Histological and multi-biomarker analyses in Limanda limanda collected from shipwrecks in the North Sea to assess the biological effects of old World War munitions
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2025
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| _version_ | 1867171053972750336 |
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| author | Schuster, Romina Marietta Binder, Franziska Isabelle De Rijcke, Maarten Van Haelst, Sven Andresen, Katrine Juul Brenner, Matthias |
| author_facet | Schuster, Romina Marietta Binder, Franziska Isabelle De Rijcke, Maarten Van Haelst, Sven Andresen, Katrine Juul Brenner, Matthias |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | This dataset documents field investigations on the biological effects of legacy World War I munitions on marine organisms, with a focus on shipwreck sites in the North Sea. Three historically well-documented wrecks were selected: the light cruiser SMS Mainz, the minelayer SMS Ariadne, and the submarine UC30. These wrecks were chosen based on detailed archival information regarding their sinking circumstances and cargo, their unambiguous identification, and their accessibility for scientific diving operations. As a munition-free control, a reference area outside known wreck fields was sampled (Naturschutzgebiet Borkum Riffgrund). The flatfish Limanda limanda (dab) was selected as a sentinel species. Sampling was conducted during several cruises with the research vessel Heincke (HE 573, April 2021 – SMS Mainz; HE 596, April 2022 – UC30 and SMS Ariadne; HE 607, September 2022 – UC30; HE 613, February 2023 – SMS Ariadne) and with the Uthörn (May 2022 – reference site). Fish were caught using bottom trawls deployed as close as possible to the wreck structures. Captured fish were transferred to seawater tanks prior to dissection. Each specimen was measured, weighed, and assessed biometrically to calculate condition factors as indicators of general health. Subsequent processing included macroscopic examination of livers for pathological alterations (e.g., fatty liver, nodules, bile stasis, tumor formation). Liver tissue was partitioned for histological analysis (H&E staining after methacrylate embedding), histochemical biomarker assays (lipofuscin accumulation, neutral lipids, lysosomal membrane stability), and biochemical measurements. Enzyme activities of catalase (CAT) and glutathione S-transferase (GST) were quantified in liver and muscle homogenates using spectrophotometric assays and normalized to protein content. Both otoliths were extracted for age determination, dried, embedded in resin, and microscopically examined under variable light intensities to identify annual growth rings. The resulting dataset integrates biometric data, liver pathology, histological and histochemical biomarkers, enzyme activity profiles, and otolith-based age determinations from dabs collected at munition-affected shipwrecks and a reference site. It provides a standardized and comprehensive resource to evaluate the potential toxicological effects of World War I munitions on marine fish populations in the North Sea. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_984858 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Histological and multi-biomarker analyses in Limanda limanda collected from shipwrecks in the North Sea to assess the biological effects of old World War munitions Schuster, Romina Marietta Binder, Franziska Isabelle De Rijcke, Maarten Van Haelst, Sven Andresen, Katrine Juul Brenner, Matthias Biomarkers; Bottom trawl; BT; Catalase activity, unit per protein mass; Common dab; CTD; DATE/TIME; DEPTH, water; Event label; Family; Glutathione S-transferase activity, unit per protein mass; HE573; HE573_17-1; HE596; HE596_18-1; HE596_8-1; HE607; HE607_1-7; HE613; HE613_1-5; Heincke; Histology; Image analysis; LATITUDE; Limanda limanda, age; Limanda limanda, sex; Limanda limanda, total length; Limanda limanda, wet mass; Liver, color; Location; LONGITUDE; Lysosomal area enriched with lipofuscin; Lysosomal area enriched with neutral lipids; Lysosomal membranes stability; Measuring board; Microplate reader; Microscopic image analysis; munition; North Sea; North Sea Interreg REMARCO; North Sea Wrecks; NSW; NS-Wrecks, NSW; REMARCO; Sample ID; Scale; ship wrecks; Site; Species, unique identification; Station label; UT05/2022; Uthörn; Uthörn_20220518; Uthörn_20220518-3; Visual inspection; world war I This dataset documents field investigations on the biological effects of legacy World War I munitions on marine organisms, with a focus on shipwreck sites in the North Sea. Three historically well-documented wrecks were selected: the light cruiser SMS Mainz, the minelayer SMS Ariadne, and the submarine UC30. These wrecks were chosen based on detailed archival information regarding their sinking circumstances and cargo, their unambiguous identification, and their accessibility for scientific diving operations. As a munition-free control, a reference area outside known wreck fields was sampled (Naturschutzgebiet Borkum Riffgrund). The flatfish Limanda limanda (dab) was selected as a sentinel species. Sampling was conducted during several cruises with the research vessel Heincke (HE 573, April 2021 – SMS Mainz; HE 596, April 2022 – UC30 and SMS Ariadne; HE 607, September 2022 – UC30; HE 613, February 2023 – SMS Ariadne) and with the Uthörn (May 2022 – reference site). Fish were caught using bottom trawls deployed as close as possible to the wreck structures. Captured fish were transferred to seawater tanks prior to dissection. Each specimen was measured, weighed, and assessed biometrically to calculate condition factors as indicators of general health. Subsequent processing included macroscopic examination of livers for pathological alterations (e.g., fatty liver, nodules, bile stasis, tumor formation). Liver tissue was partitioned for histological analysis (H&E staining after methacrylate embedding), histochemical biomarker assays (lipofuscin accumulation, neutral lipids, lysosomal membrane stability), and biochemical measurements. Enzyme activities of catalase (CAT) and glutathione S-transferase (GST) were quantified in liver and muscle homogenates using spectrophotometric assays and normalized to protein content. Both otoliths were extracted for age determination, dried, embedded in resin, and microscopically examined under variable light intensities to identify annual growth rings. The resulting dataset integrates biometric data, liver pathology, histological and histochemical biomarkers, enzyme activity profiles, and otolith-based age determinations from dabs collected at munition-affected shipwrecks and a reference site. It provides a standardized and comprehensive resource to evaluate the potential toxicological effects of World War I munitions on marine fish populations in the North Sea. |
| title | Histological and multi-biomarker analyses in Limanda limanda collected from shipwrecks in the North Sea to assess the biological effects of old World War munitions |
| topic | Biomarkers; Bottom trawl; BT; Catalase activity, unit per protein mass; Common dab; CTD; DATE/TIME; DEPTH, water; Event label; Family; Glutathione S-transferase activity, unit per protein mass; HE573; HE573_17-1; HE596; HE596_18-1; HE596_8-1; HE607; HE607_1-7; HE613; HE613_1-5; Heincke; Histology; Image analysis; LATITUDE; Limanda limanda, age; Limanda limanda, sex; Limanda limanda, total length; Limanda limanda, wet mass; Liver, color; Location; LONGITUDE; Lysosomal area enriched with lipofuscin; Lysosomal area enriched with neutral lipids; Lysosomal membranes stability; Measuring board; Microplate reader; Microscopic image analysis; munition; North Sea; North Sea Interreg REMARCO; North Sea Wrecks; NSW; NS-Wrecks, NSW; REMARCO; Sample ID; Scale; ship wrecks; Site; Species, unique identification; Station label; UT05/2022; Uthörn; Uthörn_20220518; Uthörn_20220518-3; Visual inspection; world war I |
| url | https://doi.org/10.1594/PANGAEA.984858 |