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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Science (New York, N.Y.)
2024
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39418385/ |
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| _version_ | 1868266290219581440 |
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| author | Lien, Yun-Wei Amendola, Davide Lee, Kang Soo Bartlau, Nina Xu, Jingwei Furusawa, Go Polz, Martin F Stocker, Roman Weiss, Gregor L Pilhofer, Martin |
| author_facet | Lien, Yun-Wei Amendola, Davide Lee, Kang Soo Bartlau, Nina Xu, Jingwei Furusawa, Go Polz, Martin F Stocker, Roman Weiss, Gregor L Pilhofer, Martin Lien, Yun-Wei Amendola, Davide Lee, Kang Soo Bartlau, Nina Xu, Jingwei Furusawa, Go Polz, Martin F Stocker, Roman Weiss, Gregor L Pilhofer, Martin |
| collection | PubMed - marine biology |
| contents | Mechanism of bacterial predation via ixotrophy. Lien, Yun-Wei Amendola, Davide Lee, Kang Soo Bartlau, Nina Xu, Jingwei Furusawa, Go Polz, Martin F Stocker, Roman Weiss, Gregor L Pilhofer, Martin Bacterial Adhesion Bacteroidetes Cryoelectron Microscopy Single-Cell Analysis Type VI Secretion Systems Vibrio Flagella Ixotrophy is a contact-dependent predatory strategy of filamentous bacteria in aquatic environments for which the molecular mechanism remains unknown. We show that predator-prey contact can be established by gliding motility or extracellular assemblages we call "grappling hooks." Cryo-electron microscopy identified the grappling hooks as heptamers of a type IX secretion system substrate. After close predator-prey contact is established, cryo-electron tomography and functional assays showed that puncturing by a type VI secretion system mediated killing. Single-cell analyses with stable isotope-labeled prey revealed that prey components are taken up by the attacker. Depending on nutrient availability, insertion sequence elements toggle the activity of ixotrophy. A marine metagenomic time series shows coupled dynamics of ixotrophic bacteria and prey. We found that the mechanism of ixotrophy involves multiple cellular machineries, is conserved, and may shape microbial populations in the environment. |
| format | Artículo científico |
| id | pubmed_39418385 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Science (New York, N.Y.) |
| record_format | pubmed |
| spellingShingle | Mechanism of bacterial predation via ixotrophy. Lien, Yun-Wei Amendola, Davide Lee, Kang Soo Bartlau, Nina Xu, Jingwei Furusawa, Go Polz, Martin F Stocker, Roman Weiss, Gregor L Pilhofer, Martin Bacterial Adhesion Bacteroidetes Cryoelectron Microscopy Single-Cell Analysis Type VI Secretion Systems Vibrio Flagella Mechanism of bacterial predation via ixotrophy. Lien, Yun-Wei Amendola, Davide Lee, Kang Soo Bartlau, Nina Xu, Jingwei Furusawa, Go Polz, Martin F Stocker, Roman Weiss, Gregor L Pilhofer, Martin Bacterial Adhesion Bacteroidetes Cryoelectron Microscopy Single-Cell Analysis Type VI Secretion Systems Vibrio Flagella Ixotrophy is a contact-dependent predatory strategy of filamentous bacteria in aquatic environments for which the molecular mechanism remains unknown. We show that predator-prey contact can be established by gliding motility or extracellular assemblages we call "grappling hooks." Cryo-electron microscopy identified the grappling hooks as heptamers of a type IX secretion system substrate. After close predator-prey contact is established, cryo-electron tomography and functional assays showed that puncturing by a type VI secretion system mediated killing. Single-cell analyses with stable isotope-labeled prey revealed that prey components are taken up by the attacker. Depending on nutrient availability, insertion sequence elements toggle the activity of ixotrophy. A marine metagenomic time series shows coupled dynamics of ixotrophic bacteria and prey. We found that the mechanism of ixotrophy involves multiple cellular machineries, is conserved, and may shape microbial populations in the environment. |
| title | Mechanism of bacterial predation via ixotrophy. |
| topic | Bacterial Adhesion Bacteroidetes Cryoelectron Microscopy Single-Cell Analysis Type VI Secretion Systems Vibrio Flagella |
| url | https://pubmed.ncbi.nlm.nih.gov/39418385/ |