Salvato in:
| Autori principali: | , , , , , , |
|---|---|
| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
International journal of biological macromolecules
2024
|
| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/39454921/ |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1868266287739699200 |
|---|---|
| author | Espinoza, Daniela Laporte, Daniel Martínez, Fabián Sandino, Ana María Valdés, Natalia Moenne, Alejandra Imarai, Mónica |
| author_facet | Espinoza, Daniela Laporte, Daniel Martínez, Fabián Sandino, Ana María Valdés, Natalia Moenne, Alejandra Imarai, Mónica Espinoza, Daniela Laporte, Daniel Martínez, Fabián Sandino, Ana María Valdés, Natalia Moenne, Alejandra Imarai, Mónica |
| collection | PubMed - marine biology |
| contents | Lambda carrageenan displays antiviral activity against the infectious pancreatic necrosis virus (IPNV) by inhibiting viral replication and enhancing innate immunity in salmonid cells. Espinoza, Daniela Laporte, Daniel Martínez, Fabián Sandino, Ana María Valdés, Natalia Moenne, Alejandra Imarai, Mónica Animals Infectious pancreatic necrosis virus Carrageenan Virus Replication Antiviral Agents Immunity, Innate Cell Line Salmonidae Birnaviridae Infections RNA, Viral Fish Diseases Sulfated polysaccharide lambda carrageenan (λ-CGN) was evaluated for its antiviral effect against IPNV using the in vitro infection model of CHSE-214 salmonid cells. A plaque reduction lysis assay revealed that λ-CGN has an IC of 0.9 μg⋅mL, CC > 128 μg⋅mL and a Selectivity Index (SI) > 142. In comparison, iota, kappa carrageenans and lambda oligo-carrageenan (λ-OC) were less effective than λ-CGN against IPNV. λ-CGN showed no virucidal activity when applied directly to viral particles. Time of addition experiments showed that pre-treatment, co-treatment, and post-treatment with λ-CGN significantly reduced viral RNA copies in the cell supernatant. Additionally, a decrease in intracellular viral RNA was observed with pre-treatment and post-treatment, indicating an impact on different stages of viral replication. Polyacrylamide gel electrophoresis of IPNV genomic RNA in presence of λ-CGN showed a reduction in the level of viral genomic RNA. Confocal microscopy confirmed the intracellular localization of λ-CGN, suggesting that λ-CGN may inhibit the synthesis of IPNV genomic RNA. Moreover, cells pre-treated with λ-CGN showed an increased expression of innate immunity genes CXCL11, IL1β, IFNa, and IRF3. These findings highlight the need for further research to confirm the in vivo pharmacological potential of λ-CGN as a new antiviral agent in salmonids. |
| format | Artículo científico |
| id | pubmed_39454921 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | International journal of biological macromolecules |
| record_format | pubmed |
| spellingShingle | Lambda carrageenan displays antiviral activity against the infectious pancreatic necrosis virus (IPNV) by inhibiting viral replication and enhancing innate immunity in salmonid cells. Espinoza, Daniela Laporte, Daniel Martínez, Fabián Sandino, Ana María Valdés, Natalia Moenne, Alejandra Imarai, Mónica Animals Infectious pancreatic necrosis virus Carrageenan Virus Replication Antiviral Agents Immunity, Innate Cell Line Salmonidae Birnaviridae Infections RNA, Viral Fish Diseases Lambda carrageenan displays antiviral activity against the infectious pancreatic necrosis virus (IPNV) by inhibiting viral replication and enhancing innate immunity in salmonid cells. Espinoza, Daniela Laporte, Daniel Martínez, Fabián Sandino, Ana María Valdés, Natalia Moenne, Alejandra Imarai, Mónica Animals Infectious pancreatic necrosis virus Carrageenan Virus Replication Antiviral Agents Immunity, Innate Cell Line Salmonidae Birnaviridae Infections RNA, Viral Fish Diseases Sulfated polysaccharide lambda carrageenan (λ-CGN) was evaluated for its antiviral effect against IPNV using the in vitro infection model of CHSE-214 salmonid cells. A plaque reduction lysis assay revealed that λ-CGN has an IC of 0.9 μg⋅mL, CC > 128 μg⋅mL and a Selectivity Index (SI) > 142. In comparison, iota, kappa carrageenans and lambda oligo-carrageenan (λ-OC) were less effective than λ-CGN against IPNV. λ-CGN showed no virucidal activity when applied directly to viral particles. Time of addition experiments showed that pre-treatment, co-treatment, and post-treatment with λ-CGN significantly reduced viral RNA copies in the cell supernatant. Additionally, a decrease in intracellular viral RNA was observed with pre-treatment and post-treatment, indicating an impact on different stages of viral replication. Polyacrylamide gel electrophoresis of IPNV genomic RNA in presence of λ-CGN showed a reduction in the level of viral genomic RNA. Confocal microscopy confirmed the intracellular localization of λ-CGN, suggesting that λ-CGN may inhibit the synthesis of IPNV genomic RNA. Moreover, cells pre-treated with λ-CGN showed an increased expression of innate immunity genes CXCL11, IL1β, IFNa, and IRF3. These findings highlight the need for further research to confirm the in vivo pharmacological potential of λ-CGN as a new antiviral agent in salmonids. |
| title | Lambda carrageenan displays antiviral activity against the infectious pancreatic necrosis virus (IPNV) by inhibiting viral replication and enhancing innate immunity in salmonid cells. |
| topic | Animals Infectious pancreatic necrosis virus Carrageenan Virus Replication Antiviral Agents Immunity, Innate Cell Line Salmonidae Birnaviridae Infections RNA, Viral Fish Diseases |
| url | https://pubmed.ncbi.nlm.nih.gov/39454921/ |