Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
The Journal of steroid biochemistry and molecular biology
2025
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39454895/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266287739699201 |
|---|---|
| author | Sebihi, Stellia Monperrus, Mathilde Coste, Pascale Huchet, Emmanuel Lingrand, Matthieu Glise, Stéphane Bouchard, Colin Ortiz-Zarragoitia, Maren Bolliet, Valérie |
| author_facet | Sebihi, Stellia Monperrus, Mathilde Coste, Pascale Huchet, Emmanuel Lingrand, Matthieu Glise, Stéphane Bouchard, Colin Ortiz-Zarragoitia, Maren Bolliet, Valérie Sebihi, Stellia Monperrus, Mathilde Coste, Pascale Huchet, Emmanuel Lingrand, Matthieu Glise, Stéphane Bouchard, Colin Ortiz-Zarragoitia, Maren Bolliet, Valérie |
| collection | PubMed - marine biology |
| contents | An optimized whole-body corticosteroid hormones quantification method by LC-MS/MS for assessing stress levels in European glass eels (Anguilla anguilla). Sebihi, Stellia Monperrus, Mathilde Coste, Pascale Huchet, Emmanuel Lingrand, Matthieu Glise, Stéphane Bouchard, Colin Ortiz-Zarragoitia, Maren Bolliet, Valérie Animals Tandem Mass Spectrometry Chromatography, Liquid Stress, Physiological Hydrocortisone Anguilla Diazepam Anti-Anxiety Agents Adrenal Cortex Hormones Corticosterone Liquid Chromatography-Mass Spectrometry The European eel (Anguilla anguilla) juvenile stage exhibits facultative estuarine migration. The causes of this behavior are yet unknown but it may have an impact on the population's fate by altering the sex ratio of the population. Recent studies have highlighted potential stress-related issues in glass eels settling in estuaries but studying stress response in small organisms requires sensitive, accurate and precise analytical methods. The aims of the present study are (i) to develop a whole-body Liquid Chromatography-Mass Spectrometry (LC-MS/MS) method for the simultaneous determination of several stress hormones in low-body mass fish; (ii) to apply this method to glass eels to study their responses to acute stress (iii) to test the effect of anxiolytics (diazepam) on these responses. Our results showed that enhanced LC-MS/MS analysis reduced detection limits and improved accuracy and precision for the quantification at the individual level. Following an acute stress, cortisol concentration significantly increased in glass eels and a 15 h diazepam exposure significantly reduced cortisol levels highlighting a marked anxiolytic effect on this species. |
| format | Artículo científico |
| id | pubmed_39454895 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | The Journal of steroid biochemistry and molecular biology |
| record_format | pubmed |
| spellingShingle | An optimized whole-body corticosteroid hormones quantification method by LC-MS/MS for assessing stress levels in European glass eels (Anguilla anguilla). Sebihi, Stellia Monperrus, Mathilde Coste, Pascale Huchet, Emmanuel Lingrand, Matthieu Glise, Stéphane Bouchard, Colin Ortiz-Zarragoitia, Maren Bolliet, Valérie Animals Tandem Mass Spectrometry Chromatography, Liquid Stress, Physiological Hydrocortisone Anguilla Diazepam Anti-Anxiety Agents Adrenal Cortex Hormones Corticosterone Liquid Chromatography-Mass Spectrometry An optimized whole-body corticosteroid hormones quantification method by LC-MS/MS for assessing stress levels in European glass eels (Anguilla anguilla). Sebihi, Stellia Monperrus, Mathilde Coste, Pascale Huchet, Emmanuel Lingrand, Matthieu Glise, Stéphane Bouchard, Colin Ortiz-Zarragoitia, Maren Bolliet, Valérie Animals Tandem Mass Spectrometry Chromatography, Liquid Stress, Physiological Hydrocortisone Anguilla Diazepam Anti-Anxiety Agents Adrenal Cortex Hormones Corticosterone Liquid Chromatography-Mass Spectrometry The European eel (Anguilla anguilla) juvenile stage exhibits facultative estuarine migration. The causes of this behavior are yet unknown but it may have an impact on the population's fate by altering the sex ratio of the population. Recent studies have highlighted potential stress-related issues in glass eels settling in estuaries but studying stress response in small organisms requires sensitive, accurate and precise analytical methods. The aims of the present study are (i) to develop a whole-body Liquid Chromatography-Mass Spectrometry (LC-MS/MS) method for the simultaneous determination of several stress hormones in low-body mass fish; (ii) to apply this method to glass eels to study their responses to acute stress (iii) to test the effect of anxiolytics (diazepam) on these responses. Our results showed that enhanced LC-MS/MS analysis reduced detection limits and improved accuracy and precision for the quantification at the individual level. Following an acute stress, cortisol concentration significantly increased in glass eels and a 15 h diazepam exposure significantly reduced cortisol levels highlighting a marked anxiolytic effect on this species. |
| title | An optimized whole-body corticosteroid hormones quantification method by LC-MS/MS for assessing stress levels in European glass eels (Anguilla anguilla). |
| topic | Animals Tandem Mass Spectrometry Chromatography, Liquid Stress, Physiological Hydrocortisone Anguilla Diazepam Anti-Anxiety Agents Adrenal Cortex Hormones Corticosterone Liquid Chromatography-Mass Spectrometry |
| url | https://pubmed.ncbi.nlm.nih.gov/39454895/ |