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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Antonie van Leeuwenhoek
2024
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39472387/ |
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| _version_ | 1868266287720824833 |
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| author | Wang, Hong-Chuan Huang, Meng-Han Guo, Dan-Yuan Liu, Wen-Li Yang, Zi-Wen Zhang, Dao-Feng Li, Wen-Jun |
| author_facet | Wang, Hong-Chuan Huang, Meng-Han Guo, Dan-Yuan Liu, Wen-Li Yang, Zi-Wen Zhang, Dao-Feng Li, Wen-Jun Wang, Hong-Chuan Huang, Meng-Han Guo, Dan-Yuan Liu, Wen-Li Yang, Zi-Wen Zhang, Dao-Feng Li, Wen-Jun |
| collection | PubMed - marine biology |
| contents | Roseobacter sinensis sp. nov., a marine bacterium capable to synthesize arachidonic acid. Wang, Hong-Chuan Huang, Meng-Han Guo, Dan-Yuan Liu, Wen-Li Yang, Zi-Wen Zhang, Dao-Feng Li, Wen-Jun Roseobacter Phylogeny RNA, Ribosomal, 16S Seawater Base Composition Fatty Acids China DNA, Bacterial Arachidonic Acid Bacterial Typing Techniques Phospholipids Sequence Analysis, DNA Ubiquinone Strain WL0113 was isolated from surface seawater of the coast of Lianyungang, Jiangsu province, PR China. Strain WL0113 shared highest 16S rRNA gene sequence similarity with Roseobacter insulae YSTF-M11 (98.8%), followed by R. cerasinus AI77 (98.8%), R. ponti MM-7 (98.0%). Strain WL0113 was Gram-stain-negative, cream, aerobic, non-motile and coccoid- to oval-shaped, and able to grow at pH 6.5-9.0 (optimum, pH 7.0-8.0), at 10-37 °C (optimum, 28 °C) and in the presence of 1-5% (w/v; optimum, 2.5%) NaCl. Ubiquinone-10 was detected as dominant. The main fatty acids (> 5%) of the strain WL0113 were C, iso-C 3OH, Cω6,9,12,15c (arachidonic acid), and summed feature 8 (Cω7c and/or Cω6c). The major polar lipids include phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, glycophospholipid, unknown aminolipid, unknown phospholipid, and two unknown polar lipids. The ANI and dDDH values between strain WL0113 and Roseobacter cerasinus were 80.4% and 23.0%, respectively. The genomic DNA G + C content of strain WL0113 was 63.1%. Based on these data, it is proposed that strain WL0113 represent novel species of the genus Roseobacter, for which the name Roseobacter sinensis sp. nov. is proposed. The type strain is WL0113 (= GDMCC 1.3082 = JCM 35567). |
| format | Artículo científico |
| id | pubmed_39472387 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Antonie van Leeuwenhoek |
| record_format | pubmed |
| spellingShingle | Roseobacter sinensis sp. nov., a marine bacterium capable to synthesize arachidonic acid. Wang, Hong-Chuan Huang, Meng-Han Guo, Dan-Yuan Liu, Wen-Li Yang, Zi-Wen Zhang, Dao-Feng Li, Wen-Jun Roseobacter Phylogeny RNA, Ribosomal, 16S Seawater Base Composition Fatty Acids China DNA, Bacterial Arachidonic Acid Bacterial Typing Techniques Phospholipids Sequence Analysis, DNA Ubiquinone Roseobacter sinensis sp. nov., a marine bacterium capable to synthesize arachidonic acid. Wang, Hong-Chuan Huang, Meng-Han Guo, Dan-Yuan Liu, Wen-Li Yang, Zi-Wen Zhang, Dao-Feng Li, Wen-Jun Roseobacter Phylogeny RNA, Ribosomal, 16S Seawater Base Composition Fatty Acids China DNA, Bacterial Arachidonic Acid Bacterial Typing Techniques Phospholipids Sequence Analysis, DNA Ubiquinone Strain WL0113 was isolated from surface seawater of the coast of Lianyungang, Jiangsu province, PR China. Strain WL0113 shared highest 16S rRNA gene sequence similarity with Roseobacter insulae YSTF-M11 (98.8%), followed by R. cerasinus AI77 (98.8%), R. ponti MM-7 (98.0%). Strain WL0113 was Gram-stain-negative, cream, aerobic, non-motile and coccoid- to oval-shaped, and able to grow at pH 6.5-9.0 (optimum, pH 7.0-8.0), at 10-37 °C (optimum, 28 °C) and in the presence of 1-5% (w/v; optimum, 2.5%) NaCl. Ubiquinone-10 was detected as dominant. The main fatty acids (> 5%) of the strain WL0113 were C, iso-C 3OH, Cω6,9,12,15c (arachidonic acid), and summed feature 8 (Cω7c and/or Cω6c). The major polar lipids include phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, glycophospholipid, unknown aminolipid, unknown phospholipid, and two unknown polar lipids. The ANI and dDDH values between strain WL0113 and Roseobacter cerasinus were 80.4% and 23.0%, respectively. The genomic DNA G + C content of strain WL0113 was 63.1%. Based on these data, it is proposed that strain WL0113 represent novel species of the genus Roseobacter, for which the name Roseobacter sinensis sp. nov. is proposed. The type strain is WL0113 (= GDMCC 1.3082 = JCM 35567). |
| title | Roseobacter sinensis sp. nov., a marine bacterium capable to synthesize arachidonic acid. |
| topic | Roseobacter Phylogeny RNA, Ribosomal, 16S Seawater Base Composition Fatty Acids China DNA, Bacterial Arachidonic Acid Bacterial Typing Techniques Phospholipids Sequence Analysis, DNA Ubiquinone |
| url | https://pubmed.ncbi.nlm.nih.gov/39472387/ |