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| Natura: | Artículo científico |
| Lingua: | en |
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International journal of systematic and evolutionary microbiology
2024
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| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/39453699/ |
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| author | Kim, Bora Jun, Min Ok Yang, Ah-In Joe, Sung-Hong Joe, Hae-In Sung, Hojun Kim, Ki Hyun Kim, Hyun Jung Shin, Na-Ri |
| author_facet | Kim, Bora Jun, Min Ok Yang, Ah-In Joe, Sung-Hong Joe, Hae-In Sung, Hojun Kim, Ki Hyun Kim, Hyun Jung Shin, Na-Ri Kim, Bora Jun, Min Ok Yang, Ah-In Joe, Sung-Hong Joe, Hae-In Sung, Hojun Kim, Ki Hyun Kim, Hyun Jung Shin, Na-Ri |
| collection | PubMed - marine biology |
| contents | sp. nov., an actinobacterium isolated from the gut of marine sandworm (). Kim, Bora Jun, Min Ok Yang, Ah-In Joe, Sung-Hong Joe, Hae-In Sung, Hojun Kim, Ki Hyun Kim, Hyun Jung Shin, Na-Ri RNA, Ribosomal, 16S Phylogeny Base Composition Fatty Acids Animals DNA, Bacterial Bacterial Typing Techniques Sequence Analysis, DNA Vitamin K 2 Nucleic Acid Hybridization Phospholipids Peptidoglycan Polychaeta Genome, Bacterial A novel Gram-stain-positive, facultatively anaerobic, non-motile, catalase-positive, oxidase-negative and ovoid cocci, designated as A1S7, was isolated from the gut of a marine sandworm (). Strain A1S7 exhibited optimal growth at temperatures of 20-30 ℃, pH 6-8 and in the presence of 2-4% (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain A1S7 belonged to the genus , exhibiting a similarity of 99.0% to KCTC 49873, followed by KCTC 19282 (98.8%), KCTC 49872 (98.4%), KACC 20518 (98.2%) and KACC 21120 (97.2%). The complete genome sequence of strain A1S7 revealed a genome size of 3360920 bp with a genomic G+C content of 70.1 mol%. The orthologous average nucleotide identity and the digital DNA-DNA hybridization values between strain A1S7 and KCTC 49873 were determined to be 89.5 and 37.2%, respectively. The major respiratory quinone was MK-8(H). The predominant fatty acids (>10%) included -C, C 8, C 9 and C. Polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phosphoglycolipid and three unknown polar lipids. The cell-wall peptidoglycan type was A1. The major whole-cell sugars were ribose, mannose and glucose. Based on phenotypic, phylogenetic, genotypic and chemotaxonomic properties, strain A1S7 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is A1S7 (=KCTC 49714 = JCM 36706). |
| format | Artículo científico |
| id | pubmed_39453699 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | International journal of systematic and evolutionary microbiology |
| record_format | pubmed |
| spellingShingle | sp. nov., an actinobacterium isolated from the gut of marine sandworm (). Kim, Bora Jun, Min Ok Yang, Ah-In Joe, Sung-Hong Joe, Hae-In Sung, Hojun Kim, Ki Hyun Kim, Hyun Jung Shin, Na-Ri RNA, Ribosomal, 16S Phylogeny Base Composition Fatty Acids Animals DNA, Bacterial Bacterial Typing Techniques Sequence Analysis, DNA Vitamin K 2 Nucleic Acid Hybridization Phospholipids Peptidoglycan Polychaeta Genome, Bacterial sp. nov., an actinobacterium isolated from the gut of marine sandworm (). Kim, Bora Jun, Min Ok Yang, Ah-In Joe, Sung-Hong Joe, Hae-In Sung, Hojun Kim, Ki Hyun Kim, Hyun Jung Shin, Na-Ri RNA, Ribosomal, 16S Phylogeny Base Composition Fatty Acids Animals DNA, Bacterial Bacterial Typing Techniques Sequence Analysis, DNA Vitamin K 2 Nucleic Acid Hybridization Phospholipids Peptidoglycan Polychaeta Genome, Bacterial A novel Gram-stain-positive, facultatively anaerobic, non-motile, catalase-positive, oxidase-negative and ovoid cocci, designated as A1S7, was isolated from the gut of a marine sandworm (). Strain A1S7 exhibited optimal growth at temperatures of 20-30 ℃, pH 6-8 and in the presence of 2-4% (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain A1S7 belonged to the genus , exhibiting a similarity of 99.0% to KCTC 49873, followed by KCTC 19282 (98.8%), KCTC 49872 (98.4%), KACC 20518 (98.2%) and KACC 21120 (97.2%). The complete genome sequence of strain A1S7 revealed a genome size of 3360920 bp with a genomic G+C content of 70.1 mol%. The orthologous average nucleotide identity and the digital DNA-DNA hybridization values between strain A1S7 and KCTC 49873 were determined to be 89.5 and 37.2%, respectively. The major respiratory quinone was MK-8(H). The predominant fatty acids (>10%) included -C, C 8, C 9 and C. Polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phosphoglycolipid and three unknown polar lipids. The cell-wall peptidoglycan type was A1. The major whole-cell sugars were ribose, mannose and glucose. Based on phenotypic, phylogenetic, genotypic and chemotaxonomic properties, strain A1S7 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is A1S7 (=KCTC 49714 = JCM 36706). |
| title | sp. nov., an actinobacterium isolated from the gut of marine sandworm (). |
| topic | RNA, Ribosomal, 16S Phylogeny Base Composition Fatty Acids Animals DNA, Bacterial Bacterial Typing Techniques Sequence Analysis, DNA Vitamin K 2 Nucleic Acid Hybridization Phospholipids Peptidoglycan Polychaeta Genome, Bacterial |
| url | https://pubmed.ncbi.nlm.nih.gov/39453699/ |