Excitation-emission matrices produced by custom-built high-pressure cuvette system
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| Autores principales: | , , |
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
| Publicado: |
PANGAEA
2025
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| _version_ | 1867168238296629248 |
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| author | Papadimitraki, Maria Wünsch, Urban Glud, Ronnie N |
| author_facet | Papadimitraki, Maria Wünsch, Urban Glud, Ronnie N |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | A custom high-pressure cuvette system was developed, capable of operating at pressures up to 110 MPa to investigate pressure-dependent effects on enzymatic activity and biogeochemical element cycling in the deep ocean. This system features three sapphire windows for real-time absorption and fluorescence measurements, as well as temperature control. This dataset contains the maximum-normalized fluorescence measurements obtained using this system, for assessing the enzymatic activity of extracellular chitinase produced by a piezo-sensitive Vibrio anguillarum (PF430-3, originally isolated in Chile) strain. Specifically, the dataset includes excitation-emission matrices (EEMs) for the substrate 4-MUF-NAG, the fluorophore 4-MUF and the combined signal from 4-MUF-NAG in 30% medium along with cleaved 4-MUF in the presence of chitinase enzymes. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_984327 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Excitation-emission matrices produced by custom-built high-pressure cuvette system Papadimitraki, Maria Wünsch, Urban Glud, Ronnie N Bacteria; Date/time end, experiment; Date/time start, experiment; Deep sea; enzyme activity; Fluorescence, substrate, normalized; fluorescence measurement; hydrostatic pressure; Laboratory experiment; method; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Substrate, concentration; Substrate type; Treatment: pressure; Type of study; Wavelength, emission; Wavelength, excitation A custom high-pressure cuvette system was developed, capable of operating at pressures up to 110 MPa to investigate pressure-dependent effects on enzymatic activity and biogeochemical element cycling in the deep ocean. This system features three sapphire windows for real-time absorption and fluorescence measurements, as well as temperature control. This dataset contains the maximum-normalized fluorescence measurements obtained using this system, for assessing the enzymatic activity of extracellular chitinase produced by a piezo-sensitive Vibrio anguillarum (PF430-3, originally isolated in Chile) strain. Specifically, the dataset includes excitation-emission matrices (EEMs) for the substrate 4-MUF-NAG, the fluorophore 4-MUF and the combined signal from 4-MUF-NAG in 30% medium along with cleaved 4-MUF in the presence of chitinase enzymes. |
| title | Excitation-emission matrices produced by custom-built high-pressure cuvette system |
| topic | Bacteria; Date/time end, experiment; Date/time start, experiment; Deep sea; enzyme activity; Fluorescence, substrate, normalized; fluorescence measurement; hydrostatic pressure; Laboratory experiment; method; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Substrate, concentration; Substrate type; Treatment: pressure; Type of study; Wavelength, emission; Wavelength, excitation |
| url | https://doi.org/10.1594/PANGAEA.984327 |