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| Main Authors: | , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Molecules (Basel, Switzerland)
2024
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39407575/ |
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Table of Contents:
- Optimization of Ultrasonic-Assisted Extraction, Characterization and Antioxidant and Immunoregulatory Activities of Polysaccharides. Wang, Na Qin, Jingyi Chen, Zishuo Wu, Jiayi Xiang, Wenzhou Spirulina Antioxidants Polysaccharides Immunologic Factors Animals Polysaccharides, Bacterial Ultrasonic Waves Molecular Weight Mice Ultrasonics Chemical Fractionation This study aimed to enhance the ultrasonic-assisted extraction (UAE) yield of seawater polysaccharides (APPs) and investigate its structural characteristics and bioactivities. The optimization of UAE achieved a maximum crude polysaccharides yield of 14.78%. The optimal extraction conditions were a liquid-solid ratio of 30.00 mL/g, extraction temperature of 81 °C, ultrasonic power at 92 W and extraction time at 30 min. After purification through cellulose DEAE-52 and Sephadex G-100 columns, two polysaccharide elutions (APP-1 and APP-2) were obtained. APP-2 had stronger antioxidant and immunoregulatory activities than APP-1, thus the characterization of APP-2 was conducted. APP-2 was an acidic polysaccharide consisting of rhamnose, glucose, mannose and glucuronic acid at a ratio of 1.00:24.21:7.63:1.53. It possessed a molecular weight of 72.48 kDa. Additionally, APP-2 had linear and irregular spherical particles and amorphous structures, which contained pyranoid polysaccharides with alpha/beta glycosidic bonds. These findings offered the foundation for APP-2 as an antioxidant and immunomodulator applied in the food, pharmaceutical and cosmetic industries.