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| Sprache: | en |
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Carbohydrate polymers
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/39487004/ |
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| author | Kokoulin, Maxim S Kuzmich, Alexandra S Filshtein, Alina P Prassolov, Vladimir S Romanenko, Lyudmila A |
| author_facet | Kokoulin, Maxim S Kuzmich, Alexandra S Filshtein, Alina P Prassolov, Vladimir S Romanenko, Lyudmila A Kokoulin, Maxim S Kuzmich, Alexandra S Filshtein, Alina P Prassolov, Vladimir S Romanenko, Lyudmila A |
| collection | PubMed - marine biology |
| contents | Capsular polysaccharide from the marine bacterium Cobetia marina induces apoptosis via both caspase-dependent and mitochondria-mediated pathways in HL-60 cells. Kokoulin, Maxim S Kuzmich, Alexandra S Filshtein, Alina P Prassolov, Vladimir S Romanenko, Lyudmila A Humans HL-60 Cells Apoptosis Mitochondria Polysaccharides, Bacterial Caspases Membrane Potential, Mitochondrial Cell Proliferation Reactive Oxygen Species Signal Transduction Antineoplastic Agents Proto-Oncogene Proteins c-bcl-2 p38 Mitogen-Activated Protein Kinases Tumor Necrosis Factor-alpha Bacterial Capsules In the present study, we investigated the antiproliferative effect of the capsular polysaccharide (CPS) from marine Gram-negative bacterium Cobetia marina (formerly C. pacifica) KMM 3878 against human leukemia cells in vitro and the potential molecular mechanism underlying this activity. Our results showed that the CPS could inhibit the proliferation of HL-60 cells in a dose-dependent manner with no effect on normal PBMC cells. HL-60 cells treated with the CPS exhibited typical morphologic and biochemical signs of apoptosis. We found that the CPS caused the collapse of mitochondrial transmembrane potential (ΔΨm), activated caspases-8,-9, and - 3, decreased the ratio of anti-apoptotic Bcl-2 and pro-apoptotic Bax proteins, increased ROS production and TNF-α secretion, and stimulated phosphorylation of p38 MAPK and p53 in HL-60 cells. Taken together, these data suggest that both extracellular and intracellular signaling pathways contribute to the CPS-induced apoptosis in HL-60 cells. |
| format | Artículo científico |
| id | pubmed_39487004 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Carbohydrate polymers |
| record_format | pubmed |
| spellingShingle | Capsular polysaccharide from the marine bacterium Cobetia marina induces apoptosis via both caspase-dependent and mitochondria-mediated pathways in HL-60 cells. Kokoulin, Maxim S Kuzmich, Alexandra S Filshtein, Alina P Prassolov, Vladimir S Romanenko, Lyudmila A Humans HL-60 Cells Apoptosis Mitochondria Polysaccharides, Bacterial Caspases Membrane Potential, Mitochondrial Cell Proliferation Reactive Oxygen Species Signal Transduction Antineoplastic Agents Proto-Oncogene Proteins c-bcl-2 p38 Mitogen-Activated Protein Kinases Tumor Necrosis Factor-alpha Bacterial Capsules Capsular polysaccharide from the marine bacterium Cobetia marina induces apoptosis via both caspase-dependent and mitochondria-mediated pathways in HL-60 cells. Kokoulin, Maxim S Kuzmich, Alexandra S Filshtein, Alina P Prassolov, Vladimir S Romanenko, Lyudmila A Humans HL-60 Cells Apoptosis Mitochondria Polysaccharides, Bacterial Caspases Membrane Potential, Mitochondrial Cell Proliferation Reactive Oxygen Species Signal Transduction Antineoplastic Agents Proto-Oncogene Proteins c-bcl-2 p38 Mitogen-Activated Protein Kinases Tumor Necrosis Factor-alpha Bacterial Capsules In the present study, we investigated the antiproliferative effect of the capsular polysaccharide (CPS) from marine Gram-negative bacterium Cobetia marina (formerly C. pacifica) KMM 3878 against human leukemia cells in vitro and the potential molecular mechanism underlying this activity. Our results showed that the CPS could inhibit the proliferation of HL-60 cells in a dose-dependent manner with no effect on normal PBMC cells. HL-60 cells treated with the CPS exhibited typical morphologic and biochemical signs of apoptosis. We found that the CPS caused the collapse of mitochondrial transmembrane potential (ΔΨm), activated caspases-8,-9, and - 3, decreased the ratio of anti-apoptotic Bcl-2 and pro-apoptotic Bax proteins, increased ROS production and TNF-α secretion, and stimulated phosphorylation of p38 MAPK and p53 in HL-60 cells. Taken together, these data suggest that both extracellular and intracellular signaling pathways contribute to the CPS-induced apoptosis in HL-60 cells. |
| title | Capsular polysaccharide from the marine bacterium Cobetia marina induces apoptosis via both caspase-dependent and mitochondria-mediated pathways in HL-60 cells. |
| topic | Humans HL-60 Cells Apoptosis Mitochondria Polysaccharides, Bacterial Caspases Membrane Potential, Mitochondrial Cell Proliferation Reactive Oxygen Species Signal Transduction Antineoplastic Agents Proto-Oncogene Proteins c-bcl-2 p38 Mitogen-Activated Protein Kinases Tumor Necrosis Factor-alpha Bacterial Capsules |
| url | https://pubmed.ncbi.nlm.nih.gov/39487004/ |