GTSF1 is required for transposon silencing in the unicellular eukaryote Paramecium tetraurelia.
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Nucleic acids research
2024
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| author | Wang, Chundi Lyv, Liping Solberg, Therese Zhang, Haoyue Wen, Zhiwei Gao, Feng |
| author_facet | Wang, Chundi Lyv, Liping Solberg, Therese Zhang, Haoyue Wen, Zhiwei Gao, Feng Wang, Chundi Lyv, Liping Solberg, Therese Zhang, Haoyue Wen, Zhiwei Gao, Feng |
| collection | PubMed - marine biology |
| contents | GTSF1 is required for transposon silencing in the unicellular eukaryote Paramecium tetraurelia. Wang, Chundi Lyv, Liping Solberg, Therese Zhang, Haoyue Wen, Zhiwei Gao, Feng Paramecium tetraurelia DNA Transposable Elements Gene Silencing Protozoan Proteins RNA, Small Interfering Argonaute Proteins Polycomb Repressive Complex 2 The PIWI-interacting RNA (piRNA) pathway is crucial for transposon repression and the maintenance of genomic integrity. Gametocyte-specific factor 1 (GTSF1), a PIWI-associated protein indispensable for transposon repression, has been recently shown to potentiate the catalytic activity of PIWI in many metazoans. Whether the requirement of GTSF1 extends to PIWI proteins beyond metazoans is unknown. In this study, we identified a homolog of GTSF1 in the unicellular eukaryote Paramecium tetraurelia (PtGtsf1) and found that its role as a PIWI-cofactor is conserved. PtGtsf1 interacts with PIWI (Ptiwi09) and Polycomb Repressive Complex 2 and is essential for PIWI-dependent DNA elimination of transposons during sexual development. PtGtsf1 is crucial for the degradation of PIWI-bound small RNAs that recognize the organism's own genomic sequences. Without PtGtsf1, self-matching small RNAs are not degraded and results in an accumulation of H3K9me3 and H3K27me3, which may disturb transposon recognition. Our results demonstrate that the PIWI-GTSF1 interaction also exists in unicellular eukaryotes with a role in transposon silencing. |
| format | Artículo científico |
| id | pubmed_39441077 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Nucleic acids research |
| record_format | pubmed |
| spellingShingle | GTSF1 is required for transposon silencing in the unicellular eukaryote Paramecium tetraurelia. Wang, Chundi Lyv, Liping Solberg, Therese Zhang, Haoyue Wen, Zhiwei Gao, Feng Paramecium tetraurelia DNA Transposable Elements Gene Silencing Protozoan Proteins RNA, Small Interfering Argonaute Proteins Polycomb Repressive Complex 2 GTSF1 is required for transposon silencing in the unicellular eukaryote Paramecium tetraurelia. Wang, Chundi Lyv, Liping Solberg, Therese Zhang, Haoyue Wen, Zhiwei Gao, Feng Paramecium tetraurelia DNA Transposable Elements Gene Silencing Protozoan Proteins RNA, Small Interfering Argonaute Proteins Polycomb Repressive Complex 2 The PIWI-interacting RNA (piRNA) pathway is crucial for transposon repression and the maintenance of genomic integrity. Gametocyte-specific factor 1 (GTSF1), a PIWI-associated protein indispensable for transposon repression, has been recently shown to potentiate the catalytic activity of PIWI in many metazoans. Whether the requirement of GTSF1 extends to PIWI proteins beyond metazoans is unknown. In this study, we identified a homolog of GTSF1 in the unicellular eukaryote Paramecium tetraurelia (PtGtsf1) and found that its role as a PIWI-cofactor is conserved. PtGtsf1 interacts with PIWI (Ptiwi09) and Polycomb Repressive Complex 2 and is essential for PIWI-dependent DNA elimination of transposons during sexual development. PtGtsf1 is crucial for the degradation of PIWI-bound small RNAs that recognize the organism's own genomic sequences. Without PtGtsf1, self-matching small RNAs are not degraded and results in an accumulation of H3K9me3 and H3K27me3, which may disturb transposon recognition. Our results demonstrate that the PIWI-GTSF1 interaction also exists in unicellular eukaryotes with a role in transposon silencing. |
| title | GTSF1 is required for transposon silencing in the unicellular eukaryote Paramecium tetraurelia. |
| topic | Paramecium tetraurelia DNA Transposable Elements Gene Silencing Protozoan Proteins RNA, Small Interfering Argonaute Proteins Polycomb Repressive Complex 2 |
| url | https://pubmed.ncbi.nlm.nih.gov/39441077/ |