Protocol for screening potential targets for the treatment of hepatocellular carcinoma based on H22 tumor-bearing mouse model.
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| Format: | Artículo científico |
| Sprache: | en |
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STAR protocols
2024
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| _version_ | 1868266290212241409 |
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| author | Gao, Xiaoxiang Qiu, Yinghui Liao, Wei Zhao, Chao |
| author_facet | Gao, Xiaoxiang Qiu, Yinghui Liao, Wei Zhao, Chao Gao, Xiaoxiang Qiu, Yinghui Liao, Wei Zhao, Chao |
| collection | PubMed - marine biology |
| contents | Protocol for screening potential targets for the treatment of hepatocellular carcinoma based on H22 tumor-bearing mouse model. Gao, Xiaoxiang Qiu, Yinghui Liao, Wei Zhao, Chao Animals Carcinoma, Hepatocellular Mice Liver Neoplasms MicroRNAs Fluorouracil Disease Models, Animal Cell Line, Tumor Regulating cancer-related microRNAs (miRNAs) may become a new generation of therapeutic modalities for cancer treatment. Here, we describe a protocol based on hepatoma-22 (H22) tumor-bearing mice to screen potential targets for treating hepatocellular carcinoma (HCC). We detail the construction of H22 tumor-bearing mice and treatment with two natural compounds, Ulva lactuca L. polysaccharide (ULP) and 5-fluorouracil (5FU). We further describe the isolation of the tumor tissues for miRNA sequencing and the discovery and validation of potential miRNA gene targets against HCC. For complete details on the use and execution of this protocol, please refer to Qiu et al.. |
| format | Artículo científico |
| id | pubmed_39423126 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | STAR protocols |
| record_format | pubmed |
| spellingShingle | Protocol for screening potential targets for the treatment of hepatocellular carcinoma based on H22 tumor-bearing mouse model. Gao, Xiaoxiang Qiu, Yinghui Liao, Wei Zhao, Chao Animals Carcinoma, Hepatocellular Mice Liver Neoplasms MicroRNAs Fluorouracil Disease Models, Animal Cell Line, Tumor Protocol for screening potential targets for the treatment of hepatocellular carcinoma based on H22 tumor-bearing mouse model. Gao, Xiaoxiang Qiu, Yinghui Liao, Wei Zhao, Chao Animals Carcinoma, Hepatocellular Mice Liver Neoplasms MicroRNAs Fluorouracil Disease Models, Animal Cell Line, Tumor Regulating cancer-related microRNAs (miRNAs) may become a new generation of therapeutic modalities for cancer treatment. Here, we describe a protocol based on hepatoma-22 (H22) tumor-bearing mice to screen potential targets for treating hepatocellular carcinoma (HCC). We detail the construction of H22 tumor-bearing mice and treatment with two natural compounds, Ulva lactuca L. polysaccharide (ULP) and 5-fluorouracil (5FU). We further describe the isolation of the tumor tissues for miRNA sequencing and the discovery and validation of potential miRNA gene targets against HCC. For complete details on the use and execution of this protocol, please refer to Qiu et al.. |
| title | Protocol for screening potential targets for the treatment of hepatocellular carcinoma based on H22 tumor-bearing mouse model. |
| topic | Animals Carcinoma, Hepatocellular Mice Liver Neoplasms MicroRNAs Fluorouracil Disease Models, Animal Cell Line, Tumor |
| url | https://pubmed.ncbi.nlm.nih.gov/39423126/ |