Relative proportions of fatty acids in Melosira assemblages, and subsequently isolated unialgal cultures collected in June 2023 during the RV Kronprins Haakon cruise 2023-1 in Fram Strait
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| Natura: | Dataset Open Access |
| Lingua: | en |
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PANGAEA
2025
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| _version_ | 1867168241557700608 |
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| author | Leu, Eva Šupraha, Luka Graeve, Martin Fahl, Kirsten Müller, Anja Müller, Juliane Lunden, Jonas Woll, Matthias Kohlbach, Doreen |
| author_facet | Leu, Eva Šupraha, Luka Graeve, Martin Fahl, Kirsten Müller, Anja Müller, Juliane Lunden, Jonas Woll, Matthias Kohlbach, Doreen |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | During the AO2023-1 expedition (1-21 June 2023), Melosira assemblages were collected using a handpump at a sampling site in Fram Strait. In addition to performing physiological measurements and filtering the material for trophic markers, living material was brought back to Oslo and two strains were established as unialgal cultures. The cultures are maintained in artificial seawater-based L1 medium at 4°C and 25 µmol m-2 s-1 light with a 16:8 L:D cycle. Lipids were extracted from freeze-dried samples following the method of Folch et al. (1957), using a dichloromethane/methanol mixture (2:1, v/v) in a sonication bath. The extracted lipids were converted to fatty acid methyl esters and analyzed by gas chromatography (Agilent 6890N Network GC, Agilent Technologies) equipped with a DB-FFAP capillary column (30 m length, 0.25 mm internal diameter, 0.25 μm film thickness), split injection, and a flame ionization detector. The temperature program ranged from 160°C to 240°C. Fatty acid analysis was conducted to complement datasets on fatty acid stable isotope compositions and sterol proportions. Collectively, the datasets enable a detailed characterization of the biochemical composition of different Melosira arctica-dominated algal assemblages from the Arctic Ocean, and a comparison with a unialgal culture of this important species. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_987819 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Relative proportions of fatty acids in Melosira assemblages, and subsequently isolated unialgal cultures collected in June 2023 during the RV Kronprins Haakon cruise 2023-1 in Fram Strait Leu, Eva Šupraha, Luka Graeve, Martin Fahl, Kirsten Müller, Anja Müller, Juliane Lunden, Jonas Woll, Matthias Kohlbach, Doreen (E,7R,11R)-3,7,11,15-tetramethylhexadec-2-en-1-ol; 11,14,17-Eicosatrienoic acid of total fatty acids; 11-Hexadecenoic acid of total fatty acids; 11-Icosenoic acid of total fatty acids; 11-Octadecenoic acid of total fatty acids; 13,16-Docosadienoic acid of total fatty acids; 13-Docosenoic acid of total fatty acids; 13-Icosenoic acid of total fatty acids; 13-Octadecenoic acid of total fatty acids; 15-Tetracosenoic acid of total fatty acids; 17-Docosenoic acid of total fatty acids; 2024007009; 4,7,10,13,16,19-Docosahexaenoic acid of total fatty acids; 5,8,11,14,17-Eicosapentaenoic acidof total fatty acids; 5,8,11,14-Eicosatetraenoic acid of total fatty acids; 6,9,12,15-Hexadecatetraenoic acid of total fatty acids; 6,9,12,15-Octadecatetraenoic acid of total fatty acids; 6,9,12-Hexadecatrienoic acid of total fatty acids; 7,10,13,16,19-Docosapentaenoic acid of total fatty acids; 7,10,13,16-Docosatetraenoic acid of total fatty acids; 8,11,14,17-Eicosatetraenoic acid of total fatty acids; 9,12-Hexadecadienoic acid of total fatty acids; 9,12-Octadecadienoic acid of total fatty acids; 9-Hexadecenoic acid of total fatty acids; 9-Octadecenoic acid of total fatty acids; 9-Tetradecenoic acid of total fatty acids; AO2023-1; AO2023-1_MA1; Arctic microalgae; Date/time end, experiment; Date/time start, experiment; Decanoic acid of total fatty acids; DEPTH, water; Depth comment; Docosanoic acid of total fatty acids; Docosatetraenoic acid 22:4(n-3) of total fatty acids; Event label; Experimental treatment; fatty acids; Fram Strait; Genus, unique identification; Genus, unique identification (Semantic URI); Genus, unique identification (URI); Hand pump; Heptadecanoic acid of total fatty acids; Hexadecanoic acid of total fatty acids; HP; Icosanoic acid of total fatty acids; iso-Pentadecanoic acid of total fatty acids; Kronprins