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| Main Authors: | , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Marine drugs
2024
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39452847/ |
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| _version_ | 1868266287747039232 |
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| author | Zhang, Huiwen Sun, Chen Xia, Qing Li, Peihai Liu, Kechun Zhang, Yun |
| author_facet | Zhang, Huiwen Sun, Chen Xia, Qing Li, Peihai Liu, Kechun Zhang, Yun Zhang, Huiwen Sun, Chen Xia, Qing Li, Peihai Liu, Kechun Zhang, Yun |
| collection | PubMed - marine biology |
| contents | Brevianamide F Exerts Antithrombotic Effects by Modulating the MAPK Signaling Pathway and Coagulation Cascade. Zhang, Huiwen Sun, Chen Xia, Qing Li, Peihai Liu, Kechun Zhang, Yun Animals Zebrafish Molecular Docking Simulation Thrombosis Fibrinolytic Agents MAP Kinase Signaling System Blood Coagulation Disease Models, Animal Platelet Aggregation Existing antithrombotic drugs have side effects such as bleeding, and there is an urgent need to discover antithrombotic drugs with better efficacy and fewer side effects. In this study, a zebrafish thrombosis model was used to evaluate the antithrombotic activity and mechanism of Brevianamide F, a deep-sea natural product, with transcriptome sequencing analysis, RT-qPCR analysis, and molecular docking. The results revealed that Brevianamide F significantly attenuated the degree of platelet aggregation in the thrombus model zebrafish, leading to an increase in the number of circulating platelets, an augmentation in the return of blood to the heart, an elevated heart rate, and a significant restoration of caudal blood flow velocity. Transcriptome sequencing and RT-qPCR validation revealed that Brevianamide F may exert antithrombotic effects through the modulation of the MAPK signaling pathway and the coagulation cascade reaction. Molecular docking analysis further confirmed this result. This study provides a reference for the development of therapeutic drugs for thrombosis. |
| format | Artículo científico |
| id | pubmed_39452847 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Marine drugs |
| record_format | pubmed |
| spellingShingle | Brevianamide F Exerts Antithrombotic Effects by Modulating the MAPK Signaling Pathway and Coagulation Cascade. Zhang, Huiwen Sun, Chen Xia, Qing Li, Peihai Liu, Kechun Zhang, Yun Animals Zebrafish Molecular Docking Simulation Thrombosis Fibrinolytic Agents MAP Kinase Signaling System Blood Coagulation Disease Models, Animal Platelet Aggregation Brevianamide F Exerts Antithrombotic Effects by Modulating the MAPK Signaling Pathway and Coagulation Cascade. Zhang, Huiwen Sun, Chen Xia, Qing Li, Peihai Liu, Kechun Zhang, Yun Animals Zebrafish Molecular Docking Simulation Thrombosis Fibrinolytic Agents MAP Kinase Signaling System Blood Coagulation Disease Models, Animal Platelet Aggregation Existing antithrombotic drugs have side effects such as bleeding, and there is an urgent need to discover antithrombotic drugs with better efficacy and fewer side effects. In this study, a zebrafish thrombosis model was used to evaluate the antithrombotic activity and mechanism of Brevianamide F, a deep-sea natural product, with transcriptome sequencing analysis, RT-qPCR analysis, and molecular docking. The results revealed that Brevianamide F significantly attenuated the degree of platelet aggregation in the thrombus model zebrafish, leading to an increase in the number of circulating platelets, an augmentation in the return of blood to the heart, an elevated heart rate, and a significant restoration of caudal blood flow velocity. Transcriptome sequencing and RT-qPCR validation revealed that Brevianamide F may exert antithrombotic effects through the modulation of the MAPK signaling pathway and the coagulation cascade reaction. Molecular docking analysis further confirmed this result. This study provides a reference for the development of therapeutic drugs for thrombosis. |
| title | Brevianamide F Exerts Antithrombotic Effects by Modulating the MAPK Signaling Pathway and Coagulation Cascade. |
| topic | Animals Zebrafish Molecular Docking Simulation Thrombosis Fibrinolytic Agents MAP Kinase Signaling System Blood Coagulation Disease Models, Animal Platelet Aggregation |
| url | https://pubmed.ncbi.nlm.nih.gov/39452847/ |