| _version_ | 1867170141674930176 |
|---|---|
| author | Pastor, Ada Holmboe, Cecilie Marie Hartvig Han, Xingguo Frossard, Aline Romaní, Anna M Kjær, Jakob B Tank, Jennifer Giménez-Grau, Pau Hille, Erika D'Acqui, Luigi P Catalán, Núria Riis, Tenna |
| author_facet | Pastor, Ada Holmboe, Cecilie Marie Hartvig Han, Xingguo Frossard, Aline Romaní, Anna M Kjær, Jakob B Tank, Jennifer Giménez-Grau, Pau Hille, Erika D'Acqui, Luigi P Catalán, Núria Riis, Tenna |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | This study integrates molecular, environmental, and spatiotemporal data to examine the structure and function of streambed microbial communities across Arctic regions experiencing rapid climate change. Sediment samples were collected from 28 streams spanning four Arctic regions during summer 2021, including 15 Greenlandic streams that were sampled three times throughout the open-water season to capture temporal variation. Microbial community composition was characterized using 16S rRNA gene sequencing, while functional potential for nitrogen cycling was quantified through qPCR targeting six key genes involved in N-fixation (nifH), nitrification (amoA, nxrB), and denitrification (nirS, norB, nosZ). Environmental data included catchment characteristics, streamwater and sediment caractheristics. These molecular and environmental datasets were integrated with spatial and temporal sampling metadata to assess biogeographic and seasonal trends. Together, these data provide a comprehensive framework for understanding how Arctic stream microbial communities and nitrogen cycling functions respond to spatial heterogeneity and seasonal dynamics under ongoing climate change. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_988290 |
| institution | PANGAEA |
| language | en |
| publishDate | 2026 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Microbial Nitrogen Cycling Genes in Arctic Streams Pastor, Ada Holmboe, Cecilie Marie Hartvig Han, Xingguo Frossard, Aline Romaní, Anna M Kjær, Jakob B Tank, Jennifer Giménez-Grau, Pau Hille, Erika D'Acqui, Luigi P Catalán, Núria Riis, Tenna 16S rRNA gene, copy number, per unit sediment dry mass; Alaska North Slope; Ammonium; AOA-amoA gene, copy number, per unit sediment dry mass; AOB-amoA gene, copy number, per unit sediment dry mass; Arctic; Aucella_microbes; Austre-Bayelva_microbes; Bayelva_microbes; Carbon; Carbon, organic, dissolved; Caribou_Creek_microbes; Catchment area; Conductivity, electrical; DATE/TIME; diversity; Elevation of event; Event label; Grænseelv_microbes; Havipak_Creek_microbes; Imnavait_microbes; Inuvik region, Canada; Jurassic_1_microbes; Jurassic_2_microbes; Kærelv_A_microbes; Kærelv_C_microbes; Kuparuk_microbes; Latitude of event; Lindemann_tributary_microbes; Longitude of event; Loss on ignition; MULT; Multiple investigations; Mørebekken_microbes; nifH gene, copy number, per unit sediment dry mass; nirS gene, copy number, per unit sediment dry mass; Nitrate; Nitrogen; Nitrogen, organic, dissolved; Nitrogen, total dissolved; nitrogen genes; Normalized Difference Vegetation Index; Northeast Greenland; nosZ gene, copy number, per unit sediment dry mass; nrxB gene, copy number, per unit sediment dry mass; Oksrukuyik_microbes; Palnatoke_microbes; pH; qnorB gene, copy number, per unit sediment dry mass; Rengleng_River_microbes; Sample code/label; Sediment; Size fraction > 2 mm, gravel; Size fraction 0.200-0.063 mm, fine sand; Size fraction 0.630-0.200 mm, medium sand; Size fraction 2.000-0.630 mm, coarse sand; Slope; Storvatenet_microbes; stream; Svalbard; Temperature, water; Toolik_Inlet_microbes; Trevor_microbes; Tørvedammen_microbes; Voitelva_microbes; water chemistry; Watershed; West_1_microbes; West_2_microbes; West_3_microbes; West_4_microbes; West_5_microbes; Zackenberg_River_microbes This study integrates molecular, environmental, and spatiotemporal data to examine the structure and function of streambed microbial communities across Arctic regions experiencing rapid climate change. Sediment samples were collected from 28 streams spanning four Arctic regions during summer 2021, including 15 Greenlandic streams that were sampled three times throughout the open-water season to capture temporal variation. Microbial community composition was characterized using 16S rRNA gene sequencing, while functional potential for nitrogen cycling was quantified through qPCR targeting six key genes involved in N-fixation (nifH), nitrification (amoA, nxrB), and denitrification (nirS, norB, nosZ). Environmental data included catchment characteristics, streamwater and sediment caractheristics. These molecular and environmental datasets were integrated with spatial and temporal sampling metadata to assess biogeographic and seasonal trends. Together, these data provide a comprehensive framework for understanding how Arctic stream microbial communities and nitrogen cycling functions respond to spatial heterogeneity and seasonal dynamics under ongoing climate change. |
| title | Microbial Nitrogen Cycling Genes in Arctic Streams |
| topic | 16S rRNA gene, copy number, per unit sediment dry mass; Alaska North Slope; Ammonium; AOA-amoA gene, copy number, per unit sediment dry mass; AOB-amoA gene, copy number, per unit sediment dry mass; Arctic; Aucella_microbes; Austre-Bayelva_microbes; Bayelva_microbes; Carbon; Carbon, organic, dissolved; Caribou_Creek_microbes; Catchment area; Conductivity, electrical; DATE/TIME; diversity; Elevation of event; Event label; Grænseelv_microbes; Havipak_Creek_microbes; Imnavait_microbes; Inuvik region, Canada; Jurassic_1_microbes; Jurassic_2_microbes; Kærelv_A_microbes; Kærelv_C_microbes; Kuparuk_microbes; Latitude of event; Lindemann_tributary_microbes; Longitude of event; Loss on ignition; MULT; Multiple investigations; Mørebekken_microbes; nifH gene, copy number, per unit sediment dry mass; nirS gene, copy number, per unit sediment dry mass; Nitrate; Nitrogen; Nitrogen, organic, dissolved; Nitrogen, total dissolved; nitrogen genes; Normalized Difference Vegetation Index; Northeast Greenland; nosZ gene, copy number, per unit sediment dry mass; nrxB gene, copy number, per unit sediment dry mass; Oksrukuyik_microbes; Palnatoke_microbes; pH; qnorB gene, copy number, per unit sediment dry mass; Rengleng_River_microbes; Sample code/label; Sediment; Size fraction > 2 mm, gravel; Size fraction 0.200-0.063 mm, fine sand; Size fraction 0.630-0.200 mm, medium sand; Size fraction 2.000-0.630 mm, coarse sand; Slope; Storvatenet_microbes; stream; Svalbard; Temperature, water; Toolik_Inlet_microbes; Trevor_microbes; Tørvedammen_microbes; Voitelva_microbes; water chemistry; Watershed; West_1_microbes; West_2_microbes; West_3_microbes; West_4_microbes; West_5_microbes; Zackenberg_River_microbes |
| url | https://doi.org/10.1594/PANGAEA.988290 |