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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Viruses
2024
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| Materias: | |
| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/39459847/ |
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| _version_ | 1868266287735504897 |
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| author | Cao, Helong Zhang, Dongzhuo Mu, Guanghui Wu, Siting Tu, Yurong Qin, Qiwei Wei, Jingguang |
| author_facet | Cao, Helong Zhang, Dongzhuo Mu, Guanghui Wu, Siting Tu, Yurong Qin, Qiwei Wei, Jingguang Cao, Helong Zhang, Dongzhuo Mu, Guanghui Wu, Siting Tu, Yurong Qin, Qiwei Wei, Jingguang |
| collection | PubMed - marine biology |
| contents | Isolation, Identification and Genomic Analysis of Orange-Spotted Grouper Iridovirus Hainan Strain in China. Cao, Helong Zhang, Dongzhuo Mu, Guanghui Wu, Siting Tu, Yurong Qin, Qiwei Wei, Jingguang Animals Fish Diseases China Iridovirus Genome, Viral Phylogeny DNA Virus Infections Aquaculture Genomics Bass The orange-spotted grouper () is an important mariculture fish in China. However, in recent years, with the rapid development of aquaculture activities, outbreaks of viral diseases have affected the grouper aquaculture industry, causing severe economic losses. In the present study, we isolated and identified a virus from diseased, orange-spotted groupers from an aquaculture farm in Hainan Province, China. The isolated virus was identified as orange-spotted grouper iridovirus, hence named the orange-spotted grouper iridovirus Hainan strain (OSGIV-HN-2018-001). OSGIV-HN-2018-001 induces a cytopathic effect after the infection of mandarin fish () brain clonal passage (SBC) cells. In addition, the cytoplasm of the OSGIV-HN-2018-001-infected SBC cells was found to contain a large number of hexagonal virus particles with a diameter of approximately 134 nm. Using the Illumina NovaSeq system, we assembled the sequence data and annotated the complete genome of OSGIV-HN-2018-001 (GenBank accession number: PP974677), which consisted of 110,699 bp and contained 122 open reading frames (ORFs). Phylogenetic tree analysis showed that OSGIV-HN-2018-001 was most closely related to ISKNV-ASB-23. The cumulative mortality rate of groupers infected with OSGIV-HN-2018-001 reached 100% on day 8. The spleens were enlarged and blackened after the dissection of the dying groupers. These results contribute to the understanding of the molecular regulatory mechanism of the iridovirus infection and provide a basis for iridovirus prevention. |
| format | Artículo científico |
| id | pubmed_39459847 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Viruses |
| record_format | pubmed |
| spellingShingle | Isolation, Identification and Genomic Analysis of Orange-Spotted Grouper Iridovirus Hainan Strain in China. Cao, Helong Zhang, Dongzhuo Mu, Guanghui Wu, Siting Tu, Yurong Qin, Qiwei Wei, Jingguang Animals Fish Diseases China Iridovirus Genome, Viral Phylogeny DNA Virus Infections Aquaculture Genomics Bass Isolation, Identification and Genomic Analysis of Orange-Spotted Grouper Iridovirus Hainan Strain in China. Cao, Helong Zhang, Dongzhuo Mu, Guanghui Wu, Siting Tu, Yurong Qin, Qiwei Wei, Jingguang Animals Fish Diseases China Iridovirus Genome, Viral Phylogeny DNA Virus Infections Aquaculture Genomics Bass The orange-spotted grouper () is an important mariculture fish in China. However, in recent years, with the rapid development of aquaculture activities, outbreaks of viral diseases have affected the grouper aquaculture industry, causing severe economic losses. In the present study, we isolated and identified a virus from diseased, orange-spotted groupers from an aquaculture farm in Hainan Province, China. The isolated virus was identified as orange-spotted grouper iridovirus, hence named the orange-spotted grouper iridovirus Hainan strain (OSGIV-HN-2018-001). OSGIV-HN-2018-001 induces a cytopathic effect after the infection of mandarin fish () brain clonal passage (SBC) cells. In addition, the cytoplasm of the OSGIV-HN-2018-001-infected SBC cells was found to contain a large number of hexagonal virus particles with a diameter of approximately 134 nm. Using the Illumina NovaSeq system, we assembled the sequence data and annotated the complete genome of OSGIV-HN-2018-001 (GenBank accession number: PP974677), which consisted of 110,699 bp and contained 122 open reading frames (ORFs). Phylogenetic tree analysis showed that OSGIV-HN-2018-001 was most closely related to ISKNV-ASB-23. The cumulative mortality rate of groupers infected with OSGIV-HN-2018-001 reached 100% on day 8. The spleens were enlarged and blackened after the dissection of the dying groupers. These results contribute to the understanding of the molecular regulatory mechanism of the iridovirus infection and provide a basis for iridovirus prevention. |
| title | Isolation, Identification and Genomic Analysis of Orange-Spotted Grouper Iridovirus Hainan Strain in China. |
| topic | Animals Fish Diseases China Iridovirus Genome, Viral Phylogeny DNA Virus Infections Aquaculture Genomics Bass |
| url | https://pubmed.ncbi.nlm.nih.gov/39459847/ |