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| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Molecular ecology
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39450613/ |
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| _version_ | 1868266287751233536 |
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| author | Parsons, Kim M May, Samuel A Gold, Zachary Dahlheim, Marilyn Gabriele, Christine Straley, Janice M Moran, John R Goetz, Kimberly Zerbini, Alexandre N Park, Linda Morin, Phillip A |
| author_facet | Parsons, Kim M May, Samuel A Gold, Zachary Dahlheim, Marilyn Gabriele, Christine Straley, Janice M Moran, John R Goetz, Kimberly Zerbini, Alexandre N Park, Linda Morin, Phillip A Parsons, Kim M May, Samuel A Gold, Zachary Dahlheim, Marilyn Gabriele, Christine Straley, Janice M Moran, John R Goetz, Kimberly Zerbini, Alexandre N Park, Linda Morin, Phillip A |
| collection | PubMed - marine biology |
| contents | Using eDNA to Supplement Population Genetic Analyses for Cryptic Marine Species: Identifying Population Boundaries for Alaska Harbour Porpoises. Parsons, Kim M May, Samuel A Gold, Zachary Dahlheim, Marilyn Gabriele, Christine Straley, Janice M Moran, John R Goetz, Kimberly Zerbini, Alexandre N Park, Linda Morin, Phillip A Animals DNA, Mitochondrial Alaska Genetics, Population Polymorphism, Single Nucleotide Gene Flow Phocoena DNA, Environmental Sequence Analysis, DNA Genetic Variation Male Genotype Isolation by distance and biogeographical boundaries define patterns of population genetic structure for harbour porpoise along the Pacific coast from California to British Columbia. Until recently, inadequate sample sizes in many regions constrained efforts to characterise population genetic structure throughout the coastal waters of Alaska. Here, tissue samples from beachcast strandings and fisheries bycatch were supplemented with targeted environmental DNA (eDNA) samples in key regions of Alaska coastal and inland waters. Using a geographically explicit, hierarchical approach, we examined the genetic structure of Alaska harbour porpoises, using both mitochondrial DNA (mtDNA) sequence data and multilocus SNP genotypes. Despite a lack of evidence of genetic differentiation from nuclear SNP loci, patterns of relatedness and genetic differentiation from mtDNA suggest natal philopatry at multiple geographic scales, with limited gene flow among sites possibly mediated by male dispersal. A priori clustering of sampled areas at an intermediate scale (eastern and western Bering Sea, Gulf of Alaska and Southeast Alaska) best explained the genetic variance (12.37%) among regions. In addition, mtDNA differentiation between the Gulf of Alaska and eastern Bering Sea, and among regions within the Gulf of Alaska, indicated significant genetic structuring of harbour porpoise populations in Southeast Alaska. The targeted collection of eDNA samples from strata within Southeast Alaska was key for elevating the statistical power of our mtDNA dataset, and findings indicate limited dispersal between neighbouring strata within coastal and inland waters. These results provide evidence supporting a population boundary within the currently recognised Southeast Alaska Stock. Together, these findings will prove useful for ongoing management efforts to reduce fisheries conflict and conserve genetic diversity in this iconic coastal species. |
| format | Artículo científico |
| id | pubmed_39450613 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Molecular ecology |
| record_format | pubmed |
| spellingShingle | Using eDNA to Supplement Population Genetic Analyses for Cryptic Marine Species: Identifying Population Boundaries for Alaska Harbour Porpoises. Parsons, Kim M May, Samuel A Gold, Zachary Dahlheim, Marilyn Gabriele, Christine Straley, Janice M Moran, John R Goetz, Kimberly Zerbini, Alexandre N Park, Linda Morin, Phillip A Animals DNA, Mitochondrial Alaska Genetics, Population Polymorphism, Single Nucleotide Gene Flow Phocoena DNA, Environmental Sequence Analysis, DNA Genetic Variation Male Genotype Using eDNA to Supplement Population Genetic Analyses for Cryptic Marine Species: Identifying Population Boundaries for Alaska Harbour Porpoises. Parsons, Kim M May, Samuel A Gold, Zachary Dahlheim, Marilyn Gabriele, Christine Straley, Janice M Moran, John R Goetz, Kimberly Zerbini, Alexandre N Park, Linda Morin, Phillip A Animals DNA, Mitochondrial Alaska Genetics, Population Polymorphism, Single Nucleotide Gene Flow Phocoena DNA, Environmental Sequence Analysis, DNA Genetic Variation Male Genotype Isolation by distance and biogeographical boundaries define patterns of population genetic structure for harbour porpoise along the Pacific coast from California to British Columbia. Until recently, inadequate sample sizes in many regions constrained efforts to characterise population genetic structure throughout the coastal waters of Alaska. Here, tissue samples from beachcast strandings and fisheries bycatch were supplemented with targeted environmental DNA (eDNA) samples in key regions of Alaska coastal and inland waters. Using a geographically explicit, hierarchical approach, we examined the genetic structure of Alaska harbour porpoises, using both mitochondrial DNA (mtDNA) sequence data and multilocus SNP genotypes. Despite a lack of evidence of genetic differentiation from nuclear SNP loci, patterns of relatedness and genetic differentiation from mtDNA suggest natal philopatry at multiple geographic scales, with limited gene flow among sites possibly mediated by male dispersal. A priori clustering of sampled areas at an intermediate scale (eastern and western Bering Sea, Gulf of Alaska and Southeast Alaska) best explained the genetic variance (12.37%) among regions. In addition, mtDNA differentiation between the Gulf of Alaska and eastern Bering Sea, and among regions within the Gulf of Alaska, indicated significant genetic structuring of harbour porpoise populations in Southeast Alaska. The targeted collection of eDNA samples from strata within Southeast Alaska was key for elevating the statistical power of our mtDNA dataset, and findings indicate limited dispersal between neighbouring strata within coastal and inland waters. These results provide evidence supporting a population boundary within the currently recognised Southeast Alaska Stock. Together, these findings will prove useful for ongoing management efforts to reduce fisheries conflict and conserve genetic diversity in this iconic coastal species. |
| title | Using eDNA to Supplement Population Genetic Analyses for Cryptic Marine Species: Identifying Population Boundaries for Alaska Harbour Porpoises. |
| topic | Animals DNA, Mitochondrial Alaska Genetics, Population Polymorphism, Single Nucleotide Gene Flow Phocoena DNA, Environmental Sequence Analysis, DNA Genetic Variation Male Genotype |
| url | https://pubmed.ncbi.nlm.nih.gov/39450613/ |