Haakon; Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N; Melosira; Octadecanoic acid of total fatty acids; Pentadecanoic acid of total fatty acids; Reference in WoRMS; Sample code/label; Standard cultivation; Station label; Tetracosenoic acid 24:1(n-11) of total fatty acids; Tetradecanoic acid of total fatty acids; Treatment: nitrate; Treatment: phosphate; Treatment: salinity; Treatment: silicate; Treatment: temperature; trophic markers; Type of study; Unialgal cultures cultivation; WoRMS During the AO2023-1 expedition (1-21 June 2023), Melosira assemblages were collected using a handpump at a sampling site in Fram Strait. In addition to performing physiological measurements and filtering the material for trophic markers, living material was brought back to Oslo and two strains were established as unialgal cultures. The cultures are maintained in artificial seawater-based L1 medium at 4°C and 25 µmol m-2 s-1 light with a 16:8 L:D cycle. Lipids were extracted from freeze-dried samples following the method of Folch et al. (1957), using a dichloromethane/methanol mixture (2:1, v/v) in a sonication bath. The extracted lipids were converted to fatty acid methyl esters and analyzed by gas chromatography (Agilent 6890N Network GC, Agilent Technologies) equipped with a DB-FFAP capillary column (30 m length, 0.25 mm internal diameter, 0.25 μm film thickness), split injection, and a flame ionization detector. The temperature program ranged from 160°C to 240°C. Fatty acid analysis was conducted to complement datasets on fatty acid stable isotope compositions and sterol proportions. Collectively, the datasets enable a detailed characterization of the biochemical composition of different Melosira arctica-dominated algal assemblages from the Arctic Ocean, and a comparison with a unialgal culture of this important species. |
| title | Relative proportions of fatty acids in Melosira assemblages, and subsequently isolated unialgal cultures collected in June 2023 during the RV Kronprins Haakon cruise 2023-1 in Fram Strait |
| topic | (E,7R,11R)-3,7,11,15-tetramethylhexadec-2-en-1-ol; 11,14,17-Eicosatrienoic acid of total fatty acids; 11-Hexadecenoic acid of total fatty acids; 11-Icosenoic acid of total fatty acids; 11-Octadecenoic acid of total fatty acids; 13,16-Docosadienoic acid of total fatty acids; 13-Docosenoic acid of total fatty acids; 13-Icosenoic acid of total fatty acids; 13-Octadecenoic acid of total fatty acids; 15-Tetracosenoic acid of total fatty acids; 17-Docosenoic acid of total fatty acids; 2024007009; 4,7,10,13,16,19-Docosahexaenoic acid of total fatty acids; 5,8,11,14,17-Eicosapentaenoic acidof total fatty acids; 5,8,11,14-Eicosatetraenoic acid of total fatty acids; 6,9,12,15-Hexadecatetraenoic acid of total fatty acids; 6,9,12,15-Octadecatetraenoic acid of total fatty acids; 6,9,12-Hexadecatrienoic acid of total fatty acids; 7,10,13,16,19-Docosapentaenoic acid of total fatty acids; 7,10,13,16-Docosatetraenoic acid of total fatty acids; 8,11,14,17-Eicosatetraenoic acid of total fatty acids; 9,12-Hexadecadienoic acid of total fatty acids; 9,12-Octadecadienoic acid of total fatty acids; 9-Hexadecenoic acid of total fatty acids; 9-Octadecenoic acid of total fatty acids; 9-Tetradecenoic acid of total fatty acids; AO2023-1; AO2023-1_MA1; Arctic microalgae; Date/time end, experiment; Date/time start, experiment; Decanoic acid of total fatty acids; DEPTH, water; Depth comment; Docosanoic acid of total fatty acids; Docosatetraenoic acid 22:4(n-3) of total fatty acids; Event label; Experimental treatment; fatty acids; Fram Strait; Genus, unique identification; Genus, unique identification (Semantic URI); Genus, unique identification (URI); Hand pump; Heptadecanoic acid of total fatty acids; Hexadecanoic acid of total fatty acids; HP; Icosanoic acid of total fatty acids; iso-Pentadecanoic acid of total fatty acids; Kronprins Haakon; Lipid extraction according to Folch et al. (1957); followed by Gas chromatograph, Agilent, 6890N; Melosira; Octadecanoic acid of total fatty acids; Pentadecanoic acid of total fatty acids; Reference in WoRMS; Sample code/label; Standard cultivation; Station label; Tetracosenoic acid 24:1(n-11) of total fatty acids; Tetradecanoic acid of total fatty acids; Treatment: nitrate; Treatment: phosphate; Treatment: salinity; Treatment: silicate; Treatment: temperature; trophic markers; Type of study; Unialgal cultures cultivation; WoRMS |
| url | https://doi.org/10.1594/PANGAEA.987819 |