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| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of clinical medicine
2024
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39408071/ |
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| _version_ | 1868266292617674753 |
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| author | Martín-Sierra, Carmen Martins, Ricardo Coucelo, Margarida Abrantes, Ana Margarida Caetano Oliveira, Rui Tralhão, José Guilherme Botelho, Maria Filomena Furtado, Emanuel Domingues, Maria Rosário Paiva, Artur Laranjeira, Paula |
| author_facet | Martín-Sierra, Carmen Martins, Ricardo Coucelo, Margarida Abrantes, Ana Margarida Caetano Oliveira, Rui Tralhão, José Guilherme Botelho, Maria Filomena Furtado, Emanuel Domingues, Maria Rosário Paiva, Artur Laranjeira, Paula Martín-Sierra, Carmen Martins, Ricardo Coucelo, Margarida Abrantes, Ana Margarida Caetano Oliveira, Rui Tralhão, José Guilherme Botelho, Maria Filomena Furtado, Emanuel Domingues, Maria Rosário Paiva, Artur Laranjeira, Paula |
| collection | PubMed - marine biology |
| contents | Tumor Resection in Hepatic Carcinomas Restores Circulating T Regulatory Cells. Martín-Sierra, Carmen Martins, Ricardo Coucelo, Margarida Abrantes, Ana Margarida Caetano Oliveira, Rui Tralhão, José Guilherme Botelho, Maria Filomena Furtado, Emanuel Domingues, Maria Rosário Paiva, Artur Laranjeira, Paula : Cholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC) represent major primary liver cancers, affecting one of the most vital organs in the human body. T regulatory (Treg) cells play an important role in liver cancers through the immunosuppression of antitumor immune responses. The current study focuses on the characterization of circulating natural killer (NK) cells and T cell subsets, including Treg cells, in CCA and HCC patients, before and after surgical tumor resection, in order to understand the effect of tumor resection on the homeostasis of peripheral blood NK cells and T cells. : Whole blood assays were performed to monitor immune alterations and the functional competence of circulating lymphocytes in a group of ten healthy individuals, eight CCA patients, and twenty HCC patients, before and one month after the surgical procedure, using flow cytometry, cell sorting, and qRT-PCR. : Before tumor resection, both HCC and CCA patients display increased percentages of CD8 Treg cells and decreased frequencies of circulating CD4 Treg cells. Notwithstanding, no functional impairment was detected on circulating CD4 Treg cells, neither in CCA nor in HCC patients. Interestingly, the frequency of peripheral CD4 Treg cells increased from 0.55% ± 0.49 and 0.71% ± 0.54 (in CCA and HCC, respectively) at T0 to 0.99% ± 0.91 and 1.17% ± 0.33 (in CCA and HCC, respectively) at T1, following tumor resection. : Our results suggest mechanisms of immune modulation induced by tumor resection. |
| format | Artículo científico |
| id | pubmed_39408071 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Journal of clinical medicine |
| record_format | pubmed |
| spellingShingle | Tumor Resection in Hepatic Carcinomas Restores Circulating T Regulatory Cells. Martín-Sierra, Carmen Martins, Ricardo Coucelo, Margarida Abrantes, Ana Margarida Caetano Oliveira, Rui Tralhão, José Guilherme Botelho, Maria Filomena Furtado, Emanuel Domingues, Maria Rosário Paiva, Artur Laranjeira, Paula Tumor Resection in Hepatic Carcinomas Restores Circulating T Regulatory Cells. Martín-Sierra, Carmen Martins, Ricardo Coucelo, Margarida Abrantes, Ana Margarida Caetano Oliveira, Rui Tralhão, José Guilherme Botelho, Maria Filomena Furtado, Emanuel Domingues, Maria Rosário Paiva, Artur Laranjeira, Paula : Cholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC) represent major primary liver cancers, affecting one of the most vital organs in the human body. T regulatory (Treg) cells play an important role in liver cancers through the immunosuppression of antitumor immune responses. The current study focuses on the characterization of circulating natural killer (NK) cells and T cell subsets, including Treg cells, in CCA and HCC patients, before and after surgical tumor resection, in order to understand the effect of tumor resection on the homeostasis of peripheral blood NK cells and T cells. : Whole blood assays were performed to monitor immune alterations and the functional competence of circulating lymphocytes in a group of ten healthy individuals, eight CCA patients, and twenty HCC patients, before and one month after the surgical procedure, using flow cytometry, cell sorting, and qRT-PCR. : Before tumor resection, both HCC and CCA patients display increased percentages of CD8 Treg cells and decreased frequencies of circulating CD4 Treg cells. Notwithstanding, no functional impairment was detected on circulating CD4 Treg cells, neither in CCA nor in HCC patients. Interestingly, the frequency of peripheral CD4 Treg cells increased from 0.55% ± 0.49 and 0.71% ± 0.54 (in CCA and HCC, respectively) at T0 to 0.99% ± 0.91 and 1.17% ± 0.33 (in CCA and HCC, respectively) at T1, following tumor resection. : Our results suggest mechanisms of immune modulation induced by tumor resection. |
| title | Tumor Resection in Hepatic Carcinomas Restores Circulating T Regulatory Cells. |
| url | https://pubmed.ncbi.nlm.nih.gov/39408071/ |