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  • Dissolved nitrous oxide (N₂O) concentrations were measured in the sea surface microlayer (SML, upper 1 mm) and the underlying water (ULW) during the BASS mesocosm study conducted with Jade Bay (southern North Sea) water from 16 May to 16 June 2023. The dataset contains N₂O concentrations from both water layers throughout the experimental period. Sampling of the SML was carried out using the glass plate method. The measurements cover conditions before, during and after a phytoplankton bloom.