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| Format: | Artículo científico |
| Language: | en |
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Toxicon : official journal of the International Society on Toxinology
2024
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39426425/ |
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| author | M H Subari, Ily Azzedine Alaia Mutthumanickam, Gowri Hj Jikal, Muhammad Shah, Muhammad Dawood Tan, Jen Kit Tan, Yee Shin Palasubermaniam, Praneetha Palaniveloo, Kishneth Sathiya Seelan, Jaya Seelan |
| author_facet | M H Subari, Ily Azzedine Alaia Mutthumanickam, Gowri Hj Jikal, Muhammad Shah, Muhammad Dawood Tan, Jen Kit Tan, Yee Shin Palasubermaniam, Praneetha Palaniveloo, Kishneth Sathiya Seelan, Jaya Seelan M H Subari, Ily Azzedine Alaia Mutthumanickam, Gowri Hj Jikal, Muhammad Shah, Muhammad Dawood Tan, Jen Kit Tan, Yee Shin Palasubermaniam, Praneetha Palaniveloo, Kishneth Sathiya Seelan, Jaya Seelan |
| collection | PubMed - marine biology |
| contents | LC-MS/MS profiling of the poisonous wild mushroom, Entoloma mastoideum, (Entolomataceae, Basidiomycota) in Sabah (Northern Borneo), Malaysia. M H Subari, Ily Azzedine Alaia Mutthumanickam, Gowri Hj Jikal, Muhammad Shah, Muhammad Dawood Tan, Jen Kit Tan, Yee Shin Palasubermaniam, Praneetha Palaniveloo, Kishneth Sathiya Seelan, Jaya Seelan Mushroom Poisoning Tandem Mass Spectrometry Malaysia Phylogeny Agaricales Chromatography, Liquid Humans Mycotoxins Basidiomycota Borneo Liquid Chromatography-Mass Spectrometry In Malaysia, mushroom poisoning poses a significant public health concern, particularly as local communities frequently consume wild mushrooms without fully recognizing the potential dangers they present. Ingestion of wild mushrooms, which resemble edible species but contain deadly chemicals, is the main cause of poisoning risk. The genus Entoloma (Fr.) P. Kumm is dispersed worldwide, with over 1500 species recorded. Entoloma mastoideum, a poisonous mushroom, was recorded for the first time at Kota Marudu district, Sabah, Malaysia, where ten cases of mushroom poisoning outbreaks were recorded during the rainy season of October 2019. The morphological characters of the specimens were documented and their microscopic features were analyzed using compound microscope and scanning electron microscope. A phylogenetic tree was constructed using Maximum likelihood and Bayesian analysis. Chemical profiling of the poisonous mushroom specimen was done via liquid chromatograph mass spectrometry (LC-MS/MS) to identify toxic metabolites. The phylogenetic analysis showed that the Bornean E. mastoideum is closely related to the Chinese isolates (100% BS/1.0 PP). LC-MS/MS profiling detected a total of 162 metabolites that were classified into ten general groups, where several toxic compounds were detected amongst the aromatics, essential amino acids, and fatty acid derivatives. The toxic compounds identified in the mushroom extract, including amino acid derivatives such as 3,4,5,6-tetrahydroxyynorleucine, valpromide, and betaine, have been reported to cause neurotoxicity, cardiotoxicity, liver damage, and gastrointestinal harm. The presence of these toxic compounds underscores the need for caution when consuming wild mushrooms. Further research on poisonous Entoloma species is vital for developing accurate identification methods and understanding their toxic potential. This knowledge is essential for enhancing public awareness, preventing mushroom poisoning incidents and safeguarding public health. |
| format | Artículo científico |
| id | pubmed_39426425 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Toxicon : official journal of the International Society on Toxinology |
| record_format | pubmed |
| spellingShingle | LC-MS/MS profiling of the poisonous wild mushroom, Entoloma mastoideum, (Entolomataceae, Basidiomycota) in Sabah (Northern Borneo), Malaysia. M H Subari, Ily Azzedine Alaia Mutthumanickam, Gowri Hj Jikal, Muhammad Shah, Muhammad Dawood Tan, Jen Kit Tan, Yee Shin Palasubermaniam, Praneetha Palaniveloo, Kishneth Sathiya Seelan, Jaya Seelan Mushroom Poisoning Tandem Mass Spectrometry Malaysia Phylogeny Agaricales Chromatography, Liquid Humans Mycotoxins Basidiomycota Borneo Liquid Chromatography-Mass Spectrometry LC-MS/MS profiling of the poisonous wild mushroom, Entoloma mastoideum, (Entolomataceae, Basidiomycota) in Sabah (Northern Borneo), Malaysia. M H Subari, Ily Azzedine Alaia Mutthumanickam, Gowri Hj Jikal, Muhammad Shah, Muhammad Dawood Tan, Jen Kit Tan, Yee Shin Palasubermaniam, Praneetha Palaniveloo, Kishneth Sathiya Seelan, Jaya Seelan Mushroom Poisoning Tandem Mass Spectrometry Malaysia Phylogeny Agaricales Chromatography, Liquid Humans Mycotoxins Basidiomycota Borneo Liquid Chromatography-Mass Spectrometry In Malaysia, mushroom poisoning poses a significant public health concern, particularly as local communities frequently consume wild mushrooms without fully recognizing the potential dangers they present. Ingestion of wild mushrooms, which resemble edible species but contain deadly chemicals, is the main cause of poisoning risk. The genus Entoloma (Fr.) P. Kumm is dispersed worldwide, with over 1500 species recorded. Entoloma mastoideum, a poisonous mushroom, was recorded for the first time at Kota Marudu district, Sabah, Malaysia, where ten cases of mushroom poisoning outbreaks were recorded during the rainy season of October 2019. The morphological characters of the specimens were documented and their microscopic features were analyzed using compound microscope and scanning electron microscope. A phylogenetic tree was constructed using Maximum likelihood and Bayesian analysis. Chemical profiling of the poisonous mushroom specimen was done via liquid chromatograph mass spectrometry (LC-MS/MS) to identify toxic metabolites. The phylogenetic analysis showed that the Bornean E. mastoideum is closely related to the Chinese isolates (100% BS/1.0 PP). LC-MS/MS profiling detected a total of 162 metabolites that were classified into ten general groups, where several toxic compounds were detected amongst the aromatics, essential amino acids, and fatty acid derivatives. The toxic compounds identified in the mushroom extract, including amino acid derivatives such as 3,4,5,6-tetrahydroxyynorleucine, valpromide, and betaine, have been reported to cause neurotoxicity, cardiotoxicity, liver damage, and gastrointestinal harm. The presence of these toxic compounds underscores the need for caution when consuming wild mushrooms. Further research on poisonous Entoloma species is vital for developing accurate identification methods and understanding their toxic potential. This knowledge is essential for enhancing public awareness, preventing mushroom poisoning incidents and safeguarding public health. |
| title | LC-MS/MS profiling of the poisonous wild mushroom, Entoloma mastoideum, (Entolomataceae, Basidiomycota) in Sabah (Northern Borneo), Malaysia. |
| topic | Mushroom Poisoning Tandem Mass Spectrometry Malaysia Phylogeny Agaricales Chromatography, Liquid Humans Mycotoxins Basidiomycota Borneo Liquid Chromatography-Mass Spectrometry |
| url | https://pubmed.ncbi.nlm.nih.gov/39426425/ |