DUF99 family proteins are novel endonucleases that cleave deoxyuridine on DNA substrates.
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| Format: | Artículo científico |
| Langue: | en |
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The Journal of biological chemistry
2024
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| _version_ | 1868266290205949952 |
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| author | Li, Jinquan Xia, Runyue Huang, Wen-Cong Gu, Jiazheng Li, Meng |
| author_facet | Li, Jinquan Xia, Runyue Huang, Wen-Cong Gu, Jiazheng Li, Meng Li, Jinquan Xia, Runyue Huang, Wen-Cong Gu, Jiazheng Li, Meng |
| collection | PubMed - marine biology |
| contents | DUF99 family proteins are novel endonucleases that cleave deoxyuridine on DNA substrates. Li, Jinquan Xia, Runyue Huang, Wen-Cong Gu, Jiazheng Li, Meng Archaeal Proteins Phylogeny Haloferax volcanii Deoxyuridine Uracil-DNA Glycosidase Endonucleases Substrate Specificity DNA Repair DNA DNA deamination occurs constantly in a cell and causes DNA damage. As this damage can be deleterious, organisms have evolved many systems to eliminate it, such as Endonuclease V (Endo V). DUF99 family protein contains a domain of unknown function similar to Endo V but has not been experimentally characterized to date. Here, we show that DUF99 family proteins cleave the 3'-side of deoxyuridine (dU) on DNA substrates. Based on phylogenetic analysis, we designated this new protein family as Endonuclease dU (Endo_dU). We also observed that Endo_dU coding gene frequently colocalizes with that of uracil-DNA glycosylase (UDG) in halophilic archaea, and we further performed gene knockout of Endo_dU gene on Haloferax volcanii. The transcription level of UDG gene on Endo_dU knockout strain was increased when induced by sodium bisulfite. Thus, we hypothesize that Endo_dU establishes a new endonuclease family with broad phylogenetic distribution and may participate in DNA repair. |
| format | Artículo científico |
| id | pubmed_39426726 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | The Journal of biological chemistry |
| record_format | pubmed |
| spellingShingle | DUF99 family proteins are novel endonucleases that cleave deoxyuridine on DNA substrates. Li, Jinquan Xia, Runyue Huang, Wen-Cong Gu, Jiazheng Li, Meng Archaeal Proteins Phylogeny Haloferax volcanii Deoxyuridine Uracil-DNA Glycosidase Endonucleases Substrate Specificity DNA Repair DNA DUF99 family proteins are novel endonucleases that cleave deoxyuridine on DNA substrates. Li, Jinquan Xia, Runyue Huang, Wen-Cong Gu, Jiazheng Li, Meng Archaeal Proteins Phylogeny Haloferax volcanii Deoxyuridine Uracil-DNA Glycosidase Endonucleases Substrate Specificity DNA Repair DNA DNA deamination occurs constantly in a cell and causes DNA damage. As this damage can be deleterious, organisms have evolved many systems to eliminate it, such as Endonuclease V (Endo V). DUF99 family protein contains a domain of unknown function similar to Endo V but has not been experimentally characterized to date. Here, we show that DUF99 family proteins cleave the 3'-side of deoxyuridine (dU) on DNA substrates. Based on phylogenetic analysis, we designated this new protein family as Endonuclease dU (Endo_dU). We also observed that Endo_dU coding gene frequently colocalizes with that of uracil-DNA glycosylase (UDG) in halophilic archaea, and we further performed gene knockout of Endo_dU gene on Haloferax volcanii. The transcription level of UDG gene on Endo_dU knockout strain was increased when induced by sodium bisulfite. Thus, we hypothesize that Endo_dU establishes a new endonuclease family with broad phylogenetic distribution and may participate in DNA repair. |
| title | DUF99 family proteins are novel endonucleases that cleave deoxyuridine on DNA substrates. |
| topic | Archaeal Proteins Phylogeny Haloferax volcanii Deoxyuridine Uracil-DNA Glycosidase Endonucleases Substrate Specificity DNA Repair DNA |
| url | https://pubmed.ncbi.nlm.nih.gov/39426726/ |