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| Main Authors: | , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Cell stem cell
2024
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39442525/ |
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| _version_ | 1868266290182881281 |
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| author | Zhu, Jialiang Zhong, Xinxing He, Huanjing Cao, Jingxiao Zhou, Zhengyang Dong, Jiebin Li, Honggang Zhang, Anqi Lyu, Yulin Li, Cheng Guan, Jingyang Deng, Hongkui |
| author_facet | Zhu, Jialiang Zhong, Xinxing He, Huanjing Cao, Jingxiao Zhou, Zhengyang Dong, Jiebin Li, Honggang Zhang, Anqi Lyu, Yulin Li, Cheng Guan, Jingyang Deng, Hongkui Zhu, Jialiang Zhong, Xinxing He, Huanjing Cao, Jingxiao Zhou, Zhengyang Dong, Jiebin Li, Honggang Zhang, Anqi Lyu, Yulin Li, Cheng Guan, Jingyang Deng, Hongkui |
| collection | PubMed - marine biology |
| contents | Generation of human expandable limb-bud-like progenitors via chemically induced dedifferentiation. Zhu, Jialiang Zhong, Xinxing He, Huanjing Cao, Jingxiao Zhou, Zhengyang Dong, Jiebin Li, Honggang Zhang, Anqi Lyu, Yulin Li, Cheng Guan, Jingyang Deng, Hongkui Humans Cell Dedifferentiation Limb Buds Cell Differentiation Stem Cells Cells, Cultured Cell Proliferation In certain highly regenerative animals, cellular dedifferentiation occurs after injury, allowing specialized cells to become progenitor cells for regeneration. However, this capacity is restricted in human cells due to reduced plasticity. Here, we introduce a chemical-induced dedifferentiation approach that reverts the differentiated cells to a progenitor-like state, conferring the features of human limb bud cells from human adult somatic cells. These chemically induced human limb-bud-like progenitors (hCiLBP cells) show a high degree of transcriptomic similarity to human embryonic limb bud progenitors. Importantly, we established culture conditions that allow hCiLBP cells to undergo extensive expansion while maintaining population homogeneity and long-term self-renewal capacity. Moreover, hCiLBP cells exhibit increased osteochondrogenic differentiation ability, providing an innovative platform for generation of skeletal lineage cell types. These results highlight a potential therapeutic approach for repairing damaged human tissues through reversal of developmental pathways from mature cells to expandable progenitor cells. |
| format | Artículo científico |
| id | pubmed_39442525 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Cell stem cell |
| record_format | pubmed |
| spellingShingle | Generation of human expandable limb-bud-like progenitors via chemically induced dedifferentiation. Zhu, Jialiang Zhong, Xinxing He, Huanjing Cao, Jingxiao Zhou, Zhengyang Dong, Jiebin Li, Honggang Zhang, Anqi Lyu, Yulin Li, Cheng Guan, Jingyang Deng, Hongkui Humans Cell Dedifferentiation Limb Buds Cell Differentiation Stem Cells Cells, Cultured Cell Proliferation Generation of human expandable limb-bud-like progenitors via chemically induced dedifferentiation. Zhu, Jialiang Zhong, Xinxing He, Huanjing Cao, Jingxiao Zhou, Zhengyang Dong, Jiebin Li, Honggang Zhang, Anqi Lyu, Yulin Li, Cheng Guan, Jingyang Deng, Hongkui Humans Cell Dedifferentiation Limb Buds Cell Differentiation Stem Cells Cells, Cultured Cell Proliferation In certain highly regenerative animals, cellular dedifferentiation occurs after injury, allowing specialized cells to become progenitor cells for regeneration. However, this capacity is restricted in human cells due to reduced plasticity. Here, we introduce a chemical-induced dedifferentiation approach that reverts the differentiated cells to a progenitor-like state, conferring the features of human limb bud cells from human adult somatic cells. These chemically induced human limb-bud-like progenitors (hCiLBP cells) show a high degree of transcriptomic similarity to human embryonic limb bud progenitors. Importantly, we established culture conditions that allow hCiLBP cells to undergo extensive expansion while maintaining population homogeneity and long-term self-renewal capacity. Moreover, hCiLBP cells exhibit increased osteochondrogenic differentiation ability, providing an innovative platform for generation of skeletal lineage cell types. These results highlight a potential therapeutic approach for repairing damaged human tissues through reversal of developmental pathways from mature cells to expandable progenitor cells. |
| title | Generation of human expandable limb-bud-like progenitors via chemically induced dedifferentiation. |
| topic | Humans Cell Dedifferentiation Limb Buds Cell Differentiation Stem Cells Cells, Cultured Cell Proliferation |
| url | https://pubmed.ncbi.nlm.nih.gov/39442525/ |