CFU counts of Vibrio spp. on seaweeds and water column at 16°C and 20°C

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Autores principales: Wilson, Sidney, Saha, Mahasweta
Formato: Dataset Open Access
Lenguaje:en
Publicado: PANGAEA 2025
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author Wilson, Sidney
Saha, Mahasweta
author_facet Wilson, Sidney
Saha, Mahasweta
collection Datos científicos de ciencias marinas y ambientales
contents We investigated the impact of elevated water temperatures on the abundances of pathogenic Vibrio species associated with seaweeds and coastal waters. Three seaweed species - Fucus serratus, Palmaria palmata, and Ulva spp.- were collected from Plymouth Sound, United Kingdom, and used in a mesocosm experiment conducted at Plymouth Marine Laboratory in June 2024. The samples were exposed to two temperature treatments (16 °C and 20 °C) for seven days each to simulate warming ocean conditions. After the seven-day treatments, microbial suspensions (referred to as microbial soups) were prepared from each seaweed replicate and corresponding water column sample. These suspensions were filtered and incubated on ChromAgar Vibrio chromogenic media plates for 72 hours to selectively identify and differentiate Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio vulnificus/cholerae. Colony-forming units (CFUs) were counted for each replicate, providing quantitative data on the response of these Vibrio species to elevated temperatures.
format Dataset Open Access
id pangaea_https___doi_org_10_1594_PANGAEA_984222
institution PANGAEA
language en
publishDate 2025
publisher PANGAEA
record_format pangaea
spellingShingle CFU counts of Vibrio spp. on seaweeds and water column at 16°C and 20°C
Wilson, Sidney
Saha, Mahasweta
Counted; HAND; Laboratory experiment; mesocosm experiment; PlymouthSound_20240603; Replicate; Sample type; Sampling by hand; Treatment: temperature; Type of study; United Kingdom; Vibrio alginolyticus, abundance in colony forming units; Vibrio parahaemolyticus, abundance in colony forming units; Vibrio vulnificus and Vibrio cholerae, abundance in colony forming units
We investigated the impact of elevated water temperatures on the abundances of pathogenic Vibrio species associated with seaweeds and coastal waters. Three seaweed species - Fucus serratus, Palmaria palmata, and Ulva spp.- were collected from Plymouth Sound, United Kingdom, and used in a mesocosm experiment conducted at Plymouth Marine Laboratory in June 2024. The samples were exposed to two temperature treatments (16 °C and 20 °C) for seven days each to simulate warming ocean conditions. After the seven-day treatments, microbial suspensions (referred to as microbial soups) were prepared from each seaweed replicate and corresponding water column sample. These suspensions were filtered and incubated on ChromAgar Vibrio chromogenic media plates for 72 hours to selectively identify and differentiate Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio vulnificus/cholerae. Colony-forming units (CFUs) were counted for each replicate, providing quantitative data on the response of these Vibrio species to elevated temperatures.
title CFU counts of Vibrio spp. on seaweeds and water column at 16°C and 20°C
topic Counted; HAND; Laboratory experiment; mesocosm experiment; PlymouthSound_20240603; Replicate; Sample type; Sampling by hand; Treatment: temperature; Type of study; United Kingdom; Vibrio alginolyticus, abundance in colony forming units; Vibrio parahaemolyticus, abundance in colony forming units; Vibrio vulnificus and Vibrio cholerae, abundance in colony forming units
url https://doi.org/10.1594/PANGAEA.984222