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| Autores principales: | , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Journal of photochemistry and photobiology. B, Biology
2024
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| Materias: | |
| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/39442447/ |
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| _version_ | 1868266290183929856 |
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| author | Gómez-Villegas, Patricia Pérez-Rodríguez, Miguel Porres, Jesús M Prados, José C Melguizo, Consolación Vigara, Javier Moreno-Garrido, Ignacio León, Rosa |
| author_facet | Gómez-Villegas, Patricia Pérez-Rodríguez, Miguel Porres, Jesús M Prados, José C Melguizo, Consolación Vigara, Javier Moreno-Garrido, Ignacio León, Rosa Gómez-Villegas, Patricia Pérez-Rodríguez, Miguel Porres, Jesús M Prados, José C Melguizo, Consolación Vigara, Javier Moreno-Garrido, Ignacio León, Rosa |
| collection | PubMed - marine biology |
| contents | Metataxonomy and pigments analyses unravel microbial diversity and the relevance of retinal-based photoheterotrophy at different salinities in the Odiel Salterns (SW, Spain). Gómez-Villegas, Patricia Pérez-Rodríguez, Miguel Porres, Jesús M Prados, José C Melguizo, Consolación Vigara, Javier Moreno-Garrido, Ignacio León, Rosa Salinity RNA, Ribosomal, 16S Spain Photosynthesis RNA, Ribosomal, 18S Chlorophyll A Seawater Bacteria Retinaldehyde Phylogeny Chlorophyll Biodiversity Salinity has a strong influence on microorganisms distribution patterns and consequently on the relevance of photoheterotrophic metabolism, which since the discovery of proteorhodopsins is considered the main contributor to solar energy capture on the surface of the oceans. Solar salterns constitute an exceptional system for the simultaneous study of several salt concentrations, ranging from seawater, the most abundant environment on Earth, to saturated brine, one of the most extreme, which has been scarcely studied. In this study, pigment composition across the salinity gradient has been analyzed by spectrophotometry and RP-HPLC, and the influence of salinity on microbial diversity of the three domains of life has been evaluated by a metataxonomic study targeting hypervariable regions of 16S and 18S rRNA genes. Furthermore, based on the chlorophyll a and retinal content, we have estimated the relative abundance of rhodopsins and photosynthetic reaction centers, concluding that there is a strong correlation between the retinal/chlorophyll a ratio and salinity. Retinal-based photoheterotrophy is particularly important for prokaryotic survival in hypersaline environments, surpassing the sunlight energy captured by photosynthesis, and being more relevant as salinity increases. This fact has implications for understanding the survival of microorganisms in extreme conditions and the energy dynamics in solar salter ponds. |
| format | Artículo científico |
| id | pubmed_39442447 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Journal of photochemistry and photobiology. B, Biology |
| record_format | pubmed |
| spellingShingle | Metataxonomy and pigments analyses unravel microbial diversity and the relevance of retinal-based photoheterotrophy at different salinities in the Odiel Salterns (SW, Spain). Gómez-Villegas, Patricia Pérez-Rodríguez, Miguel Porres, Jesús M Prados, José C Melguizo, Consolación Vigara, Javier Moreno-Garrido, Ignacio León, Rosa Salinity RNA, Ribosomal, 16S Spain Photosynthesis RNA, Ribosomal, 18S Chlorophyll A Seawater Bacteria Retinaldehyde Phylogeny Chlorophyll Biodiversity Metataxonomy and pigments analyses unravel microbial diversity and the relevance of retinal-based photoheterotrophy at different salinities in the Odiel Salterns (SW, Spain). Gómez-Villegas, Patricia Pérez-Rodríguez, Miguel Porres, Jesús M Prados, José C Melguizo, Consolación Vigara, Javier Moreno-Garrido, Ignacio León, Rosa Salinity RNA, Ribosomal, 16S Spain Photosynthesis RNA, Ribosomal, 18S Chlorophyll A Seawater Bacteria Retinaldehyde Phylogeny Chlorophyll Biodiversity Salinity has a strong influence on microorganisms distribution patterns and consequently on the relevance of photoheterotrophic metabolism, which since the discovery of proteorhodopsins is considered the main contributor to solar energy capture on the surface of the oceans. Solar salterns constitute an exceptional system for the simultaneous study of several salt concentrations, ranging from seawater, the most abundant environment on Earth, to saturated brine, one of the most extreme, which has been scarcely studied. In this study, pigment composition across the salinity gradient has been analyzed by spectrophotometry and RP-HPLC, and the influence of salinity on microbial diversity of the three domains of life has been evaluated by a metataxonomic study targeting hypervariable regions of 16S and 18S rRNA genes. Furthermore, based on the chlorophyll a and retinal content, we have estimated the relative abundance of rhodopsins and photosynthetic reaction centers, concluding that there is a strong correlation between the retinal/chlorophyll a ratio and salinity. Retinal-based photoheterotrophy is particularly important for prokaryotic survival in hypersaline environments, surpassing the sunlight energy captured by photosynthesis, and being more relevant as salinity increases. This fact has implications for understanding the survival of microorganisms in extreme conditions and the energy dynamics in solar salter ponds. |
| title | Metataxonomy and pigments analyses unravel microbial diversity and the relevance of retinal-based photoheterotrophy at different salinities in the Odiel Salterns (SW, Spain). |
| topic | Salinity RNA, Ribosomal, 16S Spain Photosynthesis RNA, Ribosomal, 18S Chlorophyll A Seawater Bacteria Retinaldehyde Phylogeny Chlorophyll Biodiversity |
| url | https://pubmed.ncbi.nlm.nih.gov/39442447/ |