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Main Authors: Li, Baofeng, Qi, Xiao, Li, Meng, Hua, Peiyan, Fu, Yilin, Li, Longxiang, Wang, Jincheng, Gao, Jing, Zhang, Guangxin, Tong, Ti, Wang, Hongda
Format: Artículo científico
Language:en
Published: Talanta 2025
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Online Access:https://pubmed.ncbi.nlm.nih.gov/39406104/
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author Li, Baofeng
Qi, Xiao
Li, Meng
Hua, Peiyan
Fu, Yilin
Li, Longxiang
Wang, Jincheng
Gao, Jing
Zhang, Guangxin
Tong, Ti
Wang, Hongda
author_facet Li, Baofeng
Qi, Xiao
Li, Meng
Hua, Peiyan
Fu, Yilin
Li, Longxiang
Wang, Jincheng
Gao, Jing
Zhang, Guangxin
Tong, Ti
Wang, Hongda
Li, Baofeng
Qi, Xiao
Li, Meng
Hua, Peiyan
Fu, Yilin
Li, Longxiang
Wang, Jincheng
Gao, Jing
Zhang, Guangxin
Tong, Ti
Wang, Hongda
collection PubMed - marine biology
contents Super-resolution imaging reveals the role of DDR1 cluster in NSCLC proliferation. Li, Baofeng Qi, Xiao Li, Meng Hua, Peiyan Fu, Yilin Li, Longxiang Wang, Jincheng Gao, Jing Zhang, Guangxin Tong, Ti Wang, Hongda Humans Carcinoma, Non-Small-Cell Lung Discoidin Domain Receptor 1 Lung Neoplasms Cell Proliferation Cell Line, Tumor Signal Transduction STAT3 Transcription Factor Discoidin domain receptor 1 (DDR1), a transmembrane protein, is crucial in tumor development. Prior studies have demonstrated a significant correlation between protein cluster distribution on the cell membrane and tumor evolution. However, the precise spatial distribution characteristics of DDR1 on cell membranes and their impact on tumor development remain unclear. In this study, we conducted gene expression analysis to investigate DDR1 expression in non-small cell lung cancer (NSCLC) and its association with patient prognosis. We also employed direct stochastic optical reconstruction microscopy (dSTORM) imaging to examine DDR1's spatial distribution in NSCLC cells and tissues. Our findings indicate that DDR1 forms larger, tighter, and more abundant clusters in cancer cells and tissues compared to their normal counterparts. Notably, we observed that the enhanced aggregation of DDR1 clusters increased the likelihood of interaction with SRC, thereby activating the SRC-STAT3 signaling pathway in NSCLC cells and promoting cell proliferation. This study provides novel insights into the role of DDR1 aggregation in tumor proliferation, confirms DDR1 as a potential tumor marker, and serves as a valuable resource for future drug development.
format Artículo científico
id pubmed_39406104
institution PubMed
language en
publishDate 2025
publisher Talanta
record_format pubmed
spellingShingle Super-resolution imaging reveals the role of DDR1 cluster in NSCLC proliferation.
Li, Baofeng
Qi, Xiao
Li, Meng
Hua, Peiyan
Fu, Yilin
Li, Longxiang
Wang, Jincheng
Gao, Jing
Zhang, Guangxin
Tong, Ti
Wang, Hongda
Humans
Carcinoma, Non-Small-Cell Lung
Discoidin Domain Receptor 1
Lung Neoplasms
Cell Proliferation
Cell Line, Tumor
Signal Transduction
STAT3 Transcription Factor
Super-resolution imaging reveals the role of DDR1 cluster in NSCLC proliferation. Li, Baofeng Qi, Xiao Li, Meng Hua, Peiyan Fu, Yilin Li, Longxiang Wang, Jincheng Gao, Jing Zhang, Guangxin Tong, Ti Wang, Hongda Humans Carcinoma, Non-Small-Cell Lung Discoidin Domain Receptor 1 Lung Neoplasms Cell Proliferation Cell Line, Tumor Signal Transduction STAT3 Transcription Factor Discoidin domain receptor 1 (DDR1), a transmembrane protein, is crucial in tumor development. Prior studies have demonstrated a significant correlation between protein cluster distribution on the cell membrane and tumor evolution. However, the precise spatial distribution characteristics of DDR1 on cell membranes and their impact on tumor development remain unclear. In this study, we conducted gene expression analysis to investigate DDR1 expression in non-small cell lung cancer (NSCLC) and its association with patient prognosis. We also employed direct stochastic optical reconstruction microscopy (dSTORM) imaging to examine DDR1's spatial distribution in NSCLC cells and tissues. Our findings indicate that DDR1 forms larger, tighter, and more abundant clusters in cancer cells and tissues compared to their normal counterparts. Notably, we observed that the enhanced aggregation of DDR1 clusters increased the likelihood of interaction with SRC, thereby activating the SRC-STAT3 signaling pathway in NSCLC cells and promoting cell proliferation. This study provides novel insights into the role of DDR1 aggregation in tumor proliferation, confirms DDR1 as a potential tumor marker, and serves as a valuable resource for future drug development.
title Super-resolution imaging reveals the role of DDR1 cluster in NSCLC proliferation.
topic Humans
Carcinoma, Non-Small-Cell Lung
Discoidin Domain Receptor 1
Lung Neoplasms
Cell Proliferation
Cell Line, Tumor
Signal Transduction
STAT3 Transcription Factor
url https://pubmed.ncbi.nlm.nih.gov/39406104/