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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
International journal of biological macromolecules
2024
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| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/39401616/ |
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| _version_ | 1868266292629209089 |
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| author | Ma, Yuzhen Yu, Huahua Teng, Lichao Geng, Hao Li, Rongfeng Xing, Ronge Liu, Song Li, Pengcheng |
| author_facet | Ma, Yuzhen Yu, Huahua Teng, Lichao Geng, Hao Li, Rongfeng Xing, Ronge Liu, Song Li, Pengcheng Ma, Yuzhen Yu, Huahua Teng, Lichao Geng, Hao Li, Rongfeng Xing, Ronge Liu, Song Li, Pengcheng |
| collection | PubMed - marine biology |
| contents | NnM469, a novel recombinant jellyfish venom metalloproteinase from Nemopilema nomurai, disrupted the cell matrix. Ma, Yuzhen Yu, Huahua Teng, Lichao Geng, Hao Li, Rongfeng Xing, Ronge Liu, Song Li, Pengcheng Animals Cnidarian Venoms Recombinant Proteins Amino Acid Sequence Humans Scyphozoa Cloning, Molecular Metalloproteases Zinc HaCaT Cells Molecular cloning and functional characterization of Nemopilema nomurai venom metalloproteinases have provided deeper insights into the pathogenesis of jellyfish dermatitis. This study reports a new cDNA clone from N. nomurai tentacle venom (Transcript sequence: ID469) encoding 362 amino acid residues, belonged to astacin family and capable of disrupting the cell matrix. The N. nomurai metalloproteinase 469 (NnM469) comprises a signal peptide and propeptide, followed by metalloproteinase domain containing a zinc-binding motif, and two ShKT domains. Notably, NnM469 features a zinc-binding motif (HEXXH) at the active site, within an extended sequence of HEXXHXXGFXHE, which is unique to astacin. Immunocytochemistry revealed that NnM469 is located in the stab tube and envelope of jellyfish nematocysts. Western blot and LC-MS/MS analysis confirmed that the NnM469 protein was successfully expressed using the Pichia pastoris expression system. The recombinant NnM469 could degrade the cell matrix, resulting in the death of HaCaT cells with an IC of 26.34 μg/mL. Finally, I-TASSER-generated structure and function predictions indicated that conserved Asp, His, His, His, and Tyr serve as key amino acid residues for the Zn ion binding in the catalytic center. In summary, the study of the molecular characteristics and function of NnM469 presents an opportunity to develop therapeutic interventions for jellyfish venom-induced dermatitis. |
| format | Artículo científico |
| id | pubmed_39401616 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | International journal of biological macromolecules |
| record_format | pubmed |
| spellingShingle | NnM469, a novel recombinant jellyfish venom metalloproteinase from Nemopilema nomurai, disrupted the cell matrix. Ma, Yuzhen Yu, Huahua Teng, Lichao Geng, Hao Li, Rongfeng Xing, Ronge Liu, Song Li, Pengcheng Animals Cnidarian Venoms Recombinant Proteins Amino Acid Sequence Humans Scyphozoa Cloning, Molecular Metalloproteases Zinc HaCaT Cells NnM469, a novel recombinant jellyfish venom metalloproteinase from Nemopilema nomurai, disrupted the cell matrix. Ma, Yuzhen Yu, Huahua Teng, Lichao Geng, Hao Li, Rongfeng Xing, Ronge Liu, Song Li, Pengcheng Animals Cnidarian Venoms Recombinant Proteins Amino Acid Sequence Humans Scyphozoa Cloning, Molecular Metalloproteases Zinc HaCaT Cells Molecular cloning and functional characterization of Nemopilema nomurai venom metalloproteinases have provided deeper insights into the pathogenesis of jellyfish dermatitis. This study reports a new cDNA clone from N. nomurai tentacle venom (Transcript sequence: ID469) encoding 362 amino acid residues, belonged to astacin family and capable of disrupting the cell matrix. The N. nomurai metalloproteinase 469 (NnM469) comprises a signal peptide and propeptide, followed by metalloproteinase domain containing a zinc-binding motif, and two ShKT domains. Notably, NnM469 features a zinc-binding motif (HEXXH) at the active site, within an extended sequence of HEXXHXXGFXHE, which is unique to astacin. Immunocytochemistry revealed that NnM469 is located in the stab tube and envelope of jellyfish nematocysts. Western blot and LC-MS/MS analysis confirmed that the NnM469 protein was successfully expressed using the Pichia pastoris expression system. The recombinant NnM469 could degrade the cell matrix, resulting in the death of HaCaT cells with an IC of 26.34 μg/mL. Finally, I-TASSER-generated structure and function predictions indicated that conserved Asp, His, His, His, and Tyr serve as key amino acid residues for the Zn ion binding in the catalytic center. In summary, the study of the molecular characteristics and function of NnM469 presents an opportunity to develop therapeutic interventions for jellyfish venom-induced dermatitis. |
| title | NnM469, a novel recombinant jellyfish venom metalloproteinase from Nemopilema nomurai, disrupted the cell matrix. |
| topic | Animals Cnidarian Venoms Recombinant Proteins Amino Acid Sequence Humans Scyphozoa Cloning, Molecular Metalloproteases Zinc HaCaT Cells |
| url | https://pubmed.ncbi.nlm.nih.gov/39401616/ |