Candidate genes involved in biosynthesis and degradation of the main extracellular matrix polysaccharides of brown algae and their probable evolutionary history.
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| Format: | Artículo científico |
| Language: | en |
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BMC genomics
2024
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| author | Mazéas, Lisa Bouguerba-Collin, Ahlem Cock, J Mark Denoeud, France Godfroy, Olivier Brillet-Guéguen, Loraine Barbeyron, Tristan Lipinska, Agnieszka P Delage, Ludovic Corre, Erwan Drula, Elodie Henrissat, Bernard Czjzek, Mirjam Terrapon, Nicolas Hervé, Cécile |
| author_facet | Mazéas, Lisa Bouguerba-Collin, Ahlem Cock, J Mark Denoeud, France Godfroy, Olivier Brillet-Guéguen, Loraine Barbeyron, Tristan Lipinska, Agnieszka P Delage, Ludovic Corre, Erwan Drula, Elodie Henrissat, Bernard Czjzek, Mirjam Terrapon, Nicolas Hervé, Cécile Mazéas, Lisa Bouguerba-Collin, Ahlem Cock, J Mark Denoeud, France Godfroy, Olivier Brillet-Guéguen, Loraine Barbeyron, Tristan Lipinska, Agnieszka P Delage, Ludovic Corre, Erwan Drula, Elodie Henrissat, Bernard Czjzek, Mirjam Terrapon, Nicolas Hervé, Cécile |
| collection | PubMed - marine biology |
| contents | Candidate genes involved in biosynthesis and degradation of the main extracellular matrix polysaccharides of brown algae and their probable evolutionary history. Mazéas, Lisa Bouguerba-Collin, Ahlem Cock, J Mark Denoeud, France Godfroy, Olivier Brillet-Guéguen, Loraine Barbeyron, Tristan Lipinska, Agnieszka P Delage, Ludovic Corre, Erwan Drula, Elodie Henrissat, Bernard Czjzek, Mirjam Terrapon, Nicolas Hervé, Cécile Phaeophyceae Phylogeny Polysaccharides Extracellular Matrix Evolution, Molecular Alginates Genomics Brown algae belong to the Stramenopiles phylum and are phylogenetically distant from plants and other multicellular organisms. This independent evolutionary history has shaped brown algae with numerous metabolic characteristics specific to this group, including the synthesis of peculiar polysaccharides contained in their extracellular matrix (ECM). Alginates and fucose-containing sulphated polysaccharides (FCSPs), the latter including fucans, are the main components of ECMs. However, the metabolic pathways of these polysaccharides remain poorly described due to a lack of genomic data. An extensive genomic dataset has been recently released for brown algae and their close sister species, for which we previously performed an expert annotation of key genes involved in ECM-carbohydrate metabolisms. Here we provide a deeper analysis of this set of genes using comparative genomics, phylogenetics analyses, and protein modelling. Two key gene families involved in both the synthesis and degradation of alginate were suggested to have been acquired by the common ancestor of brown algae and their closest sister species Schizocladia ischiensis. Our analysis indicates that this assumption can be extended to additional metabolic steps, and thus to the whole alginate metabolic pathway. The pathway for the biosynthesis of fucans still remains biochemically unresolved and we also investigate putative fucosyltransferase genes that may harbour a fucan synthase activity in brown algae. Our analysis is the first extensive survey of carbohydrate-related enzymes in brown algae, and provides a valuable resource for future research into the glycome and ECM of brown algae. The expansion of specific families related to alginate metabolism may have represented an important prerequisite for the evolution of developmental complexity in brown algae. Our analysis questions the possible occurrence of FCSPs outside brown algae, notably within their closest sister taxon and in other Stramenopiles such as diatoms. Filling this knowledge gap in the future will help determine the origin and evolutionary history of fucan synthesis in eukaryotes. |
| format | Artículo científico |
| id | pubmed_39390408 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | BMC genomics |
| record_format | pubmed |
| spellingShingle | Candidate genes involved in biosynthesis and degradation of the main extracellular matrix polysaccharides of brown algae and their probable evolutionary history. Mazéas, Lisa Bouguerba-Collin, Ahlem Cock, J Mark Denoeud, France Godfroy, Olivier Brillet-Guéguen, Loraine Barbeyron, Tristan Lipinska, Agnieszka P Delage, Ludovic Corre, Erwan Drula, Elodie Henrissat, Bernard Czjzek, Mirjam Terrapon, Nicolas Hervé, Cécile Phaeophyceae Phylogeny Polysaccharides Extracellular Matrix Evolution, Molecular Alginates Genomics Candidate genes involved in biosynthesis and degradation of the main extracellular matrix polysaccharides of brown algae and their probable evolutionary history. Mazéas, Lisa Bouguerba-Collin, Ahlem Cock, J Mark Denoeud, France Godfroy, Olivier Brillet-Guéguen, Loraine Barbeyron, Tristan Lipinska, Agnieszka P Delage, Ludovic Corre, Erwan Drula, Elodie Henrissat, Bernard Czjzek, Mirjam Terrapon, Nicolas Hervé, Cécile Phaeophyceae Phylogeny Polysaccharides Extracellular Matrix Evolution, Molecular Alginates Genomics Brown algae belong to the Stramenopiles phylum and are phylogenetically distant from plants and other multicellular organisms. This independent evolutionary history has shaped brown algae with numerous metabolic characteristics specific to this group, including the synthesis of peculiar polysaccharides contained in their extracellular matrix (ECM). Alginates and fucose-containing sulphated polysaccharides (FCSPs), the latter including fucans, are the main components of ECMs. However, the metabolic pathways of these polysaccharides remain poorly described due to a lack of genomic data. An extensive genomic dataset has been recently released for brown algae and their close sister species, for which we previously performed an expert annotation of key genes involved in ECM-carbohydrate metabolisms. Here we provide a deeper analysis of this set of genes using comparative genomics, phylogenetics analyses, and protein modelling. Two key gene families involved in both the synthesis and degradation of alginate were suggested to have been acquired by the common ancestor of brown algae and their closest sister species Schizocladia ischiensis. Our analysis indicates that this assumption can be extended to additional metabolic steps, and thus to the whole alginate metabolic pathway. The pathway for the biosynthesis of fucans still remains biochemically unresolved and we also investigate putative fucosyltransferase genes that may harbour a fucan synthase activity in brown algae. Our analysis is the first extensive survey of carbohydrate-related enzymes in brown algae, and provides a valuable resource for future research into the glycome and ECM of brown algae. The expansion of specific families related to alginate metabolism may have represented an important prerequisite for the evolution of developmental complexity in brown algae. Our analysis questions the possible occurrence of FCSPs outside brown algae, notably within their closest sister taxon and in other Stramenopiles such as diatoms. Filling this knowledge gap in the future will help determine the origin and evolutionary history of fucan synthesis in eukaryotes. |
| title | Candidate genes involved in biosynthesis and degradation of the main extracellular matrix polysaccharides of brown algae and their probable evolutionary history. |
| topic | Phaeophyceae Phylogeny Polysaccharides Extracellular Matrix Evolution, Molecular Alginates Genomics |
| url | https://pubmed.ncbi.nlm.nih.gov/39390408/ |