Growth, respiration rates and energetic reserves from a long-term (12-months) multiple driver aquarium experiment with the cold-water coral Desmophyllum pertusum
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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2025
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| _version_ | 1867171902095622144 |
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| author | Beck, Kristina K Roberts, J Murray Burns, Zoe Easton, Blair Kaye, Sarah Peña Fernández, Marta Wolfram, Uwe Hennige, Sebastian |
| author_facet | Beck, Kristina K Roberts, J Murray Burns, Zoe Easton, Blair Kaye, Sarah Peña Fernández, Marta Wolfram, Uwe Hennige, Sebastian |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | We conducted a long-term (12 months) multiple driver aquarium experiment under future environmental conditions at St Abbs Marine Station (UK) with the cold-water coral Lophelia pertusa (syn. Desmophyllum pertusum) sampled from Tisler Reef (Skagerrak). The experiment consisted of four different treatments to investigate the combined effect of ocean acidification, warming, deoxygenation and food limitation on their physiology: 1) control (9 °C, pH 8.1, 100 % oxygen, 100 % food availability), 2) multiple stressor with high feeding (HF; 12 °C, pH 7.7, 90 % oxygen, 100 % food availability), 3) multiple stressor with low feeding (LF; 12 °C, pH 7.7, 90 % oxygen, 50 % food availability) and 4) reduced oxygen (9 °C, pH 8.1, 90 % oxygen, 100 % food availability). Every treatment consisted of three replicate tanks with four live corals (12 in total). Water parameters (temperature, salinity, pH and oxygen concentration) were measured five times per week in every coral tank. The physiological parameters (survival, growth and respiration rate) were determined after 1.5, 3, 4.5, 6, 7.5, 9, 10.5 and 12 months of the experiment. Growth rates were measured using the buoyant weighing technique (Jokiel et al. 1978) with a high precision electronic balance (Pioneer Plus PA224C, OHAUS; precision: 0.1 mg) every 1.5 months. The skeletal dry mass of each coral was calculated from its wet mass and by taking into account the densities of seawater (1.023 ± 0.008 g cm-3) and L. pertusa skeletons (2.747 g cm-3; Naumann et al., 2014). Respiration rates were measured in closed-cell incubations vials in a water bath at the start of the experiment and every 1.5 months. In addition, the energetic reserves (protein, carbohydrate and lipid concentration) of six coral samples per treatment were analysed after 3, 6, 9 and 12 months. The protein, carbohydrate and lipid concentrations were then converted to kilojoules (kJ; protein: 23.9 kJ g-1, carbohydrate: 17.5 kJ g-1, lipid: 39.5 kJ g-1; Gnaiger & Bitterlich, 1984). |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_987327 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Growth, respiration rates and energetic reserves from a long-term (12-months) multiple driver aquarium experiment with the cold-water coral Desmophyllum pertusum Beck, Kristina K Roberts, J Murray Burns, Zoe Easton, Blair Kaye, Sarah Peña Fernández, Marta Wolfram, Uwe Hennige, Sebastian Ash free dry mass; Buoyant weighing technique according to Jokiel et al. (1978); calcification; Calculated; Carbohydrates, per ash free dry mass; Climate change; Colorimetric lipid quantification, microplate format (Cheng et al. 2011); Deoxygenation; Desmophyllum_pertusum_Sampling_Area_and_Period; Desmophyllum pertusum; Desmophyllum pertusum, dry mass; Desmophyllum pertusum, growth rate; Desmophyllum pertusum, respiration rate, oxygen, per polyp; Duration; energy reserves; Fibre optic oxygen and temperature probes (OXY-4 SMA, POF, PSt3, Pt100, PreSens); food limitation; growth; iAtlantic; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; Lipids, per ash free dry mass; Ocean acidification; Phenol-sulfuric acid assay according to Dubois et al. (1956); Proteins, per ash free dry mass; Quantification of total protein according to Bradford (1976); Remote operated vehicle; Respiration; Respiration rate, oxygen, per ash free dry mass; ROV; Species, unique identification (Semantic URI); Species, unique identification (URI); Specimen identification; Tank number; Tisler Reef, Skagerrak; Total energy reserve, per ash free dry mass; Treatment; Type of study; warming We conducted a long-term (12 months) multiple driver aquarium experiment under future environmental conditions at St Abbs Marine Station (UK) with the cold-water coral Lophelia pertusa (syn. Desmophyllum pertusum) sampled from Tisler Reef (Skagerrak). The experiment consisted of four different treatments to investigate the combined effect of ocean acidification, warming, deoxygenation and food limitation on their physiology: 1) control (9 °C, pH 8.1, 100 % oxygen, 100 % food availability), 2) multiple stressor with high feeding (HF; 12 °C, pH 7.7, 90 % oxygen, 100 % food availability), 3) multiple stressor with low feeding (LF; 12 °C, pH 7.7, 90 % oxygen, 50 % food availability) and 4) reduced oxygen (9 °C, pH 8.1, 90 % oxygen, 100 % food availability). Every treatment consisted of three replicate tanks with four live corals (12 in total). Water parameters (temperature, salinity, pH and oxygen concentration) were measured five times per week in every coral tank. The physiological parameters (survival, growth and respiration rate) were determined after 1.5, 3, 4.5, 6, 7.5, 9, 10.5 and 12 months of the experiment. Growth rates were measured using the buoyant weighing technique (Jokiel et al. 1978) with a high precision electronic balance (Pioneer Plus PA224C, OHAUS; precision: 0.1 mg) every 1.5 months. The skeletal dry mass of each coral was calculated from its wet mass and by taking into account the densities of seawater (1.023 ± 0.008 g cm-3) and L. pertusa skeletons (2.747 g cm-3; Naumann et al., 2014). Respiration rates were measured in closed-cell incubations vials in a water bath at the start of the experiment and every 1.5 months. In addition, the energetic reserves (protein, carbohydrate and lipid concentration) of six coral samples per treatment were analysed after 3, 6, 9 and 12 months. The protein, carbohydrate and lipid concentrations were then converted to kilojoules (kJ; protein: 23.9 kJ g-1, carbohydrate: 17.5 kJ g-1, lipid: 39.5 kJ g-1; Gnaiger & Bitterlich, 1984). |
| title | Growth, respiration rates and energetic reserves from a long-term (12-months) multiple driver aquarium experiment with the cold-water coral Desmophyllum pertusum |
| topic | Ash free dry mass; Buoyant weighing technique according to Jokiel et al. (1978); calcification; Calculated; Carbohydrates, per ash free dry mass; Climate change; Colorimetric lipid quantification, microplate format (Cheng et al. 2011); Deoxygenation; Desmophyllum_pertusum_Sampling_Area_and_Period; Desmophyllum pertusum; Desmophyllum pertusum, dry mass; Desmophyllum pertusum, growth rate; Desmophyllum pertusum, respiration rate, oxygen, per polyp; Duration; energy reserves; Fibre optic oxygen and temperature probes (OXY-4 SMA, POF, PSt3, Pt100, PreSens); food limitation; growth; iAtlantic; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; Lipids, per ash free dry mass; Ocean acidification; Phenol-sulfuric acid assay according to Dubois et al. (1956); Proteins, per ash free dry mass; Quantification of total protein according to Bradford (1976); Remote operated vehicle; Respiration; Respiration rate, oxygen, per ash free dry mass; ROV; Species, unique identification (Semantic URI); Species, unique identification (URI); Specimen identification; Tank number; Tisler Reef, Skagerrak; Total energy reserve, per ash free dry mass; Treatment; Type of study; warming |
| url | https://doi.org/10.1594/PANGAEA.987327 |