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Microbiological investigation (qPCR) on sediment samples from thermokarst terrain in the continuous permafrost zone of Arctic Alaska

Fuente: PANGAEA

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Bibliographic Details
Main Authors:	Seemann, Fabian, Liebner, Susanne, Saborowski, Anke, Jenrich, Maren, Strauss, Jens
Format:	Dataset Open Access
Language:	en
Published:	PANGAEA 2025
Subjects:	16S rRNA gene, abundance, per dry mass; AK-Land_2022_NorthSlope; AK-Land_2022_NorthSlope_BRW22_DLB_001; AK-Land_2022_NorthSlope_BRW22_ETL_001; AK-Land_2022_NorthSlope_BRW22_UL_001; AK-Land_2022_NorthSlope_BRW22_WTL_001; AK-Land_2024_NWAlaska_spring; AK-Land_2024_NWAlaska_spring_BAL24_T0_DLB_001; AK-Land_2024_NWAlaska_spring_BAL24_T0_L_002; AK-Land_2024_NWAlaska_spring_BAL24_T0_UL_001; Alaskan North Slope; Area/locality; AWI_Perma; AWI Arctic Land Expedition; Core; DEPTH, sediment/rock; Depth, sediment/rock, bottom/maximum; Depth, sediment/rock, top/minimum; Event label; Functional gene dissimilatory sulfite reductase genes, presence, per dry mass; Methane oxygenase, pmoA gene, abundance, per dry mass; Methanogene mcrA copies, per dry mass; methanogens; methanotrophs; microbial abundance; Permafrost Research; Perma-X Lagoons Alaska 2022; PUC; Push corer; qPCR; Sample code/label; Sediment; SIPREC; SIPRE corer; Soil water content; Sulfate reducing bacteria; SYBRGreen quantitative PCR (qPCR) assay using the KAPA SYBRFAST qPCR Master Mix (Sigma-Aldrich, Germany) on CFX96 real-time thermal cycler (Bio-Rad Laboratories Inc., United States); thermokarst; Vibra-corer
Online Access:	Acceder al recurso
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Internet

https://doi.org/10.1594/PANGAEA.985099