, Sneha Parkinson, John Everett Wild, Christian Abramovich, Sigal Morard, Raphael Schmidt, Christiane |
| author_facet | Timme, Lukas Theodor Henschen, Helena Puerto Rueda, Diana N Manda, Sneha Parkinson, John Everett Wild, Christian Abramovich, Sigal Morard, Raphael Schmidt, Christiane |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Bleached Sorites orbiculus specimens were re-inoculated with Symbiodiniaceae strains in a laboratory experiment for 19 days, and the percentage of host area covered with symbionts was monitored with Confocal Laser Scanning Microscopy (CLSM). Specimens were placed in 6-well plates, with 2 samples containing 2 replicates per treatment / control. Treatments were exposed to Symbiodinium microadriaticum (strains KB8 and CCMP2467) and Fugacium kawagutii (strain F2) individually and collectively at a concentration of 1e5 cells ml-1. The medium was supplemented with a Nannochloropsis food mixture and exchanged twice weekly. Control groups included non-bleached specimens and bleached specimens maintained with or without 5 µmol L-1 DCMU in artificial seawater (ASW). Environmental conditions were maintained at 22 - 24°C temperature, 37.5 - 40.5‰ salinity, and 46 - 63 µmol photons cm-2 s-1 illumination under a 12:12 h light-dark cycle. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_984035 |
| institution | PANGAEA |
| language | en |
| publishDate | 2026 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Confocal laser scanning microscopy monitoring re-inoculation of Symbiodiniaceae in the foraminifera Sorites orbiculus Timme, Lukas Theodor Henschen, Helena Puerto Rueda, Diana N Manda, Sneha Parkinson, John Everett Wild, Christian Abramovich, Sigal Morard, Raphael Schmidt, Christiane Area; assisted evolution; CLSM; Coverage; DATE/TIME; Experimental treatment; Gulf of Aqaba; Method comment; S_orbiculus_2023; Sample ID; Sampling by snorkeling; SNORKELING; Specimen number; symbiont switching; Type of study Bleached Sorites orbiculus specimens were re-inoculated with Symbiodiniaceae strains in a laboratory experiment for 19 days, and the percentage of host area covered with symbionts was monitored with Confocal Laser Scanning Microscopy (CLSM). Specimens were placed in 6-well plates, with 2 samples containing 2 replicates per treatment / control. Treatments were exposed to Symbiodinium microadriaticum (strains KB8 and CCMP2467) and Fugacium kawagutii (strain F2) individually and collectively at a concentration of 1e5 cells ml-1. The medium was supplemented with a Nannochloropsis food mixture and exchanged twice weekly. Control groups included non-bleached specimens and bleached specimens maintained with or without 5 µmol L-1 DCMU in artificial seawater (ASW). Environmental conditions were maintained at 22 - 24°C temperature, 37.5 - 40.5‰ salinity, and 46 - 63 µmol photons cm-2 s-1 illumination under a 12:12 h light-dark cycle. |
| title | Confocal laser scanning microscopy monitoring re-inoculation of Symbiodiniaceae in the foraminifera Sorites orbiculus |
| topic | Area; assisted evolution; CLSM; Coverage; DATE/TIME; Experimental treatment; Gulf of Aqaba; Method comment; S_orbiculus_2023; Sample ID; Sampling by snorkeling; SNORKELING; Specimen number; symbiont switching; Type of study |
| url | https://doi.org/10.1594/PANGAEA.984035 |