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| Main Authors: | , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Cell reports
2024
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39427316/ |
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Table of Contents:
- A reverse transcriptase controls prophage genome reduction to promote phage dissemination in Pseudomonas aeruginosa biofilms. Guo, Yunxue Lin, Shituan Chen, Ran Gu, Jiayu Tang, Kaihao Nie, Zhaolong Huang, Zixian Weng, Juehua Lin, Jianzhong Liu, Tianlang Waldor, Matthew K Wang, Xiaoxue Pseudomonas aeruginosa Biofilms Prophages Genome, Viral RNA-Directed DNA Polymerase Pseudomonas Phages Bacteriophages Filamentous bacteriophages play a critical role in biofilm formation and virulence in the opportunistic pathogen Pseudomonas aeruginosa. Here, studies of the filamentous Pf4 prophage life cycle within P. aeruginosa biofilms revealed that the prophage-encoded reverse transcriptase (RT) regulates phage genome dynamics. The RT and the non-coding RNA PhrD collaborate to edit the Pf4 phage genome to generate superinfective Pf4 variants capable of rapid propagation within biofilms by preserving genes essential for virion assembly and reconstituting a promoter for the phage excisionase gene. Mutant cells emerge in biofilms where intact Pf4 prophages are replaced by these reduced-genome phage variants, further enhancing virion production. The discovery of RT's role in phage genome reduction expands understanding of RT functions and of the versatility of phage biology and its impact on microbial community dynamics within biofilms.