PAM fluorometry monitoring symbiosis stability of Symbiodiniaceae in the foraminifera Sorites orbiculus

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Hauptverfasser: Timme, Lukas Theodor, Henschen, Helena, Puerto Rueda, Diana N, Manda, Sneha, Parkinson, John Everett, Wild, Christian, Abramovich, Sigal, Morard, Raphael, Schmidt, Christiane
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Sprache:en
Veröffentlicht: PANGAEA 2026
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author Timme, Lukas Theodor
Henschen, Helena
Puerto Rueda, Diana N
Manda, Sneha
Parkinson, John Everett
Wild, Christian
Abramovich, Sigal
Morard, Raphael
Schmidt, Christiane
author_facet Timme, Lukas Theodor
Henschen, Helena
Puerto Rueda, Diana N
Manda, Sneha
Parkinson, John Everett
Wild, Christian
Abramovich, Sigal
Morard, Raphael
Schmidt, Christiane
collection Datos científicos de ciencias marinas y ambientales
contents The photosystem II efficiency of the symbiotic algae of Sorites orbiculus was measured with PAM fluorometry in an experiment, assessing the long-term stability of newly established symbiotic associations. Therefore, specimens were cultivated for 14 days following menthol-DCMU bleaching and re-inoculation with Symbiodinium microadriaticum (strains KB8 and CCMP2467) and Fugacium kawagutii (strain F2) individually and collectively. The final re-inoculation measurement also served as the initial stability measurement. Accordingly, this measurement is included in both data tables and is provided here under the experimental treatment label "re-inoculation." Specimens were cultivated in 6-well plates, with 12 samples containing 4 replicates and 2 samples containing 2 replicates per treatment / control. The medium, supplemented with a Nannochloropsis food mixture, was exchanged twice weekly. Control groups included non-bleached specimens and bleached specimens maintained with or without 5 µmol L-1 DCMU in artificial seawater (ASW). Environmental conditions were maintained at 22 - 24°C temperature, 37.5 - 40.5‰ salinity, and 46 - 63 µmol photons cm-2 s-1 illumination under a 12:12 h light-dark cycle.
format Dataset Open Access
id pangaea_https___doi_org_10_1594_PANGAEA_984043
institution PANGAEA
language en
publishDate 2026
publisher PANGAEA
record_format pangaea
spellingShingle PAM fluorometry monitoring symbiosis stability of Symbiodiniaceae in the foraminifera Sorites orbiculus
Timme, Lukas Theodor
Henschen, Helena
Puerto Rueda, Diana N
Manda, Sneha
Parkinson, John Everett
Wild, Christian
Abramovich, Sigal
Morard, Raphael
Schmidt, Christiane
assisted evolution; DATE/TIME; Effective quantum yield of photosystem II; Experimental treatment; Fluorescence; Gulf of Aqaba; Maximum quantum yield of photosystem II; Method comment; PAM fluorometry; S_orbiculus_2023; Sample ID; Sampling by snorkeling; SNORKELING; symbiont switching; Type of study
The photosystem II efficiency of the symbiotic algae of Sorites orbiculus was measured with PAM fluorometry in an experiment, assessing the long-term stability of newly established symbiotic associations. Therefore, specimens were cultivated for 14 days following menthol-DCMU bleaching and re-inoculation with Symbiodinium microadriaticum (strains KB8 and CCMP2467) and Fugacium kawagutii (strain F2) individually and collectively. The final re-inoculation measurement also served as the initial stability measurement. Accordingly, this measurement is included in both data tables and is provided here under the experimental treatment label "re-inoculation." Specimens were cultivated in 6-well plates, with 12 samples containing 4 replicates and 2 samples containing 2 replicates per treatment / control. The medium, supplemented with a Nannochloropsis food mixture, was exchanged twice weekly. Control groups included non-bleached specimens and bleached specimens maintained with or without 5 µmol L-1 DCMU in artificial seawater (ASW). Environmental conditions were maintained at 22 - 24°C temperature, 37.5 - 40.5‰ salinity, and 46 - 63 µmol photons cm-2 s-1 illumination under a 12:12 h light-dark cycle.
title PAM fluorometry monitoring symbiosis stability of Symbiodiniaceae in the foraminifera Sorites orbiculus
topic assisted evolution; DATE/TIME; Effective quantum yield of photosystem II; Experimental treatment; Fluorescence; Gulf of Aqaba; Maximum quantum yield of photosystem II; Method comment; PAM fluorometry; S_orbiculus_2023; Sample ID; Sampling by snorkeling; SNORKELING; symbiont switching; Type of study
url https://doi.org/10.1594/PANGAEA.984043