Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Dataset Open Access |
| Lenguaje: | en |
| Publicado: |
PANGAEA
2026
|
| Materias: | |
| Acceso en línea: | https://doi.org/10.1594/PANGAEA.987854 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| _version_ | 1867171902560141312 |
|---|---|
| author | Leu, Eva Šupraha, Luka Graeve, Martin Fahl, Kirsten Müller, Anja Müller, Juliane Lunden, Jonas Woll, Matthias Kohlbach, Doreen |
| author_facet | Leu, Eva Šupraha, Luka Graeve, Martin Fahl, Kirsten Müller, Anja Müller, Juliane Lunden, Jonas Woll, Matthias Kohlbach, Doreen |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | During the AO2023-1 expedition (1-21 June 2023), Melosira assemblages were collected using a handpump at a sampling site in Fram Strait. In addition to performing physiological measurements and filtering the material for trophic markers (fatty acids, stable isotope ratios of fatty acids, sterols and highly branched isoprenoids (HBIs), living material was brought back to Akvaplan-niva in Oslo and two strains were established as unialgal cultures. The cultures are maintained in artificial seawater-based L1 medium at 4°C and 25 µmol m-2 s-1 light with a 16:8 L:D cycle. Lipids were extracted from freeze-dried samples following the method of Folch et al. (1957), using a dichloromethane/methanol mixture (2:1, v/v) in a sonication bath. The extracted lipids were converted to fatty acid methyl esters (FAMEs) and analyzed by gas chromatography (Agilent 6890N Network GC, Agilent Technologies) equipped with a DB-FFAP capillary column (30 m length, 0.25 mm inner diameter, 0.25 μm film thickness), split injection, and a flame ionization detector. The temperature program ranged from 160°C to 240°C. Carbon stable isotope ratios δ13C were measured directly from FA extracts for selected biomarker FAs—14:0, 16:0, 16:1(n-7), and 20:5(n-3)—using a Thermo GC-c-IRMS system. This setup included a Trace GC Ultra gas chromatograph, a GC Isolink interface, and a Delta V Plus isotope ratio mass spectrometer, all connected via a Conflo IV interface (Thermo Scientific). FAMEs, dissolved in hexane, were injected in splitless mode and separated on a DB-FFAP capillary column (60 m length, 0.25 mm inner diameter, 0.25 μm film thickness). Samples were analysed in triplicates. Stable isotope analysis was conducted to complement datasets on fatty acid and sterol proportions. Collectively, the datasets enable a detailed characterization of the biochemical composition of different Melosira arctica-dominated algal assemblages from the Arctic Ocean, and a comparison with a unialgal culture of this important species. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_987854 |
| institution | PANGAEA |
| language | en |
| publishDate | 2026 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Carbon isotopic compositions of fatty acids in Melosira assemblages, and subsequently isolated unialgal cultures collected in June 2023 during the RV Kronprins Haakon cruise 2023-1 in Fram Strait Leu, Eva Šupraha, Luka Graeve, Martin Fahl, Kirsten Müller, Anja Müller, Juliane Lunden, Jonas Woll, Matthias Kohlbach, Doreen 2024007009; 9-Hexadecenoic acid of total fatty acids, δ13C; all-cis-5,8,11,14,17-Eicosapentaenoic acid, δ13C; AO2023-1; AO2023-1_MA1; Arctic microalgae; compound-specific stable isotopes; Date/time end, experiment; Date/time start, experiment; DEPTH, water; Depth comment; Event label; Experimental treatment; fatty acids; Fram Strait; Gas chromatograph-mass spectrometer; GC-MS; Genus, unique identification; Genus, unique identification (Semantic URI); Genus, unique identification (URI); Hand pump; Hexadecanoic acid, δ13C; HP; Kronprins Haakon; Melosira; Reference in WoRMS; Replicate; Sample code/label; Standard cultivation; Station label; Tetradecanoic acid, δ13C; Treatment: nitrate; Treatment: phosphate; Treatment: salinity; Treatment: silicate; Treatment: temperature; trophic markers; Type of study; Unialgal cultures cultivation; WoRMS During the AO2023-1 expedition (1-21 June 2023), Melosira assemblages were collected using a handpump at a sampling site in Fram Strait. In addition to performing physiological measurements and filtering the material for trophic markers (fatty acids, stable isotope ratios of fatty acids, sterols and highly branched isoprenoids (HBIs), living material was brought back to Akvaplan-niva in Oslo and two strains were established as unialgal cultures. The cultures are maintained in artificial seawater-based L1 medium at 4°C and 25 µmol m-2 s-1 light with a 16:8 L:D cycle. Lipids were extracted from freeze-dried samples following the method of Folch et al. (1957), using a dichloromethane/methanol mixture (2:1, v/v) in a sonication bath. The extracted lipids were converted to fatty acid methyl esters (FAMEs) and analyzed by gas chromatography (Agilent 6890N Network GC, Agilent Technologies) equipped with a DB-FFAP capillary column (30 m length, 0.25 mm inner diameter, 0.25 μm film thickness), split injection, and a flame ionization detector. The temperature program ranged from 160°C to 240°C. Carbon stable isotope ratios δ13C were measured directly from FA extracts for selected biomarker FAs—14:0, 16:0, 16:1(n-7), and 20:5(n-3)—using a Thermo GC-c-IRMS system. This setup included a Trace GC Ultra gas chromatograph, a GC Isolink interface, and a Delta V Plus isotope ratio mass spectrometer, all connected via a Conflo IV interface (Thermo Scientific). FAMEs, dissolved in hexane, were injected in splitless mode and separated on a DB-FFAP capillary column (60 m length, 0.25 mm inner diameter, 0.25 μm film thickness). Samples were analysed in triplicates. Stable isotope analysis was conducted to complement datasets on fatty acid and sterol proportions. Collectively, the datasets enable a detailed characterization of the biochemical composition of different Melosira arctica-dominated algal assemblages from the Arctic Ocean, and a comparison with a unialgal culture of this important species. |
| title | Carbon isotopic compositions of fatty acids in Melosira assemblages, and subsequently isolated unialgal cultures collected in June 2023 during the RV Kronprins Haakon cruise 2023-1 in Fram Strait |
| topic | 2024007009; 9-Hexadecenoic acid of total fatty acids, δ13C; all-cis-5,8,11,14,17-Eicosapentaenoic acid, δ13C; AO2023-1; AO2023-1_MA1; Arctic microalgae; compound-specific stable isotopes; Date/time end, experiment; Date/time start, experiment; DEPTH, water; Depth comment; Event label; Experimental treatment; fatty acids; Fram Strait; Gas chromatograph-mass spectrometer; GC-MS; Genus, unique identification; Genus, unique identification (Semantic URI); Genus, unique identification (URI); Hand pump; Hexadecanoic acid, δ13C; HP; Kronprins Haakon; Melosira; Reference in WoRMS; Replicate; Sample code/label; Standard cultivation; Station label; Tetradecanoic acid, δ13C; Treatment: nitrate; Treatment: phosphate; Treatment: salinity; Treatment: silicate; Treatment: temperature; trophic markers; Type of study; Unialgal cultures cultivation; WoRMS |
| url | https://doi.org/10.1594/PANGAEA.987854 